PA0011 (HtrB1) is one of two HtrB/LpxL-type lipid A late acyltransferases of Pseudomonas aeruginosa. It belongs to the lipid A biosynthesis acyltransferase family (Pfam Lip_A_acyltrans) and, with HtrB2 (PA3242), adds the secondary laurate or 2-hydroxylaurate chains of lipid A, each enzyme acting at a specific site. It is expected to sit in the inner membrane like other family members. Mutants are more susceptible to several antibiotics, cationic antimicrobial peptides and environmental stresses and have a more permeable outer membrane.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005886 plasma membrane | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt subcellular location mapping (cell inner membrane, ARBA). Reason: Lipid A late acyltransferases of this family are inner-membrane enzymes (lpxL, HAMAP MF_01942); the location fits the family. Supporting Evidence: file:PSEAE/PA0011/PA0011-uniprot.txt PF03279; Lip_A_acyltrans |
| GO:0009245 lipid A biosynthetic process | IDA PMID:26223882 Site-specific activity of the acyltransferases HtrB1 and Htr... | ACCEPT | Summary: PseudoCAP IDA row (seeds PTN004745460 glycolipid biosynthetic process). Reason: HtrB1 acylates lipid A; seed verdict SOUND. Supporting Evidence: PMID:26223882 In this study, we were able to show that each gene identified is responsible for site-specific modification of lipid A. |
| GO:0009247 glycolipid biosynthetic process | IBA GO_REF:0000033 | TOO GENERAL | Summary: IBA from PTN004745460. Reason: Entailed by GO:0009245 lipid A biosynthetic process, accepted here. |
| GO:0009247 glycolipid biosynthetic process | IEA GO_REF:0000117 | TOO GENERAL | Summary: ARBA mapping. Reason: Entailed by GO:0009245. |
| GO:0016020 membrane | IBA GO_REF:0000033 | TOO GENERAL | Summary: IBA membrane from PTN004745460. Reason: Entailed by GO:0005886 plasma membrane, accepted here. |
| GO:0016020 membrane | IEA GO_REF:0000002 | TOO GENERAL | Summary: InterPro membrane mapping. Reason: Entailed by GO:0005886. |
| GO:0016740 transferase activity | IEA GO_REF:0000002 | TOO GENERAL | Summary: InterPro mapping. Reason: Entailed by GO:0016747. |
| GO:0016746 acyltransferase activity | IBA GO_REF:0000033 | TOO GENERAL | Summary: IBA from PTN004745460, seeded by E. coli LpxL, LpxM, LpxP, M. tuberculosis PatA, lpxL and this gene. Reason: True; entailed by GO:0016747, accepted here. |
| GO:0016746 acyltransferase activity | IEA GO_REF:0000117 | TOO GENERAL | Summary: ARBA mapping. Reason: Entailed by GO:0016747. |
| GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups | IDA PMID:26223882 Site-specific activity of the acyltransferases HtrB1 and Htr... | ACCEPT | Summary: PseudoCAP IDA seed of PTN004745460 (node term acyltransferase activity). Reason: HtrB1 is responsible for site-specific acylation of lipid A; seed verdict SOUND. Kept at this level because the acceptor and donor of HtrB1 have not been read from the full text. Supporting Evidence: PMID:26223882 In this study, we were able to show that each gene identified is responsible for site-specific modification of lipid A. file:PSEAE/PA0011/PA0011-uniprot.txt PF03279; Lip_A_acyltrans |
| GO:0034605 cellular response to heat | IMP PMID:22146126 Characterization of a novel gene related to antibiotic susce... | MARK AS OVER ANNOTATED | Summary: IMP from mutant stress susceptibility. Reason: The abstract reports a mutant susceptibility phenotype; an envelope-building enzyme whose loss weakens the outer membrane is not part of a response to heat. Supporting Evidence: PMID:22146126 We found that the PA0011 mutant strain was susceptible to several different antibiotics and environmental stresses. |
| GO:0071236 cellular response to antibiotic | IMP PMID:22146126 Characterization of a novel gene related to antibiotic susce... | MARK AS OVER ANNOTATED | Summary: IMP from mutant antibiotic susceptibility. Reason: Indirect envelope-integrity phenotype, not a response to antibiotic. Supporting Evidence: PMID:22146126 We found that the PA0011 mutant strain was susceptible to several different antibiotics and environmental stresses. |
| GO:0071470 cellular response to osmotic stress | IMP PMID:22146126 Characterization of a novel gene related to antibiotic susce... | MARK AS OVER ANNOTATED | Summary: IMP from mutant osmotic stress susceptibility. Reason: Indirect envelope-integrity phenotype, not a response to osmotic stress. Supporting Evidence: PMID:22146126 We found that the PA0011 mutant strain was susceptible to several different antibiotics and environmental stresses. |
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Download this section (compressed HTML)Experiment: Assay purified HtrB1 with Kdo2-lipid IVA or penta-acylated lipid A and lauroyl- or 2-hydroxylauroyl-ACP to define its acceptor site and donor.
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