PA0692 (PdtB) is the TpsB-family outer-membrane transporter of the PdtA/PdtB two-partner secretion (type Vb) system of Pseudomonas aeruginosa. It has an N-terminal signal peptide, POTRA domains and a C-terminal ShlB/FhaC-type beta-barrel of the Omp85 superfamily, and forms the pore through which its partner PdtA (PA0690), a large HMW-adhesin-like exoprotein made under phosphate limitation, crosses the outer membrane. Export of PdtA strictly depends on PdtB; PdtA contributes to virulence in a Caenorhabditis elegans infection model.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0008320 transmembrane protein transporter activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT IBA from PTN002160772 with WITH/FROM PA4624 (CdrB, Q9HVG7), the TpsB partner of CdrA; PA0692 is not the seed of this row. Reason: PA0692 has the POTRA plus ShlB beta-barrel architecture of TpsB transporters, and its own mutant shows that PdtA transport depends on it. Translocating the partner exoprotein across the outer membrane is the core activity of TpsB proteins, so the node assertion holds for PA0692. Supporting Evidence: PMID:25009238 composed of the PdtA exoprotein and its cognate pore-forming Ξ²-barrel PdtB transporter file:PSEAE/PA0692/PA0692-uniprot.txt Pfam; PF03865; ShlB; 1. |
| GO:0046819 protein secretion by the type V secretion system | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT IBA from PTN002160772, whose IBD for this term rests solely on the PseudoCAP IMP row for PA0692. Reason: The seed is sound (PdtB is the transporter whose loss blocks PdtA export), and the node is a TpsB subfamily whose members are type Vb transporters, so the IBD and this IBA hold. PA0692 in its own WITH/FROM marks its experimental seed and is expected. Supporting Evidence: PMID:25009238 the transport of PdtA is strictly dependent on the production of PdtB |
| GO:0046819 protein secretion by the type V secretion system | IMP PMID:25009238 Characterization of a novel two-partner secretion system imp... | ACCEPT | Summary: PseudoCAP IMP from Faure et al. 2014; PdtA is not exported without PdtB. Sole seed of the PTN002160772 IBD for this term. Reason: PdtB is the cognate beta-barrel transporter, so the mutant phenotype reflects loss of the entity that performs the translocation; IMP is the right code for this process claim. Only the abstract is available, which states the dependency directly. Supporting Evidence: PMID:25009238 We also obtained evidence that the transport of PdtA is strictly dependent on the production of PdtB, a result confirming that these proteins constitute a functional TPS system |
| GO:0098046 type V protein secretion system complex | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT IBA from PTN002160772, whose IBD for this term rests solely on the PseudoCAP IMP CC row for PA0692. Reason: The claim holds by family. GO:0098046 explicitly includes the two-partner secretion system, and TpsB proteins are its beta-barrel component, so descendants of this TpsB node are reasonably annotated part_of it. The seed's evidence code is inappropriate (see the IMP row), but the node conclusion does not depend on it. Supporting Evidence: file:PSEAE/PA0692/PA0692-uniprot.txt InterPro; IPR051544; TPS_OM_transporter. PMID:25009238 composed of the PdtA exoprotein and its cognate pore-forming Ξ²-barrel PdtB transporter |
| GO:0098046 type V protein secretion system complex | IMP PMID:25009238 Characterization of a novel two-partner secretion system imp... | ACCEPT | Summary: PseudoCAP IMP for a complex CC term; sole seed of the PTN002160772 IBD for this term. Reason: The term is correct for PdtB (the TpsB pore of a type Vb system), but a mutant export defect shows requirement, not location or complex membership, so IMP is not a suitable evidence code for this CC claim. The abstract does not say whether PdtB was localized; if the full text includes outer-membrane fractionation the row should be IDA, otherwise IC or ISS. Accepted on the claim, with an evidence-code defect recorded; not removed because the full text was not read. Supporting Evidence: PMID:25009238 composed of the PdtA exoprotein and its cognate pore-forming Ξ²-barrel PdtB transporter |
| GO:0009279 cell outer membrane | IC PMID:25009238 Characterization of a novel two-partner secretion system imp... | NEW | Summary: Proposed outer-membrane location for the TpsB pore of the PdtA/PdtB system. Reason: Two independent lines agree. PdtB carries the POTRA plus ShlB/FhaC beta-barrel architecture of TpsB outer-membrane transporters (InterPro TPS_OM_transporter), and the paper describes it as the pore-forming beta-barrel transporter through which PdtA crosses the outer membrane. IC because no localization assay for PdtB is visible in the abstract. Supporting Evidence: file:PSEAE/PA0692/PA0692-uniprot.txt InterPro; IPR051544; TPS_OM_transporter. PMID:25009238 We show that PdtA is processed during its outer-membrane translocation |
Loading supporting contentβ¦
Download this section (compressed HTML)Q: Does the full text of PMID:25009238 localize PdtB to the outer membrane, which would justify IDA rather than IMP for the type V secretion complex row?
Q: GO:0046819 is defined for autotransporter-style self-secretion; should the definition be broadened to cover two-partner (type Vb) secretion, where a separate TpsB pore exports the TpsA partner?
Loading supporting contentβ¦
Download this section (compressed HTML)Loading supporting contentβ¦
Download this section (compressed HTML)