NaBGL1_candidate_BGLU42

Gene Description

NaBGL1_candidate_BGLU42 is a lower-confidence historical GH1 beta-glucosidase comparator that was launched before the current mapping pass. The pathway paper secures beta-GD/NicGH biology at the family level, but the newer sequence-backed mapping now favors the BGLU18 pair rather than BGLU42 as the best current NICAT orthology anchors to the nicotine-pathway hydrolases.

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds	IEA GO_REF:0000002	MODIFY	Summary: This parent hydrolase term should be replaced by the more specific beta-glucosidase term. Reason: GO:0008422 already captures the relevant glycosidase specificity for this GH1 family enzyme. Proposed replacements: beta-glucosidase activity
GO:0005975 carbohydrate metabolic process	IEA GO_REF:0000002	MARK AS OVER ANNOTATED	Summary: This broad process term is true in a generic sense but not the informative curation takeaway. Reason: The evidence that remains secure for this accession is generic GH1 beta-glucosidase chemistry rather than a specific pathway assignment.
GO:0008422 beta-glucosidase activity	IEA GO_REF:0000120	ACCEPT	Summary: This is an appropriate core molecular-function annotation. Reason: UniProt identifies terminal beta-D-glucoside hydrolysis chemistry for this GH1 enzyme. Supporting Evidence: file:NICAT/NaBGL1_candidate_BGLU42/NaBGL1_candidate_BGLU42-uniprot.txt Reaction=Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.; EC=3.2.1.21;
GO:0009821 alkaloid biosynthetic process	IEA GO_REF:0000117	REMOVE	Summary: This pathway-process assignment is no longer strong enough for this specific accession. Reason: The current mapping work indicates that BGLU42 is not the best orthology anchor to the specialized beta-GD pathway enzymes.
GO:0030245 cellulose catabolic process	IEA GO_REF:0000002	REMOVE	Summary: This appears to be an over-transfer from the broad GH1 family and should be removed. Reason: This appears to be a generic GH1 family overcall rather than a reviewed biological role for BGLU42.

Core Functions

BGLU42 is a GH1 beta-glucosidase retained as a lower-confidence comparator while the BGLU18_6 and BGLU18_1 candidates are now preferred as the main nicotine-pathway orthology anchors.

Molecular Function:

beta-glucosidase activity

Directly Involved In:

carbohydrate metabolic process

Supporting Evidence:

file:NICAT/NaBGL1_candidate_BGLU42/NaBGL1_candidate_BGLU42-notes.md
The 2026-04-05 mapping dive now favors BGLU18_6 and BGLU18_1 as the best sequence-backed NICAT orthology anchors to tobacco beta-GD1 and beta-GD2, so BGLU42 should be retained only as a lower-confidence historical comparator.

References

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Suggested Experiments

Experiment: Compare BGLU42 directly against BGLU18_6 and BGLU18_1 using nicotine-pathway glucosides and generic GH1 substrates.

Hypothesis: BGLU42 will show a substrate profile distinct from the preferred BGLU18 pathway candidates.

Type: biochemical substrate-specificity assay

Experiment: Disrupt BGLU42 alone and in combination with the BGLU18 candidates to test for any hidden redundancy in nicotine accumulation phenotypes.

Hypothesis: BGLU42 loss alone will have weaker nicotine-pathway effects than disruption of the BGLU18 candidates.

Type: genetic perturbation plus metabolite profiling

Gene Description

Existing Annotations Review

Core Functions

BGLU42 is a GH1 beta-glucosidase retained as a lower-confidence comparator while the BGLU18_6 and BGLU18_1 candidates are now preferred as the main nicotine-pathway orthology anchors.

References

Suggested Questions for Experts

Suggested Experiments

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📚 Additional Documentation

Notes

📄 View Raw YAML