PA2412 is a 72-residue MbtH-like protein encoded in the pyoverdine biosynthesis cluster of Pseudomonas aeruginosa PAO1 and co-expressed with the pyoverdine nonribosomal peptide synthetases. MbtH-like proteins are small cytosolic partners of NRPS adenylation domains; PA2412 has a solved 1.8 A crystal structure, and its deletion lowers pyoverdine production without affecting uptake or use of ferripyoverdine, so it is an accessory component of the pyoverdine NRPS assembly line.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0002049 pyoverdine biosynthetic process | IMP PMID:12207696 GeneChip expression analysis of the iron starvation response... | ACCEPT | Summary: PseudoCAP seed row. The abstract describes an expression study; whether a PA2412 mutant was assayed is not stated. Seed verdict UNVERIFIABLE. The term itself is correct for PA2412 (PMID:17502378). Reason: The claim is true on the deletion-mutant data of PMID:17502378, and MbtH-like proteins are NRPS adenylation-domain partners, so PA2412 is a component of the biosynthetic machinery rather than a regulator. Whether this particular reference supports IMP is unverified. Supporting Evidence: PMID:17502378 Our results demonstrate that PA2412 is necessary for the production or secretion of pyoverdine at normal levels. |
| GO:0002049 pyoverdine biosynthetic process | IMP PMID:12686626 Identification and characterization of novel pyoverdine synt... | ACCEPT | Summary: PseudoCAP seed row. The paper mutated candidate pvd genes and measured pyoverdine; the abstract does not name PA2412. Seed verdict UNVERIFIABLE; the term is correct. Reason: Same claim as the PMID:17502378 row, which is directly supported. Supporting Evidence: PMID:17502378 Our results demonstrate that PA2412 is necessary for the production or secretion of pyoverdine at normal levels. |
| GO:0002049 pyoverdine biosynthetic process | IMP PMID:17502378 The 1.8 A crystal structure of PA2412, an MbtH-like protein ... | ACCEPT | Summary: PseudoCAP seed row. A PA2412 deletion lowers pyoverdine output while uptake and use of ferripyoverdine are normal. Seed verdict SOUND. Reason: Direct mutant evidence with uptake excluded. The abstract leaves production versus secretion open, but PA2412 is co-expressed with and homologous to NRPS-associated MbtH proteins, which supports a role in synthesis. Supporting Evidence: PMID:17502378 Our results demonstrate that PA2412 is necessary for the production or secretion of pyoverdine at normal levels. The PA2412 deletion strain is able to use exogenously produced pyoverdine, showing that there is no defect in the uptake or utilization of the iron-pyoverdine complex. |
| GO:0005829 cytosol | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN002212932, seeded by E. coli YbdZ (IDA cytosol, PMID:18304323). Reason: A small soluble protein without signal peptide or TM segment that binds cytosolic NRPS enzymes. |
| GO:0019290 siderophore biosynthetic process | IBA GO_REF:0000033 | TOO GENERAL | Summary: IBA from PTN002212932 (8 leaf IBAs, 7 taxa), seeded by E. coli YbdZ (IMP/IDA enterobactin biosynthesis) and PA2412 itself (expected, not circular). The IBD stands without this seed. Reason: Entailed by GO:0002049 pyoverdine biosynthetic process (is_a GO:0019290), accepted above. |
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Download this section (compressed HTML)Q: Which pyoverdine NRPS adenylation domains (PvdL, PvdI, PvdJ, PvdD) does PA2412 bind, and is it required for their adenylation activity or only for their solubility?
Experiment: Co-express each pyoverdine NRPS adenylation domain with and without PA2412 and measure soluble yield and amino-acid adenylation (ATP-PPi exchange).
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