id: A0AB35LIB0
gene_symbol: fosA3
product_type: PROTEIN
status: DRAFT
taxon:
  id: NCBITaxon:587
  label: Providencia rettgeri
description: fosA3 encodes a FosA-family fosfomycin resistance enzyme in Providencia rettgeri. UniProt/CARD identify this entry as a glutathione-dependent fosfomycin-modifying enzyme; the defensible GO molecular function is glutathione transferase activity for FosA-family members. UniProt names the protein 'Fosfomycin resistance glutathione transferase FosA3' (accession A0AB35LIB0).
existing_annotations:
- term:
    id: GO:0004364
    label: glutathione transferase activity
  evidence_type: RCA
  original_reference_id: file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml
  qualifier: enables
  review:
    summary: 'NEW candidate annotation from the curated ARO->GO mapping: glutathione transferase activity.'
    action: NEW
    reason: The UniProt record cross-references a CARD/ARO AMR determinant, and the curated ARO->GO mapping projects the family-specific molecular function glutathione transferase activity. This focused draft accepts the candidate as a curator lead for this AMR determinant.
    additional_reference_ids:
    - file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv
    - file:genes/PRORE/fosA3/fosA3-uniprot.txt
    supported_by:
    - reference_id: file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv
      supporting_text: "A0AB35LIB0\tARO:3002872\tARO:3002872\texact\tskos:relatedMatch\tGO:0004364\tglutathione transferase activity"
    - reference_id: file:genes/PRORE/fosA3/fosA3-uniprot.txt
      supporting_text: DR   CARD; ARO:3002872; FosA3; ARO:0001004; antibiotic inactivation.
- term:
    id: GO:0046677
    label: response to antibiotic
  evidence_type: RCA
  original_reference_id: file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml
  qualifier: involved_in
  review:
    summary: 'NEW process annotation: fosA3 is an AMR determinant involved in antibiotic response/resistance.'
    action: NEW
    reason: CARD/ARO identity places this gene in an antimicrobial-resistance determinant family; response to antibiotic is the appropriate high-level GO biological process for the resistance role.
    additional_reference_ids:
    - file:genes/PRORE/fosA3/fosA3-uniprot.txt
    supported_by:
    - reference_id: file:genes/PRORE/fosA3/fosA3-uniprot.txt
      supporting_text: DR   CARD; ARO:3002872; FosA3; ARO:0001004; antibiotic inactivation.
references:
- id: file:genes/PRORE/fosA3/fosA3-uniprot.txt
  title: UniProt flat file for fosA3 (A0AB35LIB0)
  findings:
  - statement: UniProt/CARD identity and protein naming for fosA3.
    supporting_text: DR   CARD; ARO:3002872; FosA3; ARO:0001004; antibiotic inactivation.
- id: file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml
  title: Curated ARO to GO mapping set for AMR gene families
  findings:
  - statement: Manual ARO-to-GO mappings used to identify focused AMR annotation gains.
    supporting_text: 'mapping_set_title: ARO to GO mapping (AMR gene families and resistance mechanisms)'
- id: file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv
  title: Annotation-gain candidates from ARO to GO mappings
  findings:
  - statement: Candidate GO annotations projected from the ARO-to-GO mapping set.
    supporting_text: Candidate GO annotations that UniProtKB entries with a CARD cross-reference would gain from the
core_functions:
- description: fosA3 inactivates fosfomycin through glutathione-dependent modification, contributing to antibiotic resistance.
  molecular_function:
    id: GO:0004364
    label: glutathione transferase activity
  directly_involved_in:
  - id: GO:0046677
    label: response to antibiotic
  supported_by:
  - reference_id: file:genes/PRORE/fosA3/fosA3-uniprot.txt
    supporting_text: DR   CARD; ARO:3002872; FosA3; ARO:0001004; antibiotic inactivation.
suggested_questions:
- question: Is the ARO-derived glutathione transferase activity annotation sufficiently specific for fosA3, or is a narrower substrate/site-specific GO term warranted?
  experts: []
suggested_experiments:
- description: Biochemically assay purified fosA3 against representative antibiotic substrates for the inferred AMR family and measure loss of drug activity or target modification.
  experiment_type: in vitro enzyme assay