PA2089 (Q9I226) is an uncharacterised TonB-dependent outer membrane receptor of Pseudomonas aeruginosa PAO1 (PANTHER PTHR47234:SF3). It has a signal peptide, an N-terminal secretin/TonB short N-terminal (STN) domain of the kind that marks TonB-dependent transducers of cell-surface signalling systems, a plug domain and a 22-strand beta-barrel. Receptors of this family bind a ligand at the cell surface and, energised by the TonB-ExbB-ExbD complex through the TonB box, translocate it across the outer membrane; many import siderophore-iron complexes or other scarce nutrients. The substrate of this receptor is not known.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0009279 cell outer membrane | IEA GO_REF:0000120 | ACCEPT | Summary: UniProt subcellular location from the TonB-dependent receptor family rule. Reason: The plug and 22-strand TonB-dependent receptor beta-barrel domains define an integral outer-membrane protein of a Gram-negative bacterium; cell outer membrane is the precise term. Supporting Evidence: file:PSEAE/PA2089/PA2089-uniprot.txt PF00593; TonB_dep_Rec_b-barrel; 1. file:PSEAE/PA2089/PA2089-uniprot.txt SIMILARITY: Belongs to the TonB-dependent receptor family. |
| GO:0019867 outer membrane | IBA GO_REF:0000033 | TOO GENERAL | Summary: IBA from the PTN002933024 IBD (22 leaves in P. aeruginosa, Shewanella and Xanthomonas), seeded by four PAO1 receptors including PA2089 itself (expected, not circular), all from the PseudoCAP IDA citing PMID:18086184. Reason: True but entailed: GO:0009279 cell outer membrane, accepted in this review, is_a GO:0019867. The node term suits the clade, whose leaves are all TonB-dependent receptors (BtuB, BfeA and relatives), although its four seeds rest on one abstract-only paper; the node-level assessment is in the PA2590 notes. Supporting Evidence: file:PSEAE/PA2089/PA2089-uniprot.txt PF00593; TonB_dep_Rec_b-barrel; 1. |
| GO:0019867 outer membrane | IDA PMID:18086184 Characterization of five novel Pseudomonas aeruginosa cell-s... | TOO GENERAL | Summary: PseudoCAP IDA; seed of the PTN002933024 IBD. Seed verdict UNVERIFIABLE (abstract only), but the location is correct on sequence grounds. Reason: The outer-membrane location is right for a TonB-dependent receptor, but GO:0009279 cell outer membrane, accepted in this review, is_a GO:0019867. Whether this paper detected PA2089 in an outer-membrane fraction could not be checked from the abstract, so no defect is recorded. Supporting Evidence: PMID:18086184 This regulatory system consists of an outer membrane-localized TonB-dependent receptor (TonB-dependent transducer) file:PSEAE/PA2089/PA2089-uniprot.txt PF00593; TonB_dep_Rec_b-barrel; 1. |
| GO:0019867 outer membrane | IEA GO_REF:0000002 | TOO GENERAL | Summary: InterPro2GO mapping from the STN domain. Reason: GO:0009279 cell outer membrane, accepted in this review, is_a GO:0019867. Supporting Evidence: file:PSEAE/PA2089/PA2089-uniprot.txt IPR011662; Secretin/TonB_short_N. |
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Download this section (compressed HTML)Experiment: Screen siderophores and other TonB-dependent substrates for growth promotion under iron limitation in a PA2089 deletion mutant versus wild type and complemented strain, and confirm outer-membrane localisation by fractionation of a tagged protein.
What is not known — curated, literature-grounded statements of the open unknowns (the inverse of core functions).
Gap: The ligand imported or sensed by PA2089 is unknown, as is which ECF sigma factor controls it and whether it signals to an anti-sigma factor.
BIOLOGY
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