KAL-1 is a homeodomain-containing transcriptional regulator in Neurospora crassa. Its conserved homeodomain supports sequence-specific DNA binding and nuclear transcriptional regulation. Deletion of kal-1 alters colony morphology, connecting its regulatory role to fungal growth and differentiation.
Existing Annotations Review
GO Term
Evidence
Action
Reason
GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding
IEA GO_REF:0000117
ACCEPT
Summary: The sequence contains a diagnostic homeodomain (Pfam PF00046; PROSITE domain residues 75β135), supporting sequence-specific DNA binding by a transcriptional regulator.
Reason: The sequence contains a diagnostic homeodomain (Pfam PF00046; PROSITE domain residues 75β135), supporting sequence-specific DNA binding by a transcriptional regulator. The curated phylogenetic inference places its activity in RNA polymerase II regulation; no exact recognition motif or individual target promoter is inferred.
Supporting Evidence:
file:NEUCR/kal-1/kal-1-uniprot.txt
DR Pfam; PF00046; Homeodomain; 1.
GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
IBA GO_REF:0000033
ACCEPT
Summary: The sequence contains a diagnostic homeodomain (Pfam PF00046; PROSITE domain residues 75β135), supporting sequence-specific DNA binding by a transcriptional regulator.
Reason: The sequence contains a diagnostic homeodomain (Pfam PF00046; PROSITE domain residues 75β135), supporting sequence-specific DNA binding by a transcriptional regulator. The curated phylogenetic inference places its activity in RNA polymerase II regulation; no exact recognition motif or individual target promoter is inferred.
Summary: The sequence contains a diagnostic homeodomain (Pfam PF00046; PROSITE domain residues 75β135), supporting sequence-specific DNA binding by a transcriptional regulator.
Reason: The sequence contains a diagnostic homeodomain (Pfam PF00046; PROSITE domain residues 75β135), supporting sequence-specific DNA binding by a transcriptional regulator. The curated phylogenetic inference places its activity in RNA polymerase II regulation; no exact recognition motif or individual target promoter is inferred.
Summary: The sequence contains a diagnostic homeodomain (Pfam PF00046; PROSITE domain residues 75β135), supporting sequence-specific DNA binding by a transcriptional regulator.
Reason: The sequence contains a diagnostic homeodomain (Pfam PF00046; PROSITE domain residues 75β135), supporting sequence-specific DNA binding by a transcriptional regulator. The curated phylogenetic inference places its activity in RNA polymerase II regulation; no exact recognition motif or individual target promoter is inferred.
Summary: The conserved homeodomain and transcriptional regulator placement support nuclear function.
Reason: The conserved homeodomain and transcriptional regulator placement support nuclear function. This is a family-based inference; a direct KAL-1 imaging experiment was not identified.
Supporting Evidence:
file:NEUCR/kal-1/kal-1-uniprot.txt
DR Pfam; PF00046; Homeodomain; 1.
GO:0006355 regulation of DNA-templated transcription
IEA GO_REF:0000002
ACCEPT
Summary: The sequence contains a diagnostic homeodomain (Pfam PF00046; PROSITE domain residues 75β135), supporting sequence-specific DNA binding by a transcriptional regulator.
Reason: The sequence contains a diagnostic homeodomain (Pfam PF00046; PROSITE domain residues 75β135), supporting sequence-specific DNA binding by a transcriptional regulator. The curated phylogenetic inference places its activity in RNA polymerase II regulation; no exact recognition motif or individual target promoter is inferred.
Supporting Evidence:
file:NEUCR/kal-1/kal-1-uniprot.txt
DR Pfam; PF00046; Homeodomain; 1.
GO:0006357 regulation of transcription by RNA polymerase II
IBA GO_REF:0000033
ACCEPT
Summary: The sequence contains a diagnostic homeodomain (Pfam PF00046; PROSITE domain residues 75β135), supporting sequence-specific DNA binding by a transcriptional regulator.
Reason: The sequence contains a diagnostic homeodomain (Pfam PF00046; PROSITE domain residues 75β135), supporting sequence-specific DNA binding by a transcriptional regulator. The curated phylogenetic inference places its activity in RNA polymerase II regulation; no exact recognition motif or individual target promoter is inferred.
Summary: The curated differentiation inference is consistent with the kal-1 deletion phenotype affecting colony morphology in Figure 3 of the Neurospora transcription-factor knockout study.
Reason: The curated differentiation inference is consistent with the kal-1 deletion phenotype affecting colony morphology in Figure 3 of the Neurospora transcription-factor knockout study. It describes a developmental output of the transcription factor rather than its DNA-binding mechanism.
Supporting Evidence:
file:NEUCR/kal-1/kal-1-uniprot.txt
DR Pfam; PF00046; Homeodomain; 1.
file:NEUCR/kal-1/kal-1-notes.md
Colony morphology of wild type and Ξkal-1:NCU03593.
Core Functions
KAL-1 is a homeodomain-containing transcriptional regulator in Neurospora crassa.
These computational predictions are reviewed separately from the GOA annotation set used for this review. The assessments below are from this project and do not constitute official GO annotations or endorsement by GO/UniProt. They are not included in the existing annotation review above.
Review rationale: A conserved homeodomain is explicitly assigned to KAL-1 by Pfam and PROSITE, providing sequence evidence for DNA binding. The curated phylogenetic annotation specifies RNA polymerase II cis-regulatory region sequence-specific DNA binding. Generic DNA binding is consequently supported but less precise than that existing annotation. No particular DNA sequence preference is assigned here.