PA1341 is an integral inner-membrane permease subunit of a polar amino acid ABC importer of Pseudomonas aeruginosa, encoded in the PA1342-PA1339 operon that is orthologous to the AatJQMP acidic amino acid transporter of Pseudomonas putida and to GltIJKL of Escherichia coli. PA1341 is the GltJ/AatQ-type permease (HisMQ subfamily, PANTHER subfamily "glutamate/ aspartate import permease protein GltJ"), with four predicted transmembrane helices. Together with the second permease PA1340, the ATPase PA1339 and the periplasmic binding protein, it imports L-glutamate, and probably L-aspartate and, with lower efficiency, L-glutamine and D-glutamate. Transposon mutants are defective in using L-glutamate as a carbon source, and the operon is induced by L-glutamate.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003333 amino acid transmembrane transport | IEA GO_REF:0000108 | MODIFY | Summary: Inferred from the generic MF row; correct, but the substrate is known. Reason: The supported specific process is L-glutamate import across plasma membrane (GO:0098712), a descendant of amino acid transmembrane transport. Proposed replacements: L-glutamate import across plasma membrane Supporting Evidence: PMID:18833300 All three mutants were defective for utilization of L-glutamic acid as a carbon source, and expression of two of these genes was induced by L-glutamic acid |
| GO:0005886 plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: PA1341 is a polytopic inner (plasma) membrane ABC permease. Reason: Seeded by experimentally localised E. coli HisMQ-subfamily permeases (including GltJ and GltK, located in the plasma membrane by IDA). PA1341 has four predicted TM helices and the ABC transmembrane type-1 domain. Supporting Evidence: file:PSEAE/PA1341/PA1341-uniprot.txt PROSITE; PS50928; ABC_TM1; 1. |
| GO:0005886 plasma membrane | IEA GO_REF:0000120 | ACCEPT | Summary: Inner-membrane location of an ABC permease subunit. Reason: Agrees with the IBA row and with the predicted multi-pass topology. Supporting Evidence: file:PSEAE/PA1341/PA1341-uniprot.txt TRANSMEM 24..50 /note="Helical" |
| GO:0015171 amino acid transmembrane transporter activity | IBA GO_REF:0000033 | MODIFY | Summary: Correct for the HisMQ-subfamily clade; for PA1341 the substrate (L-glutamate) and the ATP coupling are known. Reason: The node term suits a clade of polar amino acid ABC permeases of varied specificity. PA1341 is the GltJ/AatQ permease of an L-glutamate ABC importer, so the specific activity is ATPase-coupled L-glutamate transmembrane transporter activity (GO:0102013, a descendant via ABC-type amino acid transporter activity). As with other ABC permease subunits, the activity belongs to the assembled complex; enables is kept for convention here and contributes_to is used in core_functions. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: GRANULARITY MISMATCH Sources checked: PANTHER:PTN000770157 · PTN000770157 SUPPORTS TRANSFER HisMQ-subfamily polar amino acid permease clade seeded by E. coli permeases; the generic term transfers correctly, but PA1341 supports a more specific substrate. Proposed replacements: ATPase-coupled L-glutamate tranmembrane transporter activity Supporting Evidence: PMID:18833300 PA1339, PA1340 and PA1341 all encode components of a predicted ABC amino acid transporter, and are located in a putative three gene operon. PMID:18310026 We describe an ATP-binding cassette (ABC) transporter in Pseudomonas putida KT2440 that mediates the uptake of glutamate and aspartate. |
| GO:0016020 membrane | IEA GO_REF:0000120 | MODIFY | Summary: Correct but general; the specific membrane is the plasma membrane. Reason: Plasma membrane, an is_a descendant, is already annotated on this gene by IBA and IEA. Proposed replacements: plasma membrane Supporting Evidence: file:PSEAE/PA1341/PA1341-uniprot.txt Pfam; PF00528; BPD_transp_1; 1. |
| GO:0022857 transmembrane transporter activity | IEA GO_REF:0000002 | MODIFY | Summary: Correct but uninformative; the substrate is known. Reason: The specific activity supported for this gene is ATPase-coupled L-glutamate transmembrane transporter activity (GO:0102013). Proposed replacements: ATPase-coupled L-glutamate tranmembrane transporter activity Supporting Evidence: PMID:18833300 All three mutants were defective for utilization of L-glutamic acid as a carbon source |
| GO:0043190 ATP-binding cassette (ABC) transporter complex | IEA GO_REF:0000120 | MODIFY | Summary: PA1341 is a permease subunit of a binding protein-dependent ABC importer. Reason: The orthologous P. putida AatJMQP system depends on its periplasmic solute-binding protein (an aatJ mutant cannot grow on Glu), so the precise complex is the substrate-binding subunit-containing ABC transporter complex (GO:0055052, an is_a descendant), as used for the Dpp permeases. Proposed replacements: ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing Supporting Evidence: PMID:18310026 A deletion mutant with inactivated solute-binding protein (KTaatJ) failed to grow on Glu and Gln as sole sources of carbon and nitrogen |
| GO:0051938 L-glutamate import | IBA GO_REF:0000033 | MODIFY | Summary: IBA from PTN004745596, whose IBD rests solely on PA1341's own PseudoCAP IMP. Correct for PA1341 and for this GltJ clade. Reason: PA1341 in its own WITH/FROM marks its experimental seed and is expected. The node is a GltJ-type permease clade of 7 leaves in 5 taxa (E. coli, Salmonella and Yersinia GltJ, three Bradyrhizobium permeases, PA1341). E. coli GltIJKL is the glutamate/aspartate ABC importer and P. putida AatJMQP takes up Glu and Asp with high affinity, so L-glutamate import suits the clade. For PA1341 the direction and membrane are known, so the precise term is L-glutamate import across plasma membrane (GO:0098712, a descendant via L-glutamate transmembrane transport), as for the IMP row. Propagation Review Root cause: NO FAILURE CORE Sources checked: UniProtKB:Q9I403 · PA1341 (self; sole IBD seed, PseudoCAP IMP PMID:18833300) SUPPORTS TRANSFER Biolog/plate growth screen of a transposon mutant (reduced use of L-Glu as carbon source), with substrate induction of the operon; indirect, but corroborated by the P. putida ortholog's uptake and binding data. PANTHER:PTN004745596 · PTN004745596 SUPPORTS TRANSFER GltJ-type permease clade of glutamate/aspartate ABC importers. Proposed replacements: L-glutamate import across plasma membrane Supporting Evidence: PMID:18833300 All three mutants were defective for utilization of L-glutamic acid as a carbon source, and expression of two of these genes was induced by L-glutamic acid PMID:18310026 Uptake of Glu and Asp by either mutant was greatly impaired at both low and high amino acid concentrations. |
| GO:0051938 L-glutamate import | IMP PMID:18833300 High-throughput phenotypic characterization of Pseudomonas a... | MODIFY | Summary: Johnson et al. 2008 Biolog and plate-growth screen: the PA1341 transposon mutant is partially defective in using L-glutamate as a carbon source, and the operon is induced by L-glutamate. Sound, and refinable to import across the plasma membrane. Reason: A growth defect on a sole carbon source is an indirect read-out of transport, and the PA1341 phenotype is partial (C+/-). It is consistent across all three operon mutants and with substrate induction, and the orthologous P. putida system imports Glu directly, so the claim holds. As a permease, PA1341 forms part of the translocation pathway and so does part of the work. The precise term is L-glutamate import across plasma membrane. Proposed replacements: L-glutamate import across plasma membrane Supporting Evidence: PMID:18833300 PA1339, PA1340 and PA1341 all encode components of a predicted ABC amino acid transporter, and are located in a putative three gene operon. All three mutants were defective for utilization of L-glutamic acid as a carbon source |
| GO:0055085 transmembrane transport | IEA GO_REF:0000002 | MODIFY | Summary: Correct but uninformative for a glutamate ABC permease. Reason: The specific process supported is L-glutamate import across plasma membrane (GO:0098712), a descendant of transmembrane transport. Proposed replacements: L-glutamate import across plasma membrane Supporting Evidence: PMID:18833300 All three mutants were defective for utilization of L-glutamic acid as a carbon source |
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Download this section (compressed HTML)Q: Does the P. aeruginosa PA1342-PA1339 system also take up L-aspartate, as its P. putida ortholog does, and is L-glutamine a genuine low-affinity substrate or used only after periplasmic deamidation?
Experiment: [14C]-L-glutamate and [14C]-L-aspartate uptake in an in-frame PA1341 deletion with complementation, in PAO1, to attribute the operon phenotype to the permease and define its substrate range.
Type: transport assay
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