Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0006412 translation	IBA GO_REF:0000033	ACCEPT	Summary: Translation is the core biological process for this plastid ribosomal protein. Reason: The protein is a chloroplast ribosomal subunit component supporting plastid translation. Supporting Evidence: file:POPTR/rps12-A/rps12-A-uniprot.txt RecName: Full=Small ribosomal subunit protein uS12cz
GO:0005840 ribosome	IBA GO_REF:0000033	KEEP AS NON CORE	Summary: Generic ribosome is correct but less specific than the plastid ribosomal subunit term. Reason: Retain the generic ribosome annotation as non-core because the small/large ribosomal subunit term is more precise for this protein. Supporting Evidence: file:POPTR/rps12-A/rps12-A-uniprot.txt RecName: Full=Small ribosomal subunit protein uS12cz
GO:0003735 structural constituent of ribosome	IEA GO_REF:0000002	ACCEPT	Summary: Structural constituent of ribosome is supported for this chloroplast ribosomal protein. Reason: The reviewed entry identifies the protein as a conserved chloroplast ribosomal subunit protein. Supporting Evidence: file:POPTR/rps12-A/rps12-A-uniprot.txt RecName: Full=Small ribosomal subunit protein uS12cz
GO:0005840 ribosome	IEA GO_REF:0000002	KEEP AS NON CORE	Summary: Generic ribosome is correct but less specific than the plastid ribosomal subunit term. Reason: Retain the generic ribosome annotation as non-core because the small/large ribosomal subunit term is more precise for this protein. Supporting Evidence: file:POPTR/rps12-A/rps12-A-uniprot.txt RecName: Full=Small ribosomal subunit protein uS12cz
GO:0006412 translation	IEA GO_REF:0000120	ACCEPT	Summary: Translation is the core biological process for this plastid ribosomal protein. Reason: The protein is a chloroplast ribosomal subunit component supporting plastid translation. Supporting Evidence: file:POPTR/rps12-A/rps12-A-uniprot.txt RecName: Full=Small ribosomal subunit protein uS12cz
GO:0009507 chloroplast	IEA GO_REF:0000120	ACCEPT	Summary: Chloroplast localization is correct for rps12-A. Reason: UniProt places this ribosomal protein in the chloroplast. Supporting Evidence: file:POPTR/rps12-A/rps12-A-uniprot.txt SUBCELLULAR LOCATION: Plastid, chloroplast.
GO:0015935 small ribosomal subunit	IEA GO_REF:0000002	ACCEPT	Summary: small ribosomal subunit is the appropriate ribosomal complex term for rps12-A. Reason: The reviewed entry identifies this as a chloroplast small/large ribosomal subunit protein. Supporting Evidence: file:POPTR/rps12-A/rps12-A-uniprot.txt RecName: Full=Small ribosomal subunit protein uS12cz
GO:0019843 rRNA binding	IEA GO_REF:0000104	MODIFY	Summary: Generic rRNA binding is correct but less specific than small ribosomal subunit rRNA binding. Reason: For this chloroplast small-subunit ribosomal protein, the small-ribosomal-subunit rRNA binding term is more precise than generic rRNA binding. Proposed replacements: small ribosomal subunit rRNA binding Supporting Evidence: file:POPTR/rps12-A/rps12-A-uniprot.txt DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.

Core Functions

Acts as a chloroplast small-ribosomal-subunit protein supporting plastid translation.

Molecular Function:

structural constituent of ribosome

Directly Involved In:

translation

Cellular Locations:

chloroplast

In Complex:

small ribosomal subunit

Supporting Evidence:

file:POPTR/rps12-A/rps12-A-uniprot.txt
RecName: Full=Small ribosomal subunit protein uS12cz
file:POPTR/rps12-A/rps12-A-uniprot.txt
With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits
file:POPTR/rps12-A/rps12-A-uniprot.txt
SUBCELLULAR LOCATION: Plastid, chloroplast.

Binds rRNA as part of the chloroplast small ribosomal subunit.

Molecular Function:

small ribosomal subunit rRNA binding

Directly Involved In:

translation

In Complex:

small ribosomal subunit

Supporting Evidence:

file:POPTR/rps12-A/rps12-A-uniprot.txt
With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits

References

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Suggested Experiments

Experiment: Measure plastid ribosome assembly and chloroplast translation products in rps12-A perturbation or plastid-editing lines.

Type: targeted functional assay

📚 Additional Documentation

Notes

(rps12-A-notes.md)

rps12-A notes

UniProt identifies rps12-A as Small ribosomal subunit protein uS12cz [file:POPTR/rps12-A/rps12-A-uniprot.txt "RecName: Full=Small ribosomal subunit protein uS12cz"].
UniProt places rps12-A in the chloroplast [file:POPTR/rps12-A/rps12-A-uniprot.txt "SUBCELLULAR LOCATION: Plastid, chloroplast"].
Reviewed the ribosomal structural and translation annotations as core, while correcting compartment over-annotations to the plastid small ribosomal subunit context where needed.

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