NaA622_candidate_IFRH_0

UniProt ID: A0A1J6I5H4
Organism: Nicotiana attenuata
Review Status: DRAFT
Aliases:
IFRH_0 NaA622
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Gene Description

NaA622_candidate_IFRH_0 is an alternate A622-like oxidoreductase paralog in Nicotiana attenuata. Current evidence makes it a live comparison candidate for the A622/NaGR step, but the paper-backed mechanistic assignment is stronger for the primary NaA622 mapping than for this alternate accession.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0016491 oxidoreductase activity
IEA
GO_REF:0000002 		ACCEPT
Summary: Generic oxidoreductase activity is acceptable for this A622-like paralog, but it does not resolve whether IFRH_0 is the true late nicotine-pathway copy.
Reason: The accession clearly belongs to the oxidoreductase-like A622 family, even though its exact pathway placement relative to the primary A622 mapping is still unresolved.
Supporting Evidence:
file:NICAT/NaA622_candidate_IFRH_0/NaA622_candidate_IFRH_0-notes.md
The full preprint makes A622/NaGR one of the mechanistically resolved enzymes in nicotine synthesis, so IFRH_0 remains an A622-like paralog that has to be compared explicitly against the primary A622 mapping rather than dropped as noise.

Core Functions

IFRH_0 is an alternate A622-like oxidoreductase paralog that should be compared directly against the primary NaA622 mapping for late nicotine-pathway reductase activity.

Molecular Function:
oxidoreductase activity
Supporting Evidence:
  • file:NICAT/NaA622_candidate_IFRH_0/NaA622_candidate_IFRH_0-notes.md
    Because the paper is strongest for pathway role and weaker for exact NICAT accession resolution, IFRH_0 remains a live alternate A622-like candidate until the paralog split is settled directly.

References

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Suggested Questions for Experts

Q: Does IFRH_0 have measurable nicotinic acid N-glucoside reductase activity, or is that chemistry restricted to the primary A622 mapping?

Q: Are IFRH_0 and IFRH_2 differentially expressed across root cell types or induction conditions relevant to nicotine production?

Suggested Experiments

Experiment: Test recombinant IFRH_0 for NaGR-like activity using nicotinic acid N-glucoside as substrate and compare directly with the primary A622 accession.

Hypothesis: IFRH_0 has weaker or no NaGR activity relative to the primary A622 mapping.

Type: biochemical enzyme assay

Experiment: Quantify root expression and metabolite consequences after selective disruption of IFRH_0 versus the primary A622 paralog.

Hypothesis: IFRH_0 contributes less to late nicotine flux than the primary NaA622 mapping.

Type: comparative genetics plus metabolite profiling

Deep Research

OpenAI

(NaA622_candidate_IFRH_0-deep-research-openai.md)

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📚 Additional Documentation

Notes

(NaA622_candidate_IFRH_0-notes.md)

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