CASP-like protein 1F2 (PtCASPL1F2) is a small tetraspan membrane protein belonging to the CASP-like (CASPL) subfamily, the more divergent relatives of the bona fide Casparian strip membrane domain proteins (CASP1-5). Like other family members it has a central MARVEL-like four-transmembrane domain and is predicted to reside in the plasma membrane as a multi-pass protein. CASP-like proteins are not enzymes; by analogy with the characterized CASP scaffolds they are thought to organize plasma-membrane microdomains, but the specific biological role and tissue context of CASPL1F2 remain uncharacterized. The protein is predicted to form homodimers and heterodimers. In poplar, PtCASPL1F2 is expressed predominantly in developing xylem (xylem vessels, ~34 TPM) and is broadly abiotic-stress-responsive (EBI Expression Atlas E-GEOD-81077 / E-MTAB-5540).
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005886 plasma membrane | IEA GO_REF:0000120 | ACCEPT | Summary: CASPL1F2 is a multi-pass membrane protein of the CASP-like family; plasma membrane localization is the supported cellular component and the only well-grounded annotation for this otherwise uncharacterized protein. Reason: UniProt reports cell membrane (plasma membrane) localization as a multi-pass membrane protein, consistent with the CASP/CASPL family and the four predicted transmembrane helices in the sequence record. Supporting Evidence: file:POPTR/CASPL1F2/CASPL1F2-uniprot.txt SUBCELLULAR LOCATION: Cell membrane file:POPTR/CASPL1F2/CASPL1F2-uniprot.txt DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. |
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Download this section (compressed HTML)Q: In which tissues is PtCASPL1F2 expressed, and does it organize a distinct plasma-membrane microdomain like the bona fide CASP proteins or serve a different role?
Experiment: Determine the expression pattern and subcellular localization of tagged PtCASPL1F2 across poplar tissues to identify where it acts and whether it forms membrane microdomains.
Type: expression and localization profiling
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