PA3677 (mexJ) encodes the membrane fusion protein (periplasmic adaptor) of the MexJK resistance-nodulation-cell division (RND) efflux system of Pseudomonas aeruginosa. It is a predicted inner-membrane lipoprotein whose periplasmic barrel-sandwich and beta-barrel domains link the inner-membrane RND transporter MexK to an outer-membrane channel. MexJ and MexK together are required for triclosan efflux, while efflux of tetracycline and erythromycin also needs the outer-membrane channel OprM, forming a tripartite MexJK-OprM pump. The mexJK operon is repressed by the TetR-family regulator MexL, encoded divergently next to it.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0016020 membrane | IEA GO_REF:0000002 | MODIFY | Summary: InterPro2GO from the RND membrane fusion protein family. MexJ carries a lipoprotein signal peptide (lipidated Cys21), the inner-membrane anchor used by MexA-type MFPs. Reason: Correct but general. The lipoprotein signature and the MFP family architecture agree on an inner (plasma) membrane anchor with a periplasmic body. Proposed replacements: plasma membrane Supporting Evidence: file:PSEAE/PA3677/PA3677-uniprot.txt S-diacylglycerol cysteine |
| GO:0022857 transmembrane transporter activity | IEA GO_REF:0000002 | MARK AS OVER ANNOTATED | Summary: InterPro2GO from the MFP family. The transporter activity of an RND pump resides in the inner-membrane RND protein (MexK); the MFP is a periplasmic adaptor required for the pump to work. Reason: The MFP contributes to the pump's activity but does not enable transmembrane transport alone; at most a contributes_to annotation would be warranted. Supporting Evidence: PMID:12193619 Triclosan efflux required neither of the outer membrane protein channels tested but necessitated the MexJ membrane fusion protein and the MexK inner membrane RND transporter. |
| GO:0046677 response to antibiotic | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: IBA from PTN002427209, seeded by E. coli YbhG (EcoCyc IDA PMID:27112147) and by MexJ itself. The clade is efflux-system membrane fusion proteins. Reason: True resistance phenotype of an efflux component; the core role is xenobiotic export. The self-seed reflects MexJ's own evidence and is expected. Supporting Evidence: PMID:12193619 Triclosan efflux required neither of the outer membrane protein channels tested but necessitated the MexJ membrane fusion protein and the MexK inner membrane RND transporter. |
| GO:0046677 response to antibiotic | IMP PMID:12193619 The MexJK efflux pump of Pseudomonas aeruginosa requires Opr... | KEEP AS NON CORE | Summary: PseudoCAP IMP and a seed of the PTN002427209 IBD. MexJ is required for triclosan efflux, and MexJK-OprM effluxes tetracycline and erythromycin. Reason: Seed verdict SOUND: the paper shows the requirement directly. Non-core relative to the export function, as on the PA4218 efflux transporter review. Supporting Evidence: PMID:12193619 Triclosan efflux required neither of the outer membrane protein channels tested but necessitated the MexJ membrane fusion protein and the MexK inner membrane RND transporter. |
| GO:0055085 transmembrane transport | IEA GO_REF:0000002 | MODIFY | Summary: InterPro2GO from the MFP family; MexJ is part of an efflux pump exporting triclosan and antibiotics. Reason: Correct but general. GO:1990961 xenobiotic detoxification by transmembrane export across the plasma membrane (is_a transmembrane transport via export across plasma membrane) names the process the pump performs. Proposed replacements: xenobiotic detoxification by transmembrane export across the plasma membrane Supporting Evidence: PMID:12193619 The MexJK system effluxed tetracycline and erythromycin but only in the presence of the outer membrane protein channel OprM |
| GO:1990281 efflux pump complex | IC PMID:12193619 The MexJK efflux pump of Pseudomonas aeruginosa requires Opr... | NEW | Summary: Proposed component. MexJ is the membrane fusion protein of the MexJK and tripartite MexJK-OprM pumps. Reason: The paper defines MexJ as a required pump component, and MFP family membership agrees; no complex annotation exists. Supporting Evidence: PMID:12193619 The results presented in this study suggest that MexJK may function as a two-component RND pump for triclosan efflux but must associate with OprM to form a tripartite antibiotic efflux system. |
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