| Claim/Aspect | Key evidence/details | Source | DOI/URL | Pub date |
|---|---|---|---|---|
| Identity / disambiguation | Rice **OsLSD1** is a plant-specific small zinc-finger protein, not the mammalian LSD1/KDM1A demethylase. Cloned as **GenBank AY525368** from *Oryza sativa*; cDNA **988 bp**, ORF **432 bp**, predicted protein **143 aa** (~**14.8 kDa**), mapped to **chromosome 8** (PAC clone P0672D01). Rice literature also links OsLSD1 to **LOC_Os08g06280** and review/table evidence links the family entry to **Os08g0159500**. (pqac-00000000, pqac-00000005, pqac-00000006, pqac-00000009, pqac-00000011, pqac-00000012) | Wang 2005, *Molecular Plant-Microbe Interactions*; Li 2014, *POJ*; Kang 2021, *Plants* | https://doi.org/10.1094/mpmi-18-0375 ; https://doi.org/10.3316/informit.897038318452468 ; https://doi.org/10.3390/plants10081598 | May 2005; 2014; Aug 2021 |
| Domains / family features | OsLSD1 contains **three internally conserved C2C2-type zinc finger / LSD1-like domains** with consensus motif reported as **CxxCxxLLMYxxGAxSVxCxxC**; it shows **58% identity** to Arabidopsis LSD1 and **85% identity** to Arabidopsis LOL1. Rice lesion-mimic review classifies it as a **C2C2-type zinc finger protein**. (pqac-00000001, pqac-00000005, pqac-00000013, pqac-00000014) | Wang 2005, *Molecular Plant-Microbe Interactions*; Kang 2021, *Plants* | https://doi.org/10.1094/mpmi-18-0375 ; https://doi.org/10.3390/plants10081598 | May 2005; Aug 2021 |
| Subcellular localization | **OsLSD1-GFP localized to the nucleus** in tobacco cells/root tips, supporting nuclear function. A later metacaspase study notes OsMC1–OsLSD1 interaction may occur in the nucleus and cites nucleus-localized LSD1 homolog evidence. (pqac-00000001, pqac-00000005, pqac-00000008, pqac-00000013, pqac-00000015) | Wang 2005, *Molecular Plant-Microbe Interactions*; Huang 2015, *International Journal of Molecular Sciences* | https://doi.org/10.1094/mpmi-18-0375 ; https://doi.org/10.3390/ijms160716216 | May 2005; Jul 2015 |
| Function in programmed cell death (PCD) | Core interpretation: OsLSD1 is a **negative regulator of plant PCD**. **Antisense** suppression produced **lesion mimic phenotype**, increased **PR-1 mRNA**, and **accelerated hypersensitive response** to avirulent blast isolates; overexpression in tobacco enhanced tolerance to the PCD-inducing toxin **fumonisin B1 (FB1)**. Reviews summarize OsLSD1 as regulating **PCD and hypersensitive response**. (pqac-00000005, pqac-00000006, pqac-00000007, pqac-00000010, pqac-00000013, pqac-00000014, pqac-00000018) | Wang 2005, *Molecular Plant-Microbe Interactions*; Huang 2015, *International Journal of Molecular Sciences*; Kang 2021, *Plants* | https://doi.org/10.1094/mpmi-18-0375 ; https://doi.org/10.3390/ijms160716216 ; https://doi.org/10.3390/plants10081598 | May 2005; Jul 2015; Aug 2021 |
| Callus differentiation / regeneration | Overexpression of OsLSD1 **accelerated callus differentiation** and plant regeneration. In transformed rice calli, differentiation time decreased from **7–10 days** in vector controls to **3–5 days** in OsLSD1-overexpression lines. Authors conclude OsLSD1 plays a **positive role in callus differentiation**. (pqac-00000005, pqac-00000006, pqac-00000012, pqac-00000018) | Wang 2005, *Molecular Plant-Microbe Interactions* | https://doi.org/10.1094/mpmi-18-0375 | May 2005 |
| Disease resistance / blast interaction | OsLSD1 is linked to rice blast defense. In Wang et al., both **sense and antisense** transgenics showed **significantly enhanced resistance** to a virulent blast isolate; antisense plants also showed faster defense/HR responses to avirulent blast. Expression/network analysis later listed **OsLSD1 (LOC_Os08g06280)** among rice ZFP genes associated with defense against *Magnaporthe oryzae*, and reported the **LSD1 family was repressed** after inoculation in their expression dataset. (pqac-00000005, pqac-00000006, pqac-00000007, pqac-00000011, pqac-00000016, pqac-00000018) | Wang 2005, *Molecular Plant-Microbe Interactions*; Li 2014, *POJ* | https://doi.org/10.1094/mpmi-18-0375 ; https://doi.org/10.3316/informit.897038318452468 | May 2005; 2014 |
| Interaction partners | Yeast two-hybrid assays showed **OsMC1, OsMC2, and OsMC3 interact with OsLSD1 and OsLSD3**, but not with OsLSD2/OsLOL1/OsLOL2; **OsMC3 only interacted with OsLSD1** in one summary. N- and C-terminal regions of **OsMC1** also interacted with OsLSD1. These data place OsLSD1 in metacaspase-linked PCD signaling. (pqac-00000008, pqac-00000010, pqac-00000015) | Huang 2015, *International Journal of Molecular Sciences* | https://doi.org/10.3390/ijms160716216 | Jul 2015 |
| Expression regulation | OsLSD1 transcript abundance is **light-induced / dark-suppressed**: after **24 h dark** treatment, transcript levels decreased markedly by **4 h** and **24 h** in darkness, then recovered after re-exposure to light (**4–5 h**). RT-PCR detected constitutive expression in **root, stem, and leaf**. (pqac-00000005, pqac-00000006, pqac-00000012, pqac-00000013, pqac-00000018) | Wang 2005, *Molecular Plant-Microbe Interactions* | https://doi.org/10.1094/mpmi-18-0375 | May 2005 |
| Recent GWAS association (2024) | A 2024 rice GWAS for **callus induction rate (CIR)** used **368 accessions** and **994,188 SNPs**, identifying **104 significant SNPs** and **13 candidate genes**. **OsLSD1** was nominated as a candidate based on annotation/transcriptome overlap, reinforcing prior evidence that OsLSD1 contributes to callus differentiation, although this study did **not** functionally validate OsLSD1 directly. (pqac-00000017) | Song 2024, *Rice* | https://doi.org/10.1186/s12284-024-00742-8 | Oct 2024 |


*Table: This table compiles key evidence for rice OsLSD1/Protein LSD1 (UniProt Q0J7V9; LOC_Os08g06280; Os08g0159500), emphasizing identity verification, molecular features, biological roles, and recent 2024 genetic association data. It is useful as a compact evidence map linking specific claims to primary and review sources.*