NaBBL2_candidate_FOX1_2 is a BBL-like oxidoreductase candidate for the late nicotine pathway in Nicotiana attenuata. BBL-family participation in the stereoselective late pathway is strongly supported, and FOX1_2 remains a leading candidate for the attenuata BBL2-like copy.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005773 vacuole | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Vacuolar localization is plausible and worth retaining as non-core context. Reason: UniProt supports a vacuolar location, but the key curation signal is the catalytic late-pathway role rather than compartment detail. Supporting Evidence: file:NICAT/NaBBL2_candidate_FOX1_2/NaBBL2_candidate_FOX1_2-uniprot.txt CC -!- SUBCELLULAR LOCATION: Vacuole {ECO:0000256|ARBA:ARBA00004116}. |
| GO:0016491 oxidoreductase activity | IEA GO_REF:0000002 | MARK AS OVER ANNOTATED | Summary: This generic oxidoreductase term is true but underspecific. Reason: The BBL family is now tied to a specific late nicotine oxidase role, so the parent term is not the most informative curation outcome. Supporting Evidence: file:NICAT/NaBBL2_candidate_FOX1_2/NaBBL2_candidate_FOX1_2-notes.md The full preprint makes BBLa/NicGS a mechanistically defined late-pathway enzyme that drives stereoselective nicotine formation, with both structural and dropout support. |
| GO:0050660 flavin adenine dinucleotide binding | IEA GO_REF:0000002 | ACCEPT | Summary: FAD binding is an appropriate cofactor annotation for FOX1_2. Reason: BBL-family proteins are FAD-linked oxidoreductases, and this cofactor term is informative and specific. |
| GO:0071949 FAD binding | IEA GO_REF:0000002 | REMOVE | Summary: This is redundant with GO:0050660. Reason: Keep GO:0050660 as the preferred cofactor-binding annotation and drop the duplicate variant. |
| GO:0042179 nicotine biosynthetic process | IEA GO_REF:0000041 | ACCEPT | Summary: This is an appropriate core pathway annotation for FOX1_2. Reason: FOX1_2 is the best current attenuata BBL2-like anchor, and the BBL family is now directly supported as a core late nicotine-pathway module. Supporting Evidence: file:NICAT/NaBBL2_candidate_FOX1_2/NaBBL2_candidate_FOX1_2-notes.md That strengthens the existing choice to keep FOX1_2 as the best current BBL2-like anchor, while still leaving exact NICAT BBL paralog resolution open. |
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Download this section (compressed HTML)Q: Does FOX1_2 perform the same substrate-specific NicGS chemistry inferred for the tobacco BBL ortholog?
Q: Are the remaining BBL-family paralogs partially redundant with FOX1_2 or functionally divergent?
Experiment: Reconstitute the late nicotine pathway with FOX1_2 and assay stereoselective formation of nicotine glucoside and nicotine.
Hypothesis: FOX1_2 is the principal attenuata BBL/NicGS paralog.
Type: pathway reconstitution assay
Experiment: Profile nicotine stereochemistry and glucosylated intermediates after targeted FOX1_2 disruption.
Hypothesis: Loss of FOX1_2 will substantially impair the stereoselective late nicotine step.
Type: genetics plus chiral metabolite profiling
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