PA5208 is an uncharacterised cytoplasmic protein of the UPF0111/TIGR00153 (DUF47) family, which has a PhoU-like helical-bundle fold. Members of the family are often encoded next to Pit-type inorganic phosphate transporters, and PA5208 lies beside the Pit-family gene PA5207, suggesting a role as a PhoU-like regulator or accessory protein of phosphate uptake; this has not been tested. Its only reported phenotype is from a transposon screen, in which a PA5208 insertion mutant grew slightly more slowly and swarmed and formed biofilm less well than wild type.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0044010 single-species biofilm formation | IMP PMID:17158671 Identification of genes involved in swarming motility using ... | MARK AS OVER ANNOTATED | Summary: PseudoCAP IMP from the Overhage et al. 2007 swarming screen: the PA5208 insertion mutant, like most non-flagellar swarming mutants, made modestly less biofilm. Reason: A secondary phenotype of a transposon mutant that also has a growth defect. PA5208 is a PhoU-like protein of unknown activity with no link to matrix production or attachment; reduced biofilm here is necessity evidence at most, and may reflect slower growth or a polar effect on the adjacent Pit transporter gene PA5207. Supporting Evidence: PMID:17158671 and a PA5208 mutant that had a slight growth defect PMID:17158671 all but five of the non-flagellum- or non-pilus-related mutants showed a statistically significant |
| GO:0071978 bacterial-type flagellum-dependent swarming motility | IBA GO_REF:0000033 | REMOVE | Summary: PAINT IBA from PTN002194937, sole seed the PseudoCAP IMP on this protein. Reason: The seed is a screen phenotype, and the node propagates a flagellum-dependent motility term to leaves UniProtKB:P44271 (HI_1603) and UniProtKB:Q83FC5 (CBU_0015), whose organisms encode none of the six core flagellar structural and motor genes fliF, flgB, flgE, flhA, fliC and motA (UniProt proteome query, 2026-11-06), while the flagellated leaves of the same node (PAO1, Shewanella oneidensis) encode all six. The family's own function is only predicted (Pfam PF01865, InterPro IPR018445 "putative phosphate transport regulator"), but it contributes no flagellar component or motor domain. Over-propagated IBA with no valid seed. Propagation Review Root cause: SOURCE BAD Failure modes: ROLE CONFLATION LINEAGE OR TAXON MISMATCH Sources checked: PANTHER:PTN002194937 · PTN002194937 SOURCE BAD PAINT leaf GAF (cached 2026-10-28) lists 5 GO:0071978 leaves, PA5208, SO_3770, VC_2441, HI_1603 and CBU_0015, all confirmed in the PANTHER tree. Haemophilus influenzae Rd KW20 and Coxiella burnetii RSA 493 encode none of fliF, flgB, flgE, flhA, fliC or motA (UniProt proteome query, 2026-11-06), so two of the five leaves cannot perform a flagellum-dependent process. UniProtKB:Q9HTY2 · PA5208 SOURCE BAD Sole seed; PseudoCAP IMP from a transposon screen phenotype (PMID:17158671), not participation. Supporting Evidence: file:PSEAE/PA5208/PA5208-uniprot.txt InterPro; IPR018445; Put_Phosphate_transp_reg. PMID:17158671 and a PA5208 mutant that had a slight growth defect |
| GO:0071978 bacterial-type flagellum-dependent swarming motility | IMP PMID:17158671 Identification of genes involved in swarming motility using ... | MARK AS OVER ANNOTATED | Summary: PseudoCAP IMP from the Overhage et al. 2007 mini-Tn5-lux screen: a PA5208 insertion mutant swarms poorly. Reason: The phenotype is real but indirect: swarming is performed by the flagellum with rhamnolipid surfactant, and PA5208 is a cytoplasmic PhoU-like protein of unknown activity whose mutant also has a growth defect. The insertion may also be polar on the neighbouring Pit gene PA5207. Necessity in a screen does not show participation. Supporting Evidence: PMID:17158671 36 mutants were identified with Tn5 insertions in genes for the synthesis or function of flagellin and type IV pilus, in genes for the Xcp-related type II secretion system, and in regulatory, metabolic, chemosensory, and hypothetical genes with unknown functions. PMID:17158671 and a PA5208 mutant that had a slight growth defect |
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Download this section (compressed HTML)Q: Is PA5208 a PhoU-like regulator of the adjacent Pit transporter PA5207, and does its loss perturb phosphate uptake or polyphosphate levels?
Experiment: Make an in-frame, non-polar PA5208 deletion and test growth, swarming and 32P-phosphate uptake at low and high phosphate, with and without PA5207, to separate a phosphate-transport role from polar or growth effects.
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