NCU08990

UniProt ID: Q7S2X9
Organism: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Review Status: COMPLETE
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Gene Description

NCU08990 encodes eL39, a small basic structural protein of the cytosolic large ribosomal subunit. It is present in the experimentally determined Neurospora 80S ribosome and participates in cytoplasmic protein synthesis. Characterized fungal eL39 contributes to ribosome integrity and translational accuracy.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0002181 cytoplasmic translation
IBA
GO_REF:0000033 		ACCEPT
Summary: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9.
Reason: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9. Its eL39 domain and placement in the translating large subunit directly support structural ribosomal function and cytoplasmic translation; yeast L39 perturbations also impair translational accuracy.
Supporting Evidence:
PMID:34815343
We elucidated CHX's mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.
PMID:10852723
Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type.
GO:0003735 structural constituent of ribosome
IBA
GO_REF:0000033 		ACCEPT
Summary: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9.
Reason: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9. Its eL39 domain and placement in the translating large subunit directly support structural ribosomal function and cytoplasmic translation; yeast L39 perturbations also impair translational accuracy.
Supporting Evidence:
PMID:34815343
We elucidated CHX's mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.
PMID:10852723
Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type.
GO:0003735 structural constituent of ribosome
IEA
GO_REF:0000002 		ACCEPT
Summary: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9.
Reason: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9. Its eL39 domain and placement in the translating large subunit directly support structural ribosomal function and cytoplasmic translation; yeast L39 perturbations also impair translational accuracy.
Supporting Evidence:
PMID:34815343
We elucidated CHX's mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.
PMID:10852723
Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type.
GO:0005840 ribosome
IEA
GO_REF:0000002 		MODIFY
Summary: The structural assignment is specifically to the cytosolic large ribosomal subunit.
Reason: The structural assignment is specifically to the cytosolic large ribosomal subunit. The generic ribosome term omits this experimentally resolved compartment and subunit.
Supporting Evidence:
PMID:34815343
We elucidated CHX's mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.
GO:0006412 translation
IEA
GO_REF:0000002 		MODIFY
Summary: The Neurospora 80S ribosome structure and eL39 family establish cytoplasmic translation specifically.
Reason: The Neurospora 80S ribosome structure and eL39 family establish cytoplasmic translation specifically. The generic translation term can be replaced by its supported compartment-specific form.
Proposed replacements: cytoplasmic translation
Supporting Evidence:
PMID:34815343
We elucidated CHX's mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.
GO:0022625 cytosolic large ribosomal subunit
IBA
GO_REF:0000033 		ACCEPT
Summary: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9.
Reason: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9. Its eL39 domain and placement in the translating large subunit directly support structural ribosomal function and cytoplasmic translation; yeast L39 perturbations also impair translational accuracy.
Supporting Evidence:
PMID:34815343
We elucidated CHX's mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.
PMID:10852723
Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type.
GO:0022625 cytosolic large ribosomal subunit
IEA
GO_REF:0000107 		ACCEPT
Summary: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9.
Reason: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9. Its eL39 domain and placement in the translating large subunit directly support structural ribosomal function and cytoplasmic translation; yeast L39 perturbations also impair translational accuracy.
Supporting Evidence:
PMID:34815343
We elucidated CHX's mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.
PMID:10852723
Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type.
GO:0030684 preribosome
IEA
GO_REF:0000107 		ACCEPT
Summary: eL39 is assembled into the large ribosomal subunit before mature cytoplasmic translation.
Reason: eL39 is assembled into the large ribosomal subunit before mature cytoplasmic translation. The curated fungal orthology transfer to a preribosome is consistent with this conserved assembly route; the target mature structure corroborates ribosomal identity but is not itself a direct preribosome assay.
Supporting Evidence:
PMID:34815343
We elucidated CHX's mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.
PMID:10852723
Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type.

Core Functions

NCU08990 encodes eL39, a small basic structural protein of the cytosolic large ribosomal subunit. It is present in the experimentally determined Neurospora 80S ribosome and participates in cytoplasmic protein synthesis. Characterized fungal eL39 contributes to ribosome integrity and translational accuracy.

Molecular Function:
structural constituent of ribosome
Directly Involved In:
cytoplasmic translation
In Complex:
cytosolic large ribosomal subunit
Supporting Evidence:
  • PMID:34815343
    We elucidated CHX's mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.
  • PMID:10852723
    Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type.
  • file:NEUCR/NCU08990/NCU08990-uniprot.txt
    DR InterPro; IPR000077; Ribosomal_eL39.

References

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External Prediction Reviews

These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.

ProtNLM2 External predictions

View prediction review YAML · NCU08990-protnlm-predictions-review.yaml · Review status: COMPLETE

NCU08990 encodes eL39, a small basic structural protein of the cytosolic large ribosomal subunit. It is present in the experimentally determined Neurospora 80S ribosome and participates in cytoplasmic protein synthesis. Characterized fungal eL39 contributes to ribosome integrity and translational accuracy.

Source documents: genes/NEUCR/NCU08990/NCU08990-protnlm-source.json · genes/NEUCR/NCU08990/NCU08990-uniprot.txt · genes/NEUCR/NCU08990/NCU08990-ai-review.yaml

Review score: 2 = concordant with evidence; 1 = uncertain; 0 = discordant with evidence. This is an assessment score, not a model probability.

GO:0005840 ribosome GO_CC
LSP — Less precise than existing annotation Review score: 2/2
Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-09 · file:NEUCR/NCU08990/NCU08990-protnlm-source.json
Review rationale: Neurospora cryo-EM structure 7R81 identifies this exact protein as eL39, entity 74/chain VB (author n1), in the translating large ribosomal subunit. The eL39-specific domain agrees with the direct structural assignment. GOA already provides cytosolic large ribosomal subunit (GO:0022625), a cellular-component descendant of ribosome. The emitted ribosome prediction is therefore correct but less precise than the established compartment and subunit assignment.
Supporting Evidence:
  • PMID:34815343: "We elucidated CHX's mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution."
  • PMID:10852723: "Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type."
  • file:NEUCR/NCU08990/NCU08990-uniprot.txt: "DR InterPro; IPR000077; Ribosomal_eL39."
  • file:NEUCR/NCU08990/NCU08990-7R81-entity74.json: ""pdbx_strand_id": "n1" ... "pdbx_description": "60S ribosomal protein L39" ... "reference_database_accession": "Q7S2X9""
GO:1990904 ribonucleoprotein complex GO_CC
LSP — Less precise than existing annotation Review score: 2/2
Prediction method: ProtNLM2 · Version: UniProt API snapshot 2026-09-09 · file:NEUCR/NCU08990/NCU08990-protnlm-source.json
Review rationale: Neurospora cryo-EM structure 7R81 identifies this exact protein as eL39, entity 74/chain VB (author n1), in the translating large ribosomal subunit. The eL39-specific domain agrees with the direct structural assignment. GOA already provides cytosolic large ribosomal subunit (GO:0022625), a cellular-component descendant of ribonucleoprotein complex. The emitted ribonucleoprotein complex prediction is therefore correct but less precise than the established compartment and subunit assignment.
Supporting Evidence:
  • PMID:34815343: "We elucidated CHX's mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution."
  • PMID:10852723: "Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type."
  • file:NEUCR/NCU08990/NCU08990-uniprot.txt: "DR InterPro; IPR000077; Ribosomal_eL39."
  • file:NEUCR/NCU08990/NCU08990-7R81-entity74.json: ""pdbx_strand_id": "n1" ... "pdbx_description": "60S ribosomal protein L39" ... "reference_database_accession": "Q7S2X9""

Deep Research

Falcon

(NCU08990-deep-research-falcon.md)

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📚 Additional Documentation

Notes

(NCU08990-notes.md)

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Protnlm Location Review

(NCU08990-protnlm-location-review.md)

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📄 View Raw YAML

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