id: A7UX10
gene_symbol: NCU11362
locus_tag: NCU11362
taxon:
  id: NCBITaxon:367110
  label: Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
status: COMPLETE
description: NCU11362 is a METTL16-family RNA N6-adenosine methyltransferase inferred to modify U6 snRNA
  using S-adenosylmethionine. The conserved U6 modification promotes accurate pre-mRNA splice-site recognition.
  Nuclear RNA modification is strongly supported by fungal ortholog experiments; a distinct Neurospora
  mRNA methylation repertoire is unresolved.
source_documents:
- genes/NEUCR/NCU11362/NCU11362-protnlm-source.json
- genes/NEUCR/NCU11362/NCU11362-uniprot.txt
- genes/NEUCR/NCU11362/NCU11362-ai-review.yaml
predictions:
- source_method: ProtNLM2
  source_version: UniProt API snapshot 2026-09-09
  source_reference_id: file:NEUCR/NCU11362/NCU11362-protnlm-source.json
  predicted_term:
    id: GO:0044238
    label: primary metabolic process
  predicted_term_type: GO_BP
  review:
    assessment: LSP
    confidence_score: 2
    summary: Specific METTL16 subfamily placement and characterized fungal U6 methylation establish an
      RNA primary-metabolism role. The U6 methyltransferase activity and RNA methylation process are more
      informative than primary metabolic process. RNA methylation (GO:0001510) is a biological-process
      descendant of the emitted term, so LSP reflects a supported difference in process specificity. The
      inference concerns conserved RNA modification, not the bacterial RlmF 23S rRNA substrate.
    supported_by:
    - reference_id: PMID:40841561
      supporting_text: 'The N6-methyladenosine (m6A) modification in U6 snRNA, catalyzed by METTL16

        using S-adenosylmethionine (SAM) as the methyl donor, is required for efficient

        and accurate pre-mRNA splicing.'
    - reference_id: PMID:34050143
      supporting_text: We found that a subset of introns was retained in mRNAs, indicating a splicing
        defect caused by loss of m6A in U6 snRNA.
    - reference_id: file:NEUCR/NCU11362/NCU11362-uniprot.txt
      supporting_text: DR   InterPro; IPR010286; METTL16/RlmF.
    - reference_id: file:NEUCR/NCU11362/NCU11362-uniprot.txt
      supporting_text: DR   PANTHER; PTHR13393:SF0; RNA N6-ADENOSINE-METHYLTRANSFERASE METTL16; 1.
references:
- id: PMID:40841561
  title: Structures and mechanisms of U6 snRNA m(6)A modification by METTL16.
  full_text_unavailable: false
- id: PMID:34050143
  title: A single m(6)A modification in U6 snRNA diversifies exon sequence at the 5' splice site.
  full_text_unavailable: false
- id: file:NEUCR/NCU11362/NCU11362-uniprot.txt
  title: 'UniProt record for NCU11362: domain and family evidence'
- id: file:NEUCR/NCU11362/NCU11362-protnlm-source.json
  title: Frozen live ProtNLM2 API response for NCU11362