id: A4GYN8
gene_symbol: rps12-B
product_type: PROTEIN
status: DRAFT
taxon:
  id: NCBITaxon:3694
  label: Populus trichocarpa
description: Chloroplast small ribosomal subunit protein uS12cy that contributes to plastid ribosome
  structure, rRNA binding, and translation.
existing_annotations:
- term:
    id: GO:0006412
    label: translation
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review: &id002
    summary: Translation is the correct biological process for a chloroplast ribosomal protein.
    action: ACCEPT
    reason: The protein functions in the chloroplast ribosome and supports plastid translation.
    supported_by:
    - reference_id: file:POPTR/rps12-B/rps12-B-uniprot.txt
      supporting_text: With S4 and S5 plays an important role in translational accuracy.
- term:
    id: GO:0005840
    label: ribosome
  evidence_type: IBA
  original_reference_id: GO_REF:0000033
  review: &id001
    summary: Ribosome is correct but less specific than chloroplast small ribosomal subunit.
    action: KEEP_AS_NON_CORE
    reason: Retain the generic ribosome annotation as non-core because the small-ribosomal-subunit 
      term is more precise.
    supported_by:
    - reference_id: file:POPTR/rps12-B/rps12-B-uniprot.txt
      supporting_text: Part of the 30S ribosomal subunit.
- term:
    id: GO:0003735
    label: structural constituent of ribosome
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  review:
    summary: Structural constituent of ribosome is the core molecular function for a chloroplast 
      ribosomal protein.
    action: ACCEPT
    reason: rps12-B is a conserved chloroplast small-subunit ribosomal protein.
    supported_by:
    - reference_id: file:POPTR/rps12-B/rps12-B-uniprot.txt
      supporting_text: 'RecName: Full=Small ribosomal subunit protein uS12cy'
- term:
    id: GO:0005840
    label: ribosome
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  review: *id001
- term:
    id: GO:0006412
    label: translation
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review: *id002
- term:
    id: GO:0009507
    label: chloroplast
  evidence_type: IEA
  original_reference_id: GO_REF:0000120
  review:
    summary: Chloroplast localization is correct for this plastid-encoded ribosomal protein.
    action: ACCEPT
    reason: The UniProt entry places the protein in the chloroplast.
    supported_by:
    - reference_id: file:POPTR/rps12-B/rps12-B-uniprot.txt
      supporting_text: 'SUBCELLULAR LOCATION: Plastid, chloroplast'
- term:
    id: GO:0015935
    label: small ribosomal subunit
  evidence_type: IEA
  original_reference_id: GO_REF:0000002
  review:
    summary: rps12-B is part of the chloroplast small ribosomal subunit.
    action: ACCEPT
    reason: The term correctly captures its ribosomal-complex context.
    supported_by:
    - reference_id: file:POPTR/rps12-B/rps12-B-uniprot.txt
      supporting_text: 'RecName: Full=Small ribosomal subunit protein uS12cy'
  qualifier: part_of
- term:
    id: GO:0019843
    label: rRNA binding
  evidence_type: IEA
  original_reference_id: GO_REF:0000104
  review:
    summary: rRNA binding is accepted as a UniProtKB-UniRule family annotation for chloroplast 
      uS12/rps12 proteins.
    action: ACCEPT
    reason: The rps12-B entry lacks a protein-specific rRNA-contact statement, so this ACCEPT is 
      explicitly rule-based rather than experimental for Populus.
    supported_by:
    - reference_id: file:POPTR/rps12-B/rps12-B-uniprot.txt
      supporting_text: DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
references:
- id: GO_REF:0000002
  title: Gene Ontology annotation through association of InterPro records with GO terms
  findings:
  - statement: GO_REF:0000002 supplied one or more source GOA annotations reviewed in this file.
- id: GO_REF:0000033
  title: Annotation inferences using phylogenetic trees
  findings:
  - statement: GO_REF:0000033 supplied one or more source GOA annotations reviewed in this file.
- id: GO_REF:0000104
  title: Electronic Gene Ontology annotations created by transferring manual GO annotations between 
    related proteins based on shared sequence features
  findings:
  - statement: GO_REF:0000104 supplied one or more source GOA annotations reviewed in this file.
- id: GO_REF:0000120
  title: Combined Automated Annotation using Multiple IEA Methods
  findings:
  - statement: GO_REF:0000120 supplied one or more source GOA annotations reviewed in this file.
- id: file:POPTR/rps12-B/rps12-B-uniprot.txt
  title: UniProtKB reviewed entry for rps12-B
  findings:
  - statement: UniProt identifies rps12-B as chloroplast small ribosomal subunit protein uS12cy, 
      part of the 30S/small ribosomal subunit involved in plastid translation.
- id: file:POPTR/rps12-B/rps12-B-goa.tsv
  title: QuickGO GOA annotations for rps12-B
  findings:
  - statement: GOA rows supplied the existing_annotations reviewed in this file.
core_functions:
- description: Acts as a chloroplast small-ribosomal-subunit protein that contributes to ribosome 
    structure, rRNA binding, and translation.
  molecular_function:
    id: GO:0003735
    label: structural constituent of ribosome
  directly_involved_in:
  - &id003
    id: GO:0006412
    label: translation
  locations:
  - id: GO:0009507
    label: chloroplast
  in_complex: &id004
    id: GO:0015935
    label: small ribosomal subunit
  supported_by:
  - reference_id: file:POPTR/rps12-B/rps12-B-uniprot.txt
    supporting_text: 'RecName: Full=Small ribosomal subunit protein uS12cy'
  - reference_id: file:POPTR/rps12-B/rps12-B-uniprot.txt
    supporting_text: Part of the 30S ribosomal subunit.
  - reference_id: file:POPTR/rps12-B/rps12-B-uniprot.txt
    supporting_text: 'SUBCELLULAR LOCATION: Plastid, chloroplast'
- description: Has rule-based rRNA binding annotation as part of the chloroplast small ribosomal 
    subunit.
  molecular_function:
    id: GO:0019843
    label: rRNA binding
  directly_involved_in:
  - *id003
  locations: []
  in_complex: *id004
  supported_by:
  - reference_id: file:POPTR/rps12-B/rps12-B-uniprot.txt
    supporting_text: DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
proposed_new_terms: []
suggested_questions:
- question: Are any Populus-specific plastid translation phenotypes associated with rps12-B 
    perturbation beyond the conserved ribosomal role?
suggested_experiments:
- description: Test chloroplast translation and small-ribosomal-subunit assembly in rps12-B 
    perturbation lines.
  experiment_type: targeted functional assay