id: Q7SCN3
gene_symbol: NCU08595
locus_tag: NCU08595
taxon:
  id: NCBITaxon:367110
  label: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
status: COMPLETE
description: NCU08595 is a Rpf2-family ribosome assembly factor with a Brix RNA-binding domain. By conservation
  with characterized fungal Rpf2, it associates with Rrs1, binds 5S rRNA and promotes incorporation of
  the 5S ribonucleoprotein particle into nucleolar large-subunit precursors. This assembly step supports
  large-subunit rRNA maturation.
source_documents:
- genes/NEUCR/NCU08595/NCU08595-protnlm-source.json
- genes/NEUCR/NCU08595/NCU08595-uniprot.txt
- genes/NEUCR/NCU08595/NCU08595-ai-review.yaml
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-09
  source_reference_id: file:NEUCR/NCU08595/NCU08595-protnlm-source.json
  predicted_term:
    id: GO:0000470
    label: maturation of LSU-rRNA
  predicted_term_type: GO_BP
  review:
    assessment: LSP
    confidence_score: 2
    summary: The Rpf2-specific family assignment, beyond the generic Brix domain, supports transfer from
      characterized fungal Rpf2-Rrs1 complexes. Structural and biochemical studies establish 5S rRNA recruitment
      and downstream large-subunit maturation. GOA already includes maturation of LSU-rRNA from the tricistronic
      precursor (GO:0000463), making this general LSU-rRNA maturation prediction less precise. Participation
      is through ribosome assembly and does not imply intrinsic nuclease chemistry.
    supported_by:
    - reference_id: PMID:25855814
      supporting_text: Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation.
    - reference_id: PMID:25855814
      supporting_text: 'gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly

        to 5S rRNA.'
    - reference_id: file:NEUCR/NCU08595/NCU08595-uniprot.txt
      supporting_text: DR   InterPro; IPR039770; Rpf2.
references:
- &id001
  id: PMID:25855814
  title: Structural and functional analysis of the Rpf2-Rrs1 complex in ribosome biogenesis.
  full_text_unavailable: false
- *id001
- id: file:NEUCR/NCU08595/NCU08595-uniprot.txt
  title: 'UniProt record for NCU08595: domain and family evidence'
- id: file:NEUCR/NCU08595/NCU08595-protnlm-source.json
  title: Frozen live ProtNLM2 API response for NCU08595