PYL5, also known as RCAR5, is an intracellular abscisic acid receptor in rice that acts in the nucleus and cytosol. It binds and inhibits selected clade A PP2C phosphatases, promoting downstream SAPK kinase and bZIP transcription-factor signaling. Its receptor activity regulates ABA-responsive gene expression, germination, seedling growth and adaptation to water and salt stress. The monomeric receptor can inhibit PP2C06 without added ABA in biochemical assays, whereas its signaling output in rice protoplasts depends on ABA concentration and receptor abundance.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004864 protein phosphatase inhibitor activity | IBA GO_REF:0000033 | MODIFY | Summary: The receptor inhibits PP2C serine/threonine phosphatases. Reason: Direct PP2C06/09 assays support the more specific protein serine/threonine phosphatase inhibitor activity. The RCAR5 protein is OsPYL11 in He2014; monomeric receptor shows basal ABA-independent inhibition in vitro, while protoplast rescue depends on ABA and receptor abundance. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: GRANULARITY MISMATCH Sources checked: PANTHER:PTN001581255 SUPPORTS TRANSFER The cached PAINT IBD at this node supports the broad inherited term. Target-specific experimental evidence warrants the more precise replacement; this does not dispute node placement or the underlying transfer. Proposed replacements: protein serine/threonine phosphatase inhibitor activity Supporting Evidence: PMID:24743650 OsPYL10, 11 largely exist as monomers and can inhibit OsPP2C06 phosphatase activity without ABA. PMID:26300907 OsPYL/RCAR5 that completely suppressed OsPP2C6 activity |
| GO:0005515 protein binding | IPI PMID:22071266 A rice orthologue of the ABA receptor, OsPYL/RCAR5, is a pos... | REMOVE | Summary: The reported PP2C interaction is real but generic protein binding does not describe receptor function. Reason: The cited interaction experiments are consistent with PYL5 binding selected PP2Cs. Remove the uninformative generic binding term under the protein-binding policy; this does not reject the interaction. The mechanistic receptor and phosphatase-inhibitor functions are represented separately. Supporting Evidence: file:ORYSJ/PYL5/PYL5-uniprot.txt Intracellular abscisic acid (ABA) receptor that functions as a positive regulator of ABA signaling pathway |
| GO:0005515 protein binding | IPI PMID:26362328 Characterization and Functional Analysis of Pyrabactin Resis... | REMOVE | Summary: The reported PP2C interaction is real but generic protein binding does not describe receptor function. Reason: The cited interaction experiments are consistent with PYL5 binding selected PP2Cs. Remove the uninformative generic binding term under the protein-binding policy; this does not reject the interaction. The mechanistic receptor and phosphatase-inhibitor functions are represented separately. Supporting Evidence: file:ORYSJ/PYL5/PYL5-uniprot.txt Intracellular abscisic acid (ABA) receptor that functions as a positive regulator of ABA signaling pathway |
| GO:0005515 protein binding | IPI PMID:26362328 Characterization and Functional Analysis of Pyrabactin Resis... | REMOVE | Summary: The reported PP2C interaction is real but generic protein binding does not describe receptor function. Reason: The cited interaction experiments are consistent with PYL5 binding selected PP2Cs. Remove the uninformative generic binding term under the protein-binding policy; this does not reject the interaction. The mechanistic receptor and phosphatase-inhibitor functions are represented separately. Supporting Evidence: file:ORYSJ/PYL5/PYL5-uniprot.txt Intracellular abscisic acid (ABA) receptor that functions as a positive regulator of ABA signaling pathway |
| GO:0005515 protein binding | IPI PMID:28000033 The protein phosphatase 2C clade A protein OsPP2C51 positive... | REMOVE | Summary: The reported PP2C interaction is real but generic protein binding does not describe receptor function. Reason: The cited interaction experiments are consistent with PYL5 binding selected PP2Cs. Remove the uninformative generic binding term under the protein-binding policy; this does not reject the interaction. The mechanistic receptor and phosphatase-inhibitor functions are represented separately. Supporting Evidence: file:ORYSJ/PYL5/PYL5-uniprot.txt Intracellular abscisic acid (ABA) receptor that functions as a positive regulator of ABA signaling pathway |
| GO:0005515 protein binding | IPI PMID:28827170 Modulation of ABA Signaling by Altering VxGΦL Motif of PP2Cs... | REMOVE | Summary: The reported PP2C interaction is real but generic protein binding does not describe receptor function. Reason: The cited interaction experiments are consistent with PYL5 binding selected PP2Cs. Remove the uninformative generic binding term under the protein-binding policy; this does not reject the interaction. The mechanistic receptor and phosphatase-inhibitor functions are represented separately. Supporting Evidence: file:ORYSJ/PYL5/PYL5-uniprot.txt Intracellular abscisic acid (ABA) receptor that functions as a positive regulator of ABA signaling pathway |
| GO:0005634 nucleus | EXP PMID:28000033 The protein phosphatase 2C clade A protein OsPP2C51 positive... | ACCEPT | Summary: PYL5 is observed in both nuclear and cytosolic compartments. Reason: Reporter localization in the rice receptor-family study supports this compartment. Curated localization associated with the original reference is consistent with that independent evidence; paper-specific PYL naming is resolved by the accession and locus. Supporting Evidence: PMID:26362328 All tested OsPYLs were localized in both the cytosol and nucleus |
| GO:0005634 nucleus | IBA GO_REF:0000033 | ACCEPT | Summary: PYL5 is observed in both nuclear and cytosolic compartments. Reason: Reporter localization in the rice receptor-family study supports this compartment. Curated localization associated with the original reference is consistent with that independent evidence; paper-specific PYL naming is resolved by the accession and locus. Supporting Evidence: PMID:26362328 All tested OsPYLs were localized in both the cytosol and nucleus |
| GO:0005634 nucleus | IDA PMID:22071266 A rice orthologue of the ABA receptor, OsPYL/RCAR5, is a pos... | ACCEPT | Summary: PYL5 is observed in both nuclear and cytosolic compartments. Reason: Reporter localization in the rice receptor-family study supports this compartment. Curated localization associated with the original reference is consistent with that independent evidence; paper-specific PYL naming is resolved by the accession and locus. Supporting Evidence: PMID:26362328 All tested OsPYLs were localized in both the cytosol and nucleus |
| GO:0005634 nucleus | IDA PMID:26362328 Characterization and Functional Analysis of Pyrabactin Resis... | ACCEPT | Summary: PYL5 is observed in both nuclear and cytosolic compartments. Reason: Reporter localization in the rice receptor-family study supports this compartment. Curated localization associated with the original reference is consistent with that independent evidence; paper-specific PYL naming is resolved by the accession and locus. Supporting Evidence: PMID:26362328 All tested OsPYLs were localized in both the cytosol and nucleus |
| GO:0005634 nucleus | IEA GO_REF:0000044 | ACCEPT | Summary: PYL5 is observed in both nuclear and cytosolic compartments. Reason: Reporter localization in the rice receptor-family study supports this compartment. Curated localization associated with the original reference is consistent with that independent evidence; paper-specific PYL naming is resolved by the accession and locus. Supporting Evidence: PMID:26362328 All tested OsPYLs were localized in both the cytosol and nucleus |
| GO:0005737 cytoplasm | IBA GO_REF:0000033 | MODIFY | Summary: Cytoplasm can be refined to the observed cytosolic location. Reason: The direct localization experiments resolve the cytoplasmic signal to cytosol, which is already separately annotated. Nuclear localization is retained independently. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: GRANULARITY MISMATCH Sources checked: PANTHER:PTN001581255 SUPPORTS TRANSFER The cached PAINT IBD at this node supports the broad inherited term. Target-specific experimental evidence warrants the more precise replacement; this does not dispute node placement or the underlying transfer. Proposed replacements: cytosol Supporting Evidence: PMID:26362328 All tested OsPYLs were localized in both the cytosol and nucleus |
| GO:0005829 cytosol | IDA PMID:26362328 Characterization and Functional Analysis of Pyrabactin Resis... | ACCEPT | Summary: PYL5 is observed in both nuclear and cytosolic compartments. Reason: Reporter localization in the rice receptor-family study supports this compartment. Curated localization associated with the original reference is consistent with that independent evidence; paper-specific PYL naming is resolved by the accession and locus. Supporting Evidence: PMID:26362328 All tested OsPYLs were localized in both the cytosol and nucleus |
| GO:0005829 cytosol | IEA GO_REF:0000120 | ACCEPT | Summary: PYL5 is observed in both nuclear and cytosolic compartments. Reason: Reporter localization in the rice receptor-family study supports this compartment. Curated localization associated with the original reference is consistent with that independent evidence; paper-specific PYL naming is resolved by the accession and locus. Supporting Evidence: PMID:26362328 All tested OsPYLs were localized in both the cytosol and nucleus |
| GO:0006970 response to osmotic stress | IMP PMID:24474809 Overexpression of PYL5 in rice enhances drought tolerance, i... | KEEP AS NON CORE | Summary: PYL5 influences physiological responses to abiotic stress through ABA signaling. Reason: PYL5 overexpression alters stress-responsive transcription and drought, salt and osmotic phenotypes (PMID:24474809). These are supported organism-level consequences of its core ABA receptor role, not additional catalytic activities. Supporting Evidence: PMID:24474809 resulted in improved drought and salt stress tolerance in rice. |
| GO:0009414 response to water deprivation | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: PYL5 influences physiological responses to abiotic stress through ABA signaling. Reason: PYL5 overexpression alters stress-responsive transcription and drought, salt and osmotic phenotypes (PMID:24474809). These are supported organism-level consequences of its core ABA receptor role, not additional catalytic activities. Supporting Evidence: PMID:24474809 resulted in improved drought and salt stress tolerance in rice. |
| GO:0009414 response to water deprivation | IMP PMID:24474809 Overexpression of PYL5 in rice enhances drought tolerance, i... | KEEP AS NON CORE | Summary: PYL5 influences physiological responses to abiotic stress through ABA signaling. Reason: PYL5 overexpression alters stress-responsive transcription and drought, salt and osmotic phenotypes (PMID:24474809). These are supported organism-level consequences of its core ABA receptor role, not additional catalytic activities. Supporting Evidence: PMID:24474809 resulted in improved drought and salt stress tolerance in rice. |
| GO:0009651 response to salt stress | IMP PMID:24474809 Overexpression of PYL5 in rice enhances drought tolerance, i... | KEEP AS NON CORE | Summary: PYL5 influences physiological responses to abiotic stress through ABA signaling. Reason: PYL5 overexpression alters stress-responsive transcription and drought, salt and osmotic phenotypes (PMID:24474809). These are supported organism-level consequences of its core ABA receptor role, not additional catalytic activities. Supporting Evidence: PMID:24474809 resulted in improved drought and salt stress tolerance in rice. |
| GO:0009738 abscisic acid-activated signaling pathway | IBA GO_REF:0000033 | ACCEPT | Summary: PYL5 is a positive receptor component of ABA signaling. Reason: Biochemical inhibition and rice-protoplast reconstitution independently establish PYL5 upstream of PP2C, SAPK and bZIP components. Retain the same biological conclusion across experimental and inferred rows; the initial RCAR5βPP2C30βSAPK2βOREB1 relay and the later PP2C6βSAPK9βBZIP46 relay are distinct tested combinations. Supporting Evidence: file:ORYSJ/PYL5/PYL5-uniprot.txt Intracellular abscisic acid (ABA) receptor that functions as a positive regulator of ABA signaling pathway PMID:24743650 OsPYL10, 11 largely exist as monomers and can inhibit OsPP2C06 phosphatase activity without ABA. PMID:26300907 OsPYL/RCAR5 that completely suppressed OsPP2C6 activity |
| GO:0009738 abscisic acid-activated signaling pathway | IDA PMID:22071266 A rice orthologue of the ABA receptor, OsPYL/RCAR5, is a pos... | ACCEPT | Summary: PYL5 is a positive receptor component of ABA signaling. Reason: Biochemical inhibition and rice-protoplast reconstitution independently establish PYL5 upstream of PP2C, SAPK and bZIP components. Retain the same biological conclusion across experimental and inferred rows; the initial RCAR5βPP2C30βSAPK2βOREB1 relay and the later PP2C6βSAPK9βBZIP46 relay are distinct tested combinations. Supporting Evidence: file:ORYSJ/PYL5/PYL5-uniprot.txt Intracellular abscisic acid (ABA) receptor that functions as a positive regulator of ABA signaling pathway PMID:24743650 OsPYL10, 11 largely exist as monomers and can inhibit OsPP2C06 phosphatase activity without ABA. PMID:26300907 OsPYL/RCAR5 that completely suppressed OsPP2C6 activity |
| GO:0009738 abscisic acid-activated signaling pathway | IDA PMID:24743650 Identification and characterization of ABA receptors in Oryz... | ACCEPT | Summary: PYL5 is a positive receptor component of ABA signaling. Reason: Biochemical inhibition and rice-protoplast reconstitution independently establish PYL5 upstream of PP2C, SAPK and bZIP components. Retain the same biological conclusion across experimental and inferred rows; the initial RCAR5βPP2C30βSAPK2βOREB1 relay and the later PP2C6βSAPK9βBZIP46 relay are distinct tested combinations. Supporting Evidence: file:ORYSJ/PYL5/PYL5-uniprot.txt Intracellular abscisic acid (ABA) receptor that functions as a positive regulator of ABA signaling pathway PMID:24743650 OsPYL10, 11 largely exist as monomers and can inhibit OsPP2C06 phosphatase activity without ABA. PMID:26300907 OsPYL/RCAR5 that completely suppressed OsPP2C6 activity |
| GO:0009738 abscisic acid-activated signaling pathway | IDA PMID:28000033 The protein phosphatase 2C clade A protein OsPP2C51 positive... | ACCEPT | Summary: PYL5 is a positive receptor component of ABA signaling. Reason: Biochemical inhibition and rice-protoplast reconstitution independently establish PYL5 upstream of PP2C, SAPK and bZIP components. Retain the same biological conclusion across experimental and inferred rows; the initial RCAR5βPP2C30βSAPK2βOREB1 relay and the later PP2C6βSAPK9βBZIP46 relay are distinct tested combinations. Supporting Evidence: file:ORYSJ/PYL5/PYL5-uniprot.txt Intracellular abscisic acid (ABA) receptor that functions as a positive regulator of ABA signaling pathway PMID:24743650 OsPYL10, 11 largely exist as monomers and can inhibit OsPP2C06 phosphatase activity without ABA. PMID:26300907 OsPYL/RCAR5 that completely suppressed OsPP2C6 activity |
| GO:0009738 abscisic acid-activated signaling pathway | IEA GO_REF:0000117 | ACCEPT | Summary: PYL5 is a positive receptor component of ABA signaling. Reason: Biochemical inhibition and rice-protoplast reconstitution independently establish PYL5 upstream of PP2C, SAPK and bZIP components. Retain the same biological conclusion across experimental and inferred rows; the initial RCAR5βPP2C30βSAPK2βOREB1 relay and the later PP2C6βSAPK9βBZIP46 relay are distinct tested combinations. Supporting Evidence: file:ORYSJ/PYL5/PYL5-uniprot.txt Intracellular abscisic acid (ABA) receptor that functions as a positive regulator of ABA signaling pathway PMID:24743650 OsPYL10, 11 largely exist as monomers and can inhibit OsPP2C06 phosphatase activity without ABA. PMID:26300907 OsPYL/RCAR5 that completely suppressed OsPP2C6 activity |
| GO:0009738 abscisic acid-activated signaling pathway | IMP PMID:24474809 Overexpression of PYL5 in rice enhances drought tolerance, i... | ACCEPT | Summary: PYL5 is a positive receptor component of ABA signaling. Reason: Biochemical inhibition and rice-protoplast reconstitution independently establish PYL5 upstream of PP2C, SAPK and bZIP components. Retain the same biological conclusion across experimental and inferred rows; the initial RCAR5βPP2C30βSAPK2βOREB1 relay and the later PP2C6βSAPK9βBZIP46 relay are distinct tested combinations. Supporting Evidence: file:ORYSJ/PYL5/PYL5-uniprot.txt Intracellular abscisic acid (ABA) receptor that functions as a positive regulator of ABA signaling pathway PMID:24743650 OsPYL10, 11 largely exist as monomers and can inhibit OsPP2C06 phosphatase activity without ABA. PMID:26300907 OsPYL/RCAR5 that completely suppressed OsPP2C6 activity |
| GO:0009845 seed germination | IEA GO_REF:0000117 | KEEP AS NON CORE | Summary: ABA-responsive germination and early seedling growth depend on receptor signaling. Reason: RCAR5 overexpression alters ABA sensitivity during these developmental stages (PMID:22071266). The terms are retained as developmental effects of the receptor; they do not imply that PYL5 promotes germination independently of ABA. Supporting Evidence: PMID:22071266 hypersensitive to ABA during seed germination and early seedling growth. |
| GO:0009845 seed germination | IMP PMID:22071266 A rice orthologue of the ABA receptor, OsPYL/RCAR5, is a pos... | KEEP AS NON CORE | Summary: ABA-responsive germination and early seedling growth depend on receptor signaling. Reason: RCAR5 overexpression alters ABA sensitivity during these developmental stages (PMID:22071266). The terms are retained as developmental effects of the receptor; they do not imply that PYL5 promotes germination independently of ABA. Supporting Evidence: PMID:22071266 hypersensitive to ABA during seed germination and early seedling growth. |
| GO:0010427 abscisic acid binding | IBA GO_REF:0000033 | ACCEPT | Summary: ABA recognition is intrinsic to the intracellular receptor. Reason: The curated receptor function and biochemical study of rice PYLs support ABA binding. Basal PP2C inhibition by a monomeric receptor does not imply failure to bind or respond to ABA. Supporting Evidence: file:ORYSJ/PYL5/PYL5-uniprot.txt Intracellular abscisic acid (ABA) receptor that functions as a positive regulator of ABA signaling pathway |
| GO:0038023 signaling receptor activity | IBA GO_REF:0000033 | ACCEPT | Summary: Intracellular signaling receptor activity correctly describes PYL5. Reason: PYL5 couples ABA recognition to PP2C inhibition and downstream signaling. The receptor term captures this signal-transducing role; it is not merely ligand binding. Supporting Evidence: file:ORYSJ/PYL5/PYL5-uniprot.txt Intracellular abscisic acid (ABA) receptor that functions as a positive regulator of ABA signaling pathway |
| GO:0090351 seedling development | IMP PMID:22071266 A rice orthologue of the ABA receptor, OsPYL/RCAR5, is a pos... | KEEP AS NON CORE | Summary: ABA-responsive germination and early seedling growth depend on receptor signaling. Reason: RCAR5 overexpression alters ABA sensitivity during these developmental stages (PMID:22071266). The terms are retained as developmental effects of the receptor; they do not imply that PYL5 promotes germination independently of ABA. Supporting Evidence: PMID:22071266 hypersensitive to ABA during seed germination and early seedling growth. |
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