mcr-1

Summary: PTN000452207 supports a broad cytochrome-b5 reductase activity at a mixed MCR1/PGA3 eukaryotic node, but the NADH-specific child is already captured by the target's Rhea/EC row.

Reason: The PTN000452207 molecular-function assertion is seeded by S. cerevisiae MCR1 and PGA3 at a broad eukaryotic node, so the IBA is not a failed MCR1-specific inference. It can support only the NAD(P)H-dependent cytochrome-b5 reductase parent, while Neurospora mcr-1 is separately mapped to EC 1.6.2.2 and the NADH-dependent Rhea reaction used for GO:0090524; the broad GO:0004128 claim is therefore true but entailed by the accepted NADH-specific child term for this target.

Summary: PTN000452207 places the MCR1-like subfamily in mitochondria and is consistent with the characterized mitochondrial S. cerevisiae Mcr1 ortholog.

Reason: N. crassa mcr-1 sits in PTHR19370:SF171 with S. cerevisiae MCR1, whose product is experimentally sorted to two submitochondrial compartments; PAINT placed the mitochondrion assertion at a eukaryotic node seeded by S. cerevisiae MCR1 and two T. brucei cytochrome-b5 reductase-like proteins. The inferred mitochondrial outer-membrane localization on the N. crassa UniProt record makes mitochondrion the right activity-site granularity for this IBA.

Summary: The UniProt subcellular-location transfer to the mitochondrial outer membrane matches the N-terminally anchored mcr-1 family member.

Reason: The N. crassa Swiss-Prot record infers a single-pass mitochondrial outer-membrane protein, and the S. cerevisiae ortholog has an experimentally characterized outer-membrane isoform. This is the most specific localization currently available for Neurospora mcr-1.

Summary: PTN000452208 propagates an inherited S. cerevisiae MCR1 ergosterol-biosynthesis assertion, but no Neurospora evidence currently makes the process a core mcr-1 function.

Reason: The PAINT assertion comes from an S. cerevisiae MCR1 seed and N. crassa mcr-1 is in the same MCR1-like branch, so the IBA is retained as an in-clade inherited process annotation. It is not promoted to a core Neurospora mcr-1 function here because mcr-1 has not been assayed in Neurospora, and the available CYP51 reconstitution evidence used the microsomal cytochrome-b5/NADH-cytochrome-b5-reductase system that corresponds to the ER CBR1 paralog branch rather than this mitochondrial MCR1 branch.

Summary: InterPro correctly detects a pyridine nucleotide cytochrome reductase, but its oxidoreductase parent is less informative than the Rhea-supported cytochrome-b5 reductase child.

Reason: mcr-1 has the conserved CBR-like domains and its UniProt record already maps the protein to the NADH:cytochrome-b5 oxidoreductase reaction. The broad GO:0016491 parent loses the electron donor and acceptor specificity and is entailed by GO:0090524 on this protein.

Summary: The Rhea/EC mapping captures the expected NADH-dependent cytochrome-b5 reductase activity for Neurospora mcr-1.

Reason: EC 1.6.2.2 and RHEA:46680 specify reduction of ferricytochrome b5 using NADH. This is the precise molecular-function term for mcr-1's conserved cytochrome-b5 reductase domains and subsumes the broader GO:0004128 and GO:0016491 parent terms that are also present in GOA.