id: Q7S2X9
gene_symbol: NCU08990
locus_tag: NCU08990
taxon:
  id: NCBITaxon:367110
  label: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
status: COMPLETE
description: NCU08990 encodes eL39, a small basic structural protein of the cytosolic large ribosomal
  subunit. It is present in the experimentally determined Neurospora 80S ribosome and participates in
  cytoplasmic protein synthesis. Characterized fungal eL39 contributes to ribosome integrity and translational
  accuracy.
source_documents:
- genes/NEUCR/NCU08990/NCU08990-protnlm-source.json
- genes/NEUCR/NCU08990/NCU08990-uniprot.txt
- genes/NEUCR/NCU08990/NCU08990-ai-review.yaml
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-09
  source_reference_id: file:NEUCR/NCU08990/NCU08990-protnlm-source.json
  predicted_term:
    id: GO:0005840
    label: ribosome
  predicted_term_type: GO_CC
  review:
    assessment: LSP
    confidence_score: 2
    summary: Neurospora cryo-EM structure 7R81 identifies this exact protein as eL39, entity 74/chain
      VB (author n1), in the translating large ribosomal subunit. The eL39-specific domain agrees with
      the direct structural assignment. GOA already provides cytosolic large ribosomal subunit (GO:0022625),
      a cellular-component descendant of ribosome. The emitted ribosome prediction is therefore correct
      but less precise than the established compartment and subunit assignment.
    supported_by: &id001
    - reference_id: PMID:34815343
      supporting_text: 'We elucidated CHX''s mechanism of action based on

        the cryo-electron microscopy structure of actively translating Neurospora crassa

        ribosomes bound with CHX at 2.7-Å resolution.'
    - reference_id: PMID:10852723
      supporting_text: 'Each of the two L39 mutants displayed a

        4-fold increase of their error frequencies over the wild type.'
    - reference_id: file:NEUCR/NCU08990/NCU08990-uniprot.txt
      supporting_text: DR   InterPro; IPR000077; Ribosomal_eL39.
    - reference_id: file:NEUCR/NCU08990/NCU08990-7R81-entity74.json
      supporting_text: '"pdbx_strand_id": "n1" ... "pdbx_description": "60S ribosomal protein L39" ...
        "reference_database_accession": "Q7S2X9"'
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-09
  source_reference_id: file:NEUCR/NCU08990/NCU08990-protnlm-source.json
  predicted_term:
    id: GO:1990904
    label: ribonucleoprotein complex
  predicted_term_type: GO_CC
  review:
    assessment: LSP
    confidence_score: 2
    summary: Neurospora cryo-EM structure 7R81 identifies this exact protein as eL39, entity 74/chain
      VB (author n1), in the translating large ribosomal subunit. The eL39-specific domain agrees with
      the direct structural assignment. GOA already provides cytosolic large ribosomal subunit (GO:0022625),
      a cellular-component descendant of ribonucleoprotein complex. The emitted ribonucleoprotein complex
      prediction is therefore correct but less precise than the established compartment and subunit assignment.
    supported_by: *id001
references:
- id: PMID:34815343
  title: Structure of the translating Neurospora ribosome arrested by cycloheximide.
  full_text_unavailable: true
- id: PMID:10852723
  title: Yeast ribosomal protein L24 affects the kinetics of protein synthesis and ribosomal protein L39
    improves translational accuracy, while mutants lacking both remain viable.
  full_text_unavailable: true
- id: file:NEUCR/NCU08990/NCU08990-uniprot.txt
  title: 'UniProt record for NCU08990: domain and family evidence'
- id: file:NEUCR/NCU08990/NCU08990-protnlm-source.json
  title: Frozen live ProtNLM2 API response for NCU08990
- id: file:NEUCR/NCU08990/NCU08990-7R81-entity74.json
  title: 'RCSB 7R81 polymer entity 74: Neurospora eL39 mapped to Q7S2X9'