id: Q7S2X9 gene_symbol: NCU08990 product_type: PROTEIN status: COMPLETE taxon: id: NCBITaxon:367110 label: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) description: NCU08990 encodes eL39, a small basic structural protein of the cytosolic large ribosomal subunit. It is present in the experimentally determined Neurospora 80S ribosome and participates in cytoplasmic protein synthesis. Characterized fungal eL39 contributes to ribosome integrity and translational accuracy. existing_annotations: - term: &id001 id: GO:0002181 label: cytoplasmic translation evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: involved_in supporting_entities: - MGI:MGI:1914498 - MGI:MGI:1915422 - PANTHER:PTN000463054 - UniProtKB:P62891 review: summary: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9. action: ACCEPT reason: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9. Its eL39 domain and placement in the translating large subunit directly support structural ribosomal function and cytoplasmic translation; yeast L39 perturbations also impair translational accuracy. supported_by: - reference_id: PMID:34815343 supporting_text: 'We elucidated CHX''s mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.' - reference_id: PMID:10852723 supporting_text: 'Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type.' - term: id: GO:0003735 label: structural constituent of ribosome evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: enables supporting_entities: - AGI_LocusCode:AT2G25210 - AGI_LocusCode:AT3G02190 - MGI:MGI:1914498 - MGI:MGI:1915422 - PANTHER:PTN000463054 - UniProtKB:P62891 review: summary: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9. action: ACCEPT reason: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9. Its eL39 domain and placement in the translating large subunit directly support structural ribosomal function and cytoplasmic translation; yeast L39 perturbations also impair translational accuracy. supported_by: - reference_id: PMID:34815343 supporting_text: 'We elucidated CHX''s mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.' - reference_id: PMID:10852723 supporting_text: 'Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type.' - term: &id002 id: GO:0003735 label: structural constituent of ribosome evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: enables supporting_entities: - InterPro:IPR000077 review: summary: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9. action: ACCEPT reason: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9. Its eL39 domain and placement in the translating large subunit directly support structural ribosomal function and cytoplasmic translation; yeast L39 perturbations also impair translational accuracy. supported_by: - reference_id: PMID:34815343 supporting_text: 'We elucidated CHX''s mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.' - reference_id: PMID:10852723 supporting_text: 'Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type.' - term: id: GO:0005840 label: ribosome evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: located_in supporting_entities: - InterPro:IPR000077 review: summary: The structural assignment is specifically to the cytosolic large ribosomal subunit. action: MODIFY reason: The structural assignment is specifically to the cytosolic large ribosomal subunit. The generic ribosome term omits this experimentally resolved compartment and subunit. supported_by: - reference_id: PMID:34815343 supporting_text: 'We elucidated CHX''s mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.' proposed_replacement_terms: - id: GO:0022625 label: cytosolic large ribosomal subunit - term: id: GO:0006412 label: translation evidence_type: IEA original_reference_id: GO_REF:0000002 qualifier: involved_in supporting_entities: - InterPro:IPR000077 review: summary: The Neurospora 80S ribosome structure and eL39 family establish cytoplasmic translation specifically. action: MODIFY reason: The Neurospora 80S ribosome structure and eL39 family establish cytoplasmic translation specifically. The generic translation term can be replaced by its supported compartment-specific form. supported_by: - reference_id: PMID:34815343 supporting_text: 'We elucidated CHX''s mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.' proposed_replacement_terms: - id: GO:0002181 label: cytoplasmic translation - term: id: GO:0022625 label: cytosolic large ribosomal subunit evidence_type: IBA original_reference_id: GO_REF:0000033 qualifier: part_of supporting_entities: - MGI:MGI:1914498 - MGI:MGI:1915422 - PANTHER:PTN000463054 - PomBase:SPCC663.04 - RGD:3593 - SGD:S000003725 - UniProtKB:C0H4H3 - UniProtKB:P62891 - UniProtKB:Q96EH5 review: summary: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9. action: ACCEPT reason: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9. Its eL39 domain and placement in the translating large subunit directly support structural ribosomal function and cytoplasmic translation; yeast L39 perturbations also impair translational accuracy. supported_by: - reference_id: PMID:34815343 supporting_text: 'We elucidated CHX''s mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.' - reference_id: PMID:10852723 supporting_text: 'Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type.' - term: &id003 id: GO:0022625 label: cytosolic large ribosomal subunit evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: part_of supporting_entities: - UniProtKB:P04650 - sgd:YJL189W review: summary: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9. action: ACCEPT reason: The Neurospora 80S structure 7R81 contains eL39 as entity 74, chain VB (author n1), explicitly mapped to Q7S2X9. Its eL39 domain and placement in the translating large subunit directly support structural ribosomal function and cytoplasmic translation; yeast L39 perturbations also impair translational accuracy. supported_by: - reference_id: PMID:34815343 supporting_text: 'We elucidated CHX''s mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.' - reference_id: PMID:10852723 supporting_text: 'Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type.' - term: id: GO:0030684 label: preribosome evidence_type: IEA original_reference_id: GO_REF:0000107 qualifier: part_of supporting_entities: - UniProtKB:P05767 - PomBase:SPCC663.04.1:pep review: summary: eL39 is assembled into the large ribosomal subunit before mature cytoplasmic translation. action: ACCEPT reason: eL39 is assembled into the large ribosomal subunit before mature cytoplasmic translation. The curated fungal orthology transfer to a preribosome is consistent with this conserved assembly route; the target mature structure corroborates ribosomal identity but is not itself a direct preribosome assay. supported_by: - reference_id: PMID:34815343 supporting_text: 'We elucidated CHX''s mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.' - reference_id: PMID:10852723 supporting_text: 'Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type.' references: - id: GO_REF:0000002 title: Gene Ontology annotation through association of InterPro records with GO terms findings: [] - id: GO_REF:0000033 title: Annotation inferences using phylogenetic trees findings: [] - id: GO_REF:0000107 title: Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara findings: [] - id: PMID:34815343 title: Structure of the translating Neurospora ribosome arrested by cycloheximide. full_text_unavailable: true - id: PMID:10852723 title: Yeast ribosomal protein L24 affects the kinetics of protein synthesis and ribosomal protein L39 improves translational accuracy, while mutants lacking both remain viable. full_text_unavailable: true - id: file:NEUCR/NCU08990/NCU08990-uniprot.txt title: 'UniProt record for NCU08990: domain and family evidence' core_functions: - description: NCU08990 encodes eL39, a small basic structural protein of the cytosolic large ribosomal subunit. It is present in the experimentally determined Neurospora 80S ribosome and participates in cytoplasmic protein synthesis. Characterized fungal eL39 contributes to ribosome integrity and translational accuracy. supported_by: - reference_id: PMID:34815343 supporting_text: 'We elucidated CHX''s mechanism of action based on the cryo-electron microscopy structure of actively translating Neurospora crassa ribosomes bound with CHX at 2.7-Å resolution.' - reference_id: PMID:10852723 supporting_text: 'Each of the two L39 mutants displayed a 4-fold increase of their error frequencies over the wild type.' - reference_id: file:NEUCR/NCU08990/NCU08990-uniprot.txt supporting_text: DR InterPro; IPR000077; Ribosomal_eL39. directly_involved_in: - *id001 molecular_function: *id002 in_complex: *id003