NaMATE1_candidate_DTX40_3 is the best current NICAT mapping for the MATE1-like transporter genetically linked to the late nicotine-pathway module. The public record still describes a generic MATE detoxification transporter, but the pathway paper and mapping pass together support this accession as the leading attenuata candidate for the pathway-associated MATE transporter.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0015297 antiporter activity | IEA GO_REF:0000002 | ACCEPT | Summary: Antiporter activity is an appropriate catalytic-family annotation for this MATE candidate. Reason: The current public evidence clearly supports a MATE-family antiporter even though the exact specialized substrate remains unresolved. |
| GO:0016020 membrane | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: Membrane localization is appropriate but secondary to the pathway interpretation. Reason: This is a multi-pass membrane transporter, but the central review issue is its pathway placement rather than generic localization. Supporting Evidence: file:NICAT/NaMATE1_candidate_DTX40_3/NaMATE1_candidate_DTX40_3-uniprot.txt CC -!- SUBCELLULAR LOCATION: Membrane |
| GO:0042910 xenobiotic transmembrane transporter activity | IEA GO_REF:0000002 | UNDECIDED | Summary: The xenobiotic-transport assignment remains unresolved until the transported substrate is tested. Reason: Current evidence points toward a specialized nicotine-module transporter role, but the exact transported metabolite remains experimentally unresolved. Because MATE transporters often move alkaloids and other secondary metabolites, the nicotine-pathway placement does not by itself falsify a xenobiotic transmembrane transporter annotation. Supporting Evidence: file:NICAT/NaMATE1_candidate_DTX40_3/NaMATE1_candidate_DTX40_3-deep-research-falcon.md No primary publication directly characterizes A0A314KVN4 / DTX40_3; annotation rests on MATE family inference (multidrug and toxic compound extrusion antiporters that often transport plant secondary metabolites) plus the nicotine-pathway genomic and co-expression evidence. |
| GO:0055085 transmembrane transport | IEA GO_REF:0000002 | KEEP AS NON CORE | Summary: This broad process term is reasonable context but not the key curation outcome. Reason: Keep the transport process annotation while prioritizing the antiporter function and pathway-specific interpretation. |
| GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane | IEA GO_REF:0000002 | UNDECIDED | Summary: This detoxification-process annotation is unresolved without substrate and localization evidence. Reason: The reviewed evidence supports a pathway-associated metabolite transporter, but it does not identify the transported substrate or establish whether export across the plasma membrane is the relevant compartment. A nicotine- related substrate could still fit a xenobiotic-detoxification framing, so this process annotation should remain undecided pending direct transport and localization assays. Supporting Evidence: file:NICAT/NaMATE1_candidate_DTX40_3/NaMATE1_candidate_DTX40_3-deep-research-falcon.md The exact transported metabolite (nicotine, nicotine glucoside, or another intermediate) and the precise membrane localization remain experimentally unresolved. |
| GO:0042179 nicotine biosynthetic process | TAS file:NICAT/NaMATE1_candidate_DTX40_3/NaMATE1_candidate_DTX40_3-notes.md | NEW | Summary: MATE1 should be added as a pathway-associated nicotine biosynthetic gene. Reason: The pathway paper keeps MATE1 in the core late module on the basis of gene clustering and coordinated expression, even though its exact transported substrate remains to be nailed down experimentally. Supporting Evidence: file:NICAT/NaMATE1_candidate_DTX40_3/NaMATE1_candidate_DTX40_3-notes.md The glucosylation preprint identifies an A622-MATE1-beta-GD1 cluster, reports that these genes are root enriched and tightly co-expressed with nicotine biosynthesis genes, and keeps MATE1 in the core late-pathway module even though the exact transported metabolite remains unresolved. |
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Download this section (compressed HTML)Q: What is the direct transported substrate of the attenuata MATE1 candidate: nicotine glucoside, nicotine, or another late-pathway intermediate?
Q: Is DTX40_3 localized to the vacuolar membrane, plasma membrane, or another endomembrane compartment in the nicotine pathway context?
Experiment: Express DTX40_3 in a heterologous transport system and test transport of nicotine, nicotine glucoside, and related late-pathway intermediates.
Hypothesis: DTX40_3 preferentially transports one or more late nicotine-pathway metabolites, and direct assays are needed to determine whether xenobiotic transport annotations also apply.
Type: transporter substrate-specificity assay
Experiment: Disrupt the primary MATE1 candidate and profile subcellular metabolite partitioning together with total nicotine output after induction.
Hypothesis: Loss of DTX40_3 will alter late-pathway metabolite partitioning and reduce efficient nicotine accumulation.
Type: genetics plus compartment-resolved metabolite profiling
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