PA2070

Gene Description

PA2070 (Q9I245) is an uncharacterised TonB-dependent outer membrane receptor of Pseudomonas aeruginosa PAO1 (PANTHER PTHR47234:SF2). It has a signal peptide, a plug domain and a 22-strand beta-barrel. Receptors of this family bind a ligand at the cell surface and, energised by the TonB-ExbB-ExbD complex through the TonB box, translocate it across the outer membrane; many import siderophore-iron complexes or other scarce nutrients. The substrate of this receptor is not known.

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0009279 cell outer membrane	IEA GO_REF:0000120	ACCEPT	Summary: UniProt subcellular location from the TonB-dependent receptor family rule. Reason: The plug and 22-strand TonB-dependent receptor beta-barrel domains define an integral outer-membrane protein of a Gram-negative bacterium; cell outer membrane is the precise term. Supporting Evidence: file:PSEAE/PA2070/PA2070-uniprot.txt PF00593; TonB_dep_Rec_b-barrel; 1. file:PSEAE/PA2070/PA2070-uniprot.txt SIMILARITY: Belongs to the TonB-dependent receptor family.
GO:0019867 outer membrane	IBA GO_REF:0000033	TOO GENERAL	Summary: IBA from the PTN002933024 IBD (22 leaves in P. aeruginosa, Shewanella and Xanthomonas), seeded by four PAO1 receptors including PA2070 itself (expected, not circular), all from the PseudoCAP IDA citing PMID:18086184. Reason: True but entailed: GO:0009279 cell outer membrane, accepted in this review, is_a GO:0019867. The node term suits the clade, whose leaves are all TonB-dependent receptors (BtuB, BfeA and relatives), although its four seeds rest on one abstract-only paper; the node-level assessment is in the PA2590 notes. Supporting Evidence: file:PSEAE/PA2070/PA2070-uniprot.txt PF00593; TonB_dep_Rec_b-barrel; 1.
GO:0019867 outer membrane	IDA PMID:18086184 Characterization of five novel Pseudomonas aeruginosa cell-s...	TOO GENERAL	Summary: PseudoCAP IDA; seed of the PTN002933024 IBD. Seed verdict UNVERIFIABLE (abstract only), but the location is correct on sequence grounds. Reason: The outer-membrane location is right for a TonB-dependent receptor, but GO:0009279 cell outer membrane, accepted in this review, is_a GO:0019867. Whether this paper detected PA2070 in an outer-membrane fraction could not be checked from the abstract, so no defect is recorded. Supporting Evidence: PMID:18086184 This regulatory system consists of an outer membrane-localized TonB-dependent receptor (TonB-dependent transducer) file:PSEAE/PA2070/PA2070-uniprot.txt PF00593; TonB_dep_Rec_b-barrel; 1.

Core Functions

TonB-dependent outer membrane receptor of unknown substrate; by family membership it is expected to bind a ligand at the cell surface and import it across the outer membrane using energy transduced from the TonB-ExbB-ExbD complex.

Cellular Locations:

cell outer membrane

Supporting Evidence:

file:PSEAE/PA2070/PA2070-uniprot.txt
PF00593; TonB_dep_Rec_b-barrel; 1.
file:PSEAE/PA2070/PA2070-uniprot.txt
SIMILARITY: Belongs to the TonB-dependent receptor family.

References

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Suggested Experiments

Experiment: Screen siderophores and other TonB-dependent substrates for growth promotion under iron limitation in a PA2070 deletion mutant versus wild type and complemented strain, and confirm outer-membrane localisation by fractionation of a tagged protein.

Knowledge Gaps

What is not known — curated, literature-grounded statements of the open unknowns (the inverse of core functions).

Gap: The ligand imported or sensed by PA2070 is unknown, as is which ECF sigma factor controls it and whether it signals to an anti-sigma factor.

BIOLOGY