PA3933 encodes BetT2, one of three betaine/carnitine/choline transporter (BCCT, TC 2.A.15) family secondary transporters of Pseudomonas aeruginosa. It is a multi-pass cytoplasmic membrane protein with twelve predicted transmembrane helices and a long C-terminal tail. When expressed in Pseudomonas syringae it is reported to transport glycine betaine and to give osmoprotection at high salt. Unlike betT1 and betT3, betT2 expression does not respond to choline or glycine betaine, and its role in P. aeruginosa itself has not been tested.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005275 amine transmembrane transporter activity | IDA PMID:21478341 Roles of three transporters, CbcXWV, BetT1, and BetT3, in Ps... | REMOVE | Summary: PseudoCAP IDA seed of PTN001247333 citing Malek et al. 2011. Reason: Full text read. The paper reports no transport assay on BetT2 (PA3933); the choline transport data are for BetT1 and BetT3, and BetT3 is PA5291. Wrong gene product. Seed verdict WRONG. Supporting Evidence: file:PSEAE/PA3933/PA3933-notes.md three BCCTs, designated BetT1, BetT2, and BetT3 (PA14_70980, PA14_12990, and PA14_69850, respectively) file:PSEAE/PA5291/PA5291-bioinformatics/RESULTS.md PA14_12990 BetT2 is identical to PAO1 PA3933 |
| GO:0033265 choline binding | IDA PMID:21478341 Roles of three transporters, CbcXWV, BetT1, and BetT3, in Ps... | REMOVE | Summary: PseudoCAP IDA choline binding citing Malek et al. 2011. Reason: Wrong gene product, as for the GO:0005275 row; the paper also has no binding assay for any transporter. Supporting Evidence: file:PSEAE/PA3933/PA3933-notes.md Neither choline nor GB affected levels of betT2 and opuCA transcripts in MOPS-P medium |
| GO:0042426 choline catabolic process | IDA PMID:21478341 Roles of three transporters, CbcXWV, BetT1, and BetT3, in Ps... | REMOVE | Summary: PseudoCAP IDA choline catabolic process citing Malek et al. 2011. Reason: Wrong gene product: the growth-on-choline phenotypes are of betT1 and betT3 mutants, and BetT3 is PA5291. On the right gene this would still be an indirect, uptake-dependent phenotype (see the betT1 and PA5377 reviews). Supporting Evidence: PMID:21478341 BetT3 and CbcXWV, but not BetT1, were sufficient to support growth on choline. |
| GO:0071475 cellular hyperosmotic salinity response | IDA PMID:21478341 Roles of three transporters, CbcXWV, BetT1, and BetT3, in Ps... | REMOVE | Summary: PseudoCAP IDA hyperosmotic salinity response citing Malek et al. 2011. Reason: Wrong gene product: the high-osmolarity data identify BetT3 (PA5291) as the main choline transporter under these conditions; BetT2 was not tested. Supporting Evidence: PMID:21478341 BetT3 was the major choline transporter under hyperosmolar conditions |
| GO:0015837 amine transport | IEA GO_REF:0000108 | REMOVE | Summary: Inferred from the GO:0005275 IDA row. Reason: Rests only on the wrong-gene GO:0005275 row removed here; glycine betaine, the substrate reported for BetT2, is not an amine in GO's sense either. Supporting Evidence: file:PSEAE/PA3933/PA3933-notes.md Neither choline nor GB affected levels of betT2 and opuCA transcripts in MOPS-P medium |
| GO:0022857 transmembrane transporter activity | IBA GO_REF:0000033 | ACCEPT | Summary: PAINT transmembrane transporter IBD at the BCCT family node PTN001247333. Reason: The IBD stands without the wrong PA3933 seed, on E. coli BetT and CaiT and on BetT1 and BetT3. PA3933 is a complete BCCT and is reported to transport glycine betaine, so the family term transfers. A substrate-specific term is not used because that report could not be read in full. Supporting Evidence: file:PSEAE/PA3933/PA3933-uniprot.txt TIGR00842; bcct file:PSEAE/PA3933/PA3933-notes.md We previously showed that BetT2 and BetT3 transport GB and choline, respectively |
| GO:0022857 transmembrane transporter activity | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro2GO transmembrane transporter activity from IPR000060 (BCCT). Reason: As for the IBA row. Supporting Evidence: file:PSEAE/PA3933/PA3933-uniprot.txt TIGR00842; bcct |
| GO:0055085 transmembrane transport | IEA GO_REF:0000108 | ACCEPT | Summary: Inferred from transmembrane transporter activity. Reason: As precise as the MF term accepted here. Supporting Evidence: file:PSEAE/PA3933/PA3933-uniprot.txt TIGR00842; bcct |
| GO:0005886 plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: IBA plasma membrane from the BCCT node, seeded by E. coli BCCTs. Reason: BCCT transporters are multi-pass cytoplasmic membrane proteins; PA3933 has twelve predicted TM helices. Supporting Evidence: file:PSEAE/PA3933/PA3933-uniprot.txt TIGR00842; bcct |
| GO:0005886 plasma membrane | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt subcellular location mapping (cell membrane). Reason: Agrees with the BCCT family location. Supporting Evidence: file:PSEAE/PA3933/PA3933-uniprot.txt TIGR00842; bcct |
| GO:0016020 membrane | IEA GO_REF:0000002 | MODIFY | Summary: InterPro2GO membrane from IPR000060. Reason: True but general; the cytoplasmic (plasma) membrane is accepted on two rows here. Proposed replacements: plasma membrane Supporting Evidence: file:PSEAE/PA3933/PA3933-uniprot.txt TIGR00842; bcct |
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Download this section (compressed HTML)Q: Is BetT2 (PA3933) a glycine betaine:proton symporter, as the 2008 heterologous-expression work is summarised, and does it contribute to glycine betaine uptake or osmoprotection in P. aeruginosa?
Experiment: Radiolabelled glycine betaine and choline uptake by PA3933 expressed in a BCCT/ABC-deficient E. coli (MKH13), and growth of a P. aeruginosa betT2 deletion in a cbcV background on glycine betaine at low and high osmolarity.
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