NaBGL2_candidate_BGLU18_1

Gene Description

NaBGL2_candidate_BGLU18_1 is the best current NICAT mapping for the beta-GD2-like homeologous late hydrolase in the nicotine pathway. The evidence is somewhat less direct than for the beta-GD1 anchor, but the pathway paper and mapping pass together keep BGLU18_1 as the leading attenuata candidate for the second beta-GD-like copy.

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds	IEA GO_REF:0000120	MODIFY	Summary: This broad hydrolase term should be narrowed to beta-glucosidase activity. Reason: GO:0008422 is the more informative GH1 activity term for this candidate. Proposed replacements: beta-glucosidase activity
GO:0005975 carbohydrate metabolic process	IEA GO_REF:0000002	MARK AS OVER ANNOTATED	Summary: This generic process term is too broad for the pathway-focused review. Reason: The important curation question is whether this copy participates in late nicotine-pathway glucoside hydrolysis, not generic carbohydrate metabolism.
GO:0008422 beta-glucosidase activity	IEA GO_REF:0000118	ACCEPT	Summary: Beta-glucosidase activity is the appropriate core molecular-function annotation. Reason: UniProt and GH1 family assignment support beta-glucosidase chemistry for this beta-GD2-like candidate. Supporting Evidence: file:NICAT/NaBGL2_candidate_BGLU18_1/NaBGL2_candidate_BGLU18_1-uniprot.txt DR GO; GO:0008422; F:beta-glucosidase activity; IEA:TreeGrafter.
GO:0042179 nicotine biosynthetic process	TAS file:NICAT/NaBGL2_candidate_BGLU18_1/NaBGL2_candidate_BGLU18_1-notes.md	NEW	Summary: BGLU18_1 should be added as a plausible homeologous nicotine-pathway hydrolase candidate. Reason: The pathway paper retains a beta-GD2-like homeologous cluster, and the mapping pass now identifies BGLU18_1 as the best current attenuata ortholog. Supporting Evidence: file:NICAT/NaBGL2_candidate_BGLU18_1/NaBGL2_candidate_BGLU18_1-notes.md The 2026-04-05 mapping dive assigns NaBGL2 to BGLU18_1 / A0A314KWB2 as the best current sequence-backed NICAT ortholog to tobacco beta-GD2.

Core Functions

BGLU18_1 is the leading current NICAT candidate for the homeologous beta-GD2-like late hydrolase branch in nicotine biosynthesis.

Molecular Function:

beta-glucosidase activity

Directly Involved In:

nicotine biosynthetic process

Supporting Evidence:

file:NICAT/NaBGL2_candidate_BGLU18_1/NaBGL2_candidate_BGLU18_1-notes.md
The glucosylation preprint identifies a homeologous A622L-MATE2-beta-GD2 cluster alongside A622-MATE1-beta-GD1, which keeps a second beta-GD-like hydrolase in the pathway frame even though beta-GD1 is the more directly assayed late-step anchor.

References

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Suggested Experiments

Experiment: Compare BGLU18_1 and BGLU18_6 side by side in late-pathway glucoside hydrolysis assays.

Hypothesis: BGLU18_1 is catalytically competent but weaker or differently tuned than the primary BGLU18_6 anchor.

Type: comparative biochemical assay

Experiment: Generate single and double disruptions of the two BGLU18 candidates and quantify glucosylated late intermediates and nicotine accumulation.

Hypothesis: The two BGLU18 copies have overlapping but non-identical contributions to late nicotine-pathway flux.

Type: genetics plus metabolite profiling

Gene Description

Existing Annotations Review

Core Functions

BGLU18_1 is the leading current NICAT candidate for the homeologous beta-GD2-like late hydrolase branch in nicotine biosynthesis.

References

Suggested Questions for Experts

Suggested Experiments

Deep Research

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📚 Additional Documentation

Notes

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