rps12-B

UniProt ID: A4GYN8
Organism: Populus trichocarpa
Review Status: DRAFT
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Gene Description

Chloroplast small ribosomal subunit protein uS12cy that contributes to plastid ribosome structure, rRNA binding, and translation.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0006412 translation
IBA
GO_REF:0000033 		ACCEPT
Summary: Translation is the correct biological process for a chloroplast ribosomal protein.
Reason: The protein functions in the chloroplast ribosome and supports plastid translation.
Supporting Evidence:
file:POPTR/rps12-B/rps12-B-uniprot.txt
With S4 and S5 plays an important role in translational accuracy.
GO:0005840 ribosome
IBA
GO_REF:0000033 		KEEP AS NON CORE
Summary: Ribosome is correct but less specific than chloroplast small ribosomal subunit.
Reason: Retain the generic ribosome annotation as non-core because the small-ribosomal-subunit term is more precise.
Supporting Evidence:
file:POPTR/rps12-B/rps12-B-uniprot.txt
Part of the 30S ribosomal subunit.
GO:0003735 structural constituent of ribosome
IEA
GO_REF:0000002 		ACCEPT
Summary: Structural constituent of ribosome is the core molecular function for a chloroplast ribosomal protein.
Reason: rps12-B is a conserved chloroplast small-subunit ribosomal protein.
Supporting Evidence:
file:POPTR/rps12-B/rps12-B-uniprot.txt
RecName: Full=Small ribosomal subunit protein uS12cy
GO:0005840 ribosome
IEA
GO_REF:0000002 		KEEP AS NON CORE
Summary: Ribosome is correct but less specific than chloroplast small ribosomal subunit.
Reason: Retain the generic ribosome annotation as non-core because the small-ribosomal-subunit term is more precise.
Supporting Evidence:
file:POPTR/rps12-B/rps12-B-uniprot.txt
Part of the 30S ribosomal subunit.
GO:0006412 translation
IEA
GO_REF:0000120 		ACCEPT
Summary: Translation is the correct biological process for a chloroplast ribosomal protein.
Reason: The protein functions in the chloroplast ribosome and supports plastid translation.
Supporting Evidence:
file:POPTR/rps12-B/rps12-B-uniprot.txt
With S4 and S5 plays an important role in translational accuracy.
GO:0009507 chloroplast
IEA
GO_REF:0000120 		ACCEPT
Summary: Chloroplast localization is correct for this plastid-encoded ribosomal protein.
Reason: The UniProt entry places the protein in the chloroplast.
Supporting Evidence:
file:POPTR/rps12-B/rps12-B-uniprot.txt
SUBCELLULAR LOCATION: Plastid, chloroplast
GO:0015935 small ribosomal subunit
IEA
GO_REF:0000002 		ACCEPT
Summary: rps12-B is part of the chloroplast small ribosomal subunit.
Reason: The term correctly captures its ribosomal-complex context.
Supporting Evidence:
file:POPTR/rps12-B/rps12-B-uniprot.txt
RecName: Full=Small ribosomal subunit protein uS12cy
GO:0019843 rRNA binding
IEA
GO_REF:0000104 		ACCEPT
Summary: rRNA binding is accepted as a UniProtKB-UniRule family annotation for chloroplast uS12/rps12 proteins.
Reason: The rps12-B entry lacks a protein-specific rRNA-contact statement, so this ACCEPT is explicitly rule-based rather than experimental for Populus.
Supporting Evidence:
file:POPTR/rps12-B/rps12-B-uniprot.txt
DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.

Core Functions

Acts as a chloroplast small-ribosomal-subunit protein that contributes to ribosome structure, rRNA binding, and translation.

Molecular Function:
structural constituent of ribosome
Directly Involved In:
translation
Cellular Locations:
chloroplast
In Complex:
small ribosomal subunit
Supporting Evidence:
  • file:POPTR/rps12-B/rps12-B-uniprot.txt
    RecName: Full=Small ribosomal subunit protein uS12cy
  • file:POPTR/rps12-B/rps12-B-uniprot.txt
    Part of the 30S ribosomal subunit.
  • file:POPTR/rps12-B/rps12-B-uniprot.txt
    SUBCELLULAR LOCATION: Plastid, chloroplast

Has rule-based rRNA binding annotation as part of the chloroplast small ribosomal subunit.

Molecular Function:
rRNA binding
Directly Involved In:
translation
In Complex:
small ribosomal subunit
Supporting Evidence:
  • file:POPTR/rps12-B/rps12-B-uniprot.txt
    DR GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.

References

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Suggested Questions for Experts

Q: Are any Populus-specific plastid translation phenotypes associated with rps12-B perturbation beyond the conserved ribosomal role?

Suggested Experiments

Experiment: Test chloroplast translation and small-ribosomal-subunit assembly in rps12-B perturbation lines.

Type: targeted functional assay

📚 Additional Documentation

Notes

(rps12-B-notes.md)

rps12-B notes

  • Fetched with just fetch-gene POPTR rps12-B.

  • Curated as chloroplast small ribosomal subunit protein uS12cy. Generic ribosome terms are retained as non-core where the small ribosomal subunit term is more specific; structural ribosome function, rRNA binding, chloroplast localization, and translation are core.

  • Addressed PR #469 review on 2026-05-10: replaced circular rRNA-binding support from the UniProt GO cross-reference with the independent UniProt subunit statement Part of the 30S ribosomal subunit.

  • Addressed PR #469 re-review on 2026-05-10: rRNA binding support now uses the UniProtKB-UniRule GO DR line and states explicitly that the ACCEPT is rule-based for the uS12 family, not a direct Populus rRNA-contact assay.

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