OsAUX1 (Os01g0856500/LOC_Os01g63770) is a rice plasma-membrane auxin influx carrier of the AUX/LAX branch of the amino-acid/auxin permease superfamily. Native loss-of-function and locus-matched complementation of Arabidopsis aux1 support its role in moving auxin into cells. It contributes to lateral-root initiation, root gravitropic growth and the root-hair response to low phosphate; its root-hair expression pattern differs from Arabidopsis AUX1. Altered cadmium sensitivity reflects auxin and stress physiology and does not establish cadmium transport.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003333 amino acid transmembrane transport | IBA GO_REF:0000033 | UNDECIDED | Summary: Amino-acid transport is unresolved separately from the supported auxin-influx function. Reason: The PAINT IBD at PTN001118955 in PTHR48017 is grounded in amino-acid transporter descendants, not merely sequence similarity. The inspected table also contains a negative IRD at PTN004585555, but target descent from that pruned node has not been established and is not assumed. Target-specific native genetics and complementation support auxin influx (PMID:29650967); they neither demonstrate nor exclude amino-acid substrates. No assay of amino-acid transport or target-specific loss was recovered. Retain explicit uncertainty rather than calling auxin specificity proof of an erroneous IBA. Propagation Review Root cause: UNRESOLVED Sources checked: PANTHER:PTN001118955 UNRESOLVED Amino-acid IBD inspected in PTHR48017 PAINT table. Target substrate range and relationship to the separately recorded IRD node remain unresolved. Supporting Evidence: PMID:29650967 This genetic assay revealed that only one of the OsAUX1 sequences (Os01g63770) was able to successfully rescue the aux1 mutant’s root agravitropic defect (Supplementary Figure 1b, c). |
| GO:0005886 plasma membrane | IEA GO_REF:0000044 | ACCEPT | Summary: OsAUX1 localizes at the plasma membrane. Reason: The native rice study explicitly reports PM localization (PMID:26140668); it independently supports the curated location inference. Its root-hair expression differs from Arabidopsis AUX1, so tissue expression is not transferred wholesale. Supporting Evidence: PMID:26140668 However, OsAUX1, like AtAUX1, localizes at the plasma membrane and appears to function as an auxin tranporter. |
| GO:0015171 amino acid transmembrane transporter activity | IBA GO_REF:0000033 | UNDECIDED | Summary: Amino-acid transport is unresolved separately from the supported auxin-influx function. Reason: The PAINT IBD at PTN001118955 in PTHR48017 is grounded in amino-acid transporter descendants, not merely sequence similarity. The inspected table also contains a negative IRD at PTN004585555, but target descent from that pruned node has not been established and is not assumed. Target-specific native genetics and complementation support auxin influx (PMID:29650967); they neither demonstrate nor exclude amino-acid substrates. No assay of amino-acid transport or target-specific loss was recovered. Retain explicit uncertainty rather than calling auxin specificity proof of an erroneous IBA. Propagation Review Root cause: UNRESOLVED Sources checked: PANTHER:PTN001118955 UNRESOLVED Amino-acid IBD inspected in PTHR48017 PAINT table. Target substrate range and relationship to the separately recorded IRD node remain unresolved. Supporting Evidence: PMID:29650967 This genetic assay revealed that only one of the OsAUX1 sequences (Os01g63770) was able to successfully rescue the aux1 mutant’s root agravitropic defect (Supplementary Figure 1b, c). |
| GO:0016020 membrane | IBA GO_REF:0000033 | MODIFY | Summary: Membrane can be refined to the experimentally supported plasma membrane. Reason: The broad PAINT location remains biologically correct. Native OsAUX1 localization permits refinement to GO:0005886, already represented in this review (PMID:26140668). No organelle-to-subcompartment substitution is involved. Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN001118955 SUPPORTS TRANSFER The inspected PAINT node supports membrane localization; native rice PM evidence refines that valid broad claim without alleging propagation failure. Proposed replacements: plasma membrane Supporting Evidence: PMID:26140668 However, OsAUX1, like AtAUX1, localizes at the plasma membrane and appears to function as an auxin tranporter. |
| GO:0010328 auxin influx transmembrane transporter activity | ISS PMID:29650967 Rice auxin influx carrier OsAUX1 facilitates root hair elong... | NEW | Summary: Propose the independently supported auxin influx transporter activity. Reason: This MF is absent from the four seeded rows and is not an ancestor or descendant of the amino-acid substrate claims. The exact rice locus Os01g63770 complements Arabidopsis aux1, while independent native T-DNA alleles and NAA-bypass phenotypes support auxin influx (PMID:29650967; PMID:25311360). ISS records the conserved carrier-function inference, corroborated by complementation; it does not assert a direct purified or oocyte OsAUX1 flux assay. The separate amino-acid IBAs remain unresolved. No redundant new BP is proposed. Supporting Evidence: PMID:29650967 This genetic assay revealed that only one of the OsAUX1 sequences (Os01g63770) was able to successfully rescue the aux1 mutant’s root agravitropic defect (Supplementary Figure 1b, c). PMID:26140668 However, OsAUX1, like AtAUX1, localizes at the plasma membrane and appears to function as an auxin tranporter. |
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Download this section (compressed HTML)Q: Does OsAUX1 transport any amino-acid substrates in addition to auxin, and where is the loss or retention of that capacity placed within the PAINT family?
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