PA4506 (DppF) is an ATP-binding subunit of the DppBCDF dipeptide ABC importer of Pseudomonas aeruginosa. With the second ATPase DppD and the permeases DppB and DppC it forms the inner-membrane transporter, which takes up a broad range of di- and tripeptides delivered by five periplasmic substrate-binding proteins (DppA1-A5). The ATPase subunits sit on the cytoplasmic face of the inner membrane and couple ATP hydrolysis to transport. In strain PA14 deletion of the dppBCDF operon abolished use of most dipeptides as nitrogen sources, making Dpp the main dipeptide uptake system.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000166 nucleotide binding | IEA GO_REF:0000120 | TOO GENERAL | Summary: InterPro/UniRule nucleotide binding. Reason: Entailed by the accepted ATP binding row. Supporting Evidence: file:PSEAE/PA4506/PA4506-uniprot.txt PF00005; ABC_tran |
| GO:0005524 ATP binding | IEA GO_REF:0000120 | ACCEPT | Summary: InterPro/UniRule ATP binding from the ABC ATPase domain. Reason: Walker A/B ABC domain of the energizing subunit. Supporting Evidence: file:PSEAE/PA4506/PA4506-uniprot.txt PF00005; ABC_tran PMID:25338022 two encoding the hydrophilic nucleotide- and ATP-binding proteins PA14_58470 (dppD) and PA14_58490 (dppF) |
| GO:0005886 plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: IBA plasma membrane from PTN001952620 (E. coli GsiA, OppF and Salmonella OppF seeds). Reason: ABC ATPase subunits are bound to the cytoplasmic face of the inner membrane through the permeases. Supporting Evidence: PMID:25338022 two encoding the hydrophilic nucleotide- and ATP-binding proteins PA14_58470 (dppD) and PA14_58490 (dppF) |
| GO:0005886 plasma membrane | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt subcellular location mapping (inner membrane, peripheral). Reason: Same claim as the IBA row. Supporting Evidence: PMID:25338022 two encoding the hydrophilic nucleotide- and ATP-binding proteins PA14_58470 (dppD) and PA14_58490 (dppF) |
| GO:0015833 peptide transport | IEA GO_REF:0000002 | TOO GENERAL | Summary: InterPro2GO peptide transport (oligo_HPY domain). Reason: Entailed by the dipeptide transmembrane transport row. Supporting Evidence: file:PSEAE/PA4506/PA4506-uniprot.txt TIGR01727; oligo_HPY |
| GO:0016887 ATP hydrolysis activity | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro2GO ATP hydrolysis activity. Reason: Energizing ATPase of the importer. Supporting Evidence: file:PSEAE/PA4506/PA4506-uniprot.txt PF00005; ABC_tran |
| GO:0022857 transmembrane transporter activity | IBA GO_REF:0000033 | TOO GENERAL | Summary: IBA from PTN001952620 (peptide/opine ABC ATPase clade, 106 leaves in 40 taxa), seeded by E. coli GsiA, SapF, DppF, OppF and PA4506 itself. Reason: True, but entailed by the accepted GO:0071916 dipeptide transmembrane transporter activity row (is_a GO:0022857). The broad node term suits a clade whose members move glutathione, putrescine and peptides, and the IBD stands on the E. coli seeds. Supporting Evidence: PMID:25338022 two encoding the hydrophilic nucleotide- and ATP-binding proteins PA14_58470 (dppD) and PA14_58490 (dppF) PMID:25338022 Numerous dipeptides could not be used as N-source by the mutant lacking the DppBCDF transporter system |
| GO:0035442 dipeptide transmembrane transport | IEA GO_REF:0000108 | ACCEPT | Summary: Inferred from the MF row. Reason: Dpp is the main dipeptide uptake system. Supporting Evidence: PMID:25338022 Numerous dipeptides could not be used as N-source by the mutant lacking the DppBCDF transporter system PMID:25338022 The protein sequences from PAO1 are homologous to the following proteins from PA14: DppBCDF (PA4503–PA4506) |
| GO:0055085 transmembrane transport | IEA GO_REF:0000108 | TOO GENERAL | Summary: Inferred from the IBA MF row. Reason: Entailed by dipeptide transmembrane transport. Supporting Evidence: PMID:25338022 Numerous dipeptides could not be used as N-source by the mutant lacking the DppBCDF transporter system |
| GO:0071916 dipeptide transmembrane transporter activity | IDA PMID:25338022 High-throughput screening of dipeptide utilization mediated ... | ACCEPT | Summary: PseudoCAP IDA and one of five seeds of PTN001952620. Pletzer et al. deleted the whole dppBCDF operon in strain PA14 and showed loss of growth on many dipeptides; the paper maps the operon to PAO1 PA4503-PA4506. Reason: Seed verdict SOUND for the term: DppF is the energizing subunit of the dipeptide importer. The evidence is a deletion-mutant growth phenotype in PA14, so the code should be IMP rather than IDA; a source defect records this. Supporting Evidence: PMID:25338022 In order to investigate the substrate profile of the ABC transporter DppBCDF, a deletion mutant of the dppBCDF operon was constructed PMID:25338022 Numerous dipeptides could not be used as N-source by the mutant lacking the DppBCDF transporter system PMID:25338022 The protein sequences from PAO1 are homologous to the following proteins from PA14: DppBCDF (PA4503–PA4506) |
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