wc-1 (white collar-1) encodes the principal blue-light photoreceptor of Neurospora crassa: a GATA-type zinc-finger transcription factor with a light-sensing LOV (PAS) domain. With WC-2 it forms the White Collar Complex (WCC), the master activator of light-induced transcription that drives photoadaptation, entrainment of the circadian clock (activating frq), and light/clock gating of conidiation.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0009882 blue light photoreceptor activity | IDA PMID:20813262 Photoadaptation in Neurospora by competitive interaction of ... | NEW | Summary: Proposed new annotation. WC-1 is the LOV-domain photosensor of the WCC; this molecular function is absent from GOA despite being the gene's defining activity. Reason: The WCC's light response is mediated by its LOV domains, and WC-1 is the LOV-containing subunit; light triggers LOV-dependent WCC dimerization. GOA carries only the downstream transcription terms, leaving the photoreceptor activity itself unannotated. Supporting Evidence: PMID:20813262 Light responses and photoadaptation of Neurospora depend on the photosensory light-oxygen-voltage (LOV) domains of the circadian transcription factor White Collar Complex (WCC) and its negative regulator VIVID (VVD). PMID:20813262 We found that light triggers LOV-mediated dimerization of the WCC. PMID:20733070 the white collar complex (WCC), the primary blue-light photoreceptor and the transcription factor complex that initiates light-regulated transcriptional responses in Neurospora |
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: Accept: nucleus. Reason: The White Collar Complex acts on target promoters in the nucleus, and is_active_in is the right qualifier for the site of WC-1's transcriptional activity. |
| GO:0005634 nucleus | IEA GO_REF:0000044 | ACCEPT | Summary: Accept: nucleus. Reason: Electronic route to the same nuclear location as the IBA row; redundant with it. |
| GO:0006355 regulation of DNA-templated transcription | IEA GO_REF:0000002 | ACCEPT | Summary: Accept: regulation of DNA-templated transcription. Reason: Generic parent. WC-1's characterised role is light-driven activation of clock and photoresponse genes as part of the WCC. |
| GO:0008270 zinc ion binding | IEA GO_REF:0000002 | ACCEPT | Summary: Accept: zinc ion binding. Reason: Zinc is structural to the GATA-type finger that mediates WC-1's DNA binding; a cofactor requirement rather than a separate function. |
| GO:0043565 sequence-specific DNA binding | IEA GO_REF:0000002 | ACCEPT | Summary: Accept: sequence-specific DNA binding. Reason: The GATA finger binds specific promoter elements, so this is more informative than bare DNA binding. The LOV-domain photoreceptor activity that distinguishes WC-1 from WC-2 is annotated separately in this review. |
| GO:0005515 protein binding | IPI PMID:19427309 Activity of the circadian transcription factor White Collar ... | KEEP AS NON CORE | Summary: Protein binding (uninformative MF) reflecting White Collar Complex / clock interactions. Reason: Interaction evidence from the study of WCC phosphorylation at SP motifs, i.e. WC-1 with its complex partner WC-2 (P78714). Real, but 'protein binding' names no function; the informative statement is WCC membership, for which GO currently has no specific complex term. |
| GO:0005515 protein binding | IPI PMID:20733070 Physical interaction between VIVID and white collar complex ... | KEEP AS NON CORE | Summary: Protein binding (uninformative MF) reflecting White Collar Complex / clock interactions. Reason: This row's WITH/FROM partner is WC-2 (P78714), i.e. the WC-1/WC-2 association within the White Collar Complex detected in this co-IP study of the VIVID-WCC interaction. Kept as non-core because the term records that a partner exists without saying what WC-1 does; the informative statement is WCC membership. |
| GO:0005515 protein binding | IPI PMID:20807745 VIVID interacts with the WHITE COLLAR complex and FREQUENCY-... | KEEP AS NON CORE | Summary: Protein binding (uninformative MF) reflecting White Collar Complex / clock interactions. Reason: This row's WITH/FROM partner is WC-2 (P78714), reflecting the WC-1/WC-2 heterodimer (White Collar Complex) assayed alongside VIVID in this study; again a partner list rather than a molecular function. |
| GO:0005515 protein binding | IPI PMID:20813262 Photoadaptation in Neurospora by competitive interaction of ... | KEEP AS NON CORE | Summary: Protein binding (uninformative MF) reflecting White Collar Complex / clock interactions. Reason: Interaction evidence from the competitive LOV-domain model of photoadaptation, where VIVID competes with WC-1's own LOV domain. The mechanistic content sits in the photoreceptor annotation, not in this bare binding term. |
| GO:0005515 protein binding | IPI PMID:20733070 Physical interaction between VIVID and white collar complex ... | KEEP AS NON CORE | Summary: Protein binding with VIVID (VVD, Q9C3Y6), detected by co-IP after cross-linking; the VVD-WCC interaction underlies photoadaptation. Reason: The VVD-WC-1 interaction is well supported experimentally and is biologically meaningful (VVD represses WCC activity in constant light), but 'protein binding' names no activity of WC-1. The mechanistic content is captured by the photoreceptor and transcription annotations; this is retained as non-core interaction evidence. Supporting Evidence: PMID:20733070 Through co-IP analysis after protein cross-linking and live-cell imaging of GFP-VVD, we find a specific physical interaction between VVD and WCC and, additionally, that both are present in the nucleus on light induction. |
| GO:0005515 protein binding | IPI PMID:20807745 VIVID interacts with the WHITE COLLAR complex and FREQUENCY-... | KEEP AS NON CORE | Summary: Protein binding with VIVID (VVD, Q9C3Y6); VVD binds WC-1 in constant light and after transfer to darkness. Reason: Independent confirmation of the VVD-WC-1 interaction through which VVD inhibits WCC transcriptional activity. Real but uninformative as a molecular function of WC-1; kept as non-core. Supporting Evidence: PMID:20807745 We have shown here that VVD binds WC-1 both in LL and upon release into DD. |
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