AmpG (PA4393) is the muropeptide permease of Pseudomonas aeruginosa, a 14-helix inner-membrane transporter of the major facilitator superfamily (AmpG/SLC33 family, TC 2.A.1.25). By homology to Escherichia coli AmpG, which imports GlcNAc-1,6-anhydro-MurNAc and its peptide derivatives from the periplasm, it returns peptidoglycan turnover products to the cytoplasm for cell-wall recycling. These muropeptides are the signal for AmpR-dependent induction of the chromosomal AmpC beta-lactamase, so ampG inactivation strongly reduces intrinsic beta-lactam resistance and abolishes the high-level resistance of ampD mutants. PA4393 complements an E. coli ampG mutant, whereas its distant paralog PA4218 (AmpP) does not.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005886 plasma membrane | IEA GO_REF:0000044 | ACCEPT | Summary: UniProt subcellular location mapping (cell membrane). Reason: PhoA/LacZ fusions show a 14-helix inner-membrane protein. Supporting Evidence: PMID:21192796 suggesting that AmpG has only 14 TM helices (Figures 4C and 4D). |
| GO:0008521 acetyl-CoA transmembrane transporter activity | IEA GO_REF:0000002 | REMOVE | Summary: InterPro2GO from IPR024371 (AcetylCoA_trans_1-like). Reason: Acetyl-CoA transport is the function of the animal SLC33A1 branch of this family. Bacterial AmpG transports muropeptides; PA4393 complements an E. coli ampG mutant. The InterPro2GO mapping over-propagates the animal substrate to bacterial members. Supporting Evidence: PMID:20713660 The cloned ampG gene is able to complement both the P. aeruginosa and the E. coli ampG mutants |
| GO:0009276 Gram-negative-bacterium-type cell wall | IMP PMID:20713660 ampG gene of Pseudomonas aeruginosa and its role in Ξ²-lactam... | MODIFY | Summary: PseudoCAP row. GO:0009276 is the peptidoglycan layer of the Gram-negative envelope. AmpG is an integral inner-membrane permease. Reason: The location is the plasma (inner) membrane, which the gene already carries; a 14-helix permease is not part of the peptidoglycan layer. Proposed replacements: plasma membrane Supporting Evidence: PMID:21192796 suggesting that AmpG has only 14 TM helices (Figures 4C and 4D). |
| GO:0016020 membrane | IBA GO_REF:0000033 | TOO GENERAL | Summary: IBA from PTN008542531, seeded by human SLC33A1, mouse Slc33a1 and E. coli AmpG. Reason: Entailed by the accepted GO:0005886 plasma membrane (is_a GO:0016020). |
| GO:0016020 membrane | IEA GO_REF:0000002 | TOO GENERAL | Summary: InterPro2GO membrane. Reason: Entailed by the accepted GO:0005886 plasma membrane. |
| GO:0022857 transmembrane transporter activity | IBA GO_REF:0000033 | MODIFY | Summary: IBA from PTN008542531, seeded by human SLC33A1 (O00400, IDA acetyl-CoA transport), E. coli AmpG (P0AE16, IDA peptidoglycan transport) and the PseudoCAP rows of PA4393 and PA4218 (expected self-appearance). Sound at the node. Reason: Correct but general. PA4393 complements the E. coli ampG mutant, so its substrate is the muropeptide class transported by E. coli AmpG; the specific term is peptidoglycan transmembrane transporter activity. Propagation Review Root cause: NO FAILURE CORE Failure modes: GRANULARITY MISMATCH Sources checked: PANTHER:PTN008542531 Β· PTN008542531 SUPPORTS TRANSFER SLC33/AmpG family node spanning bacteria and animals with different substrates; the generic transporter term is the right node level. UniProtKB:P0AE16 Β· E. coli AmpG SUPPORTS TRANSFER IDA peptidoglycan (muropeptide) transmembrane transporter activity. UniProtKB:O00400 Β· human SLC33A1 SUPPORTS SOURCE BUT NOT TARGET IDA acetyl-CoA transporter; supports the generic node term only. UniProtKB:G3XCT7 Β· ampG (self; PseudoCAP IMP PMID:20713660) SUPPORTS TRANSFER Expected self-seed reflecting the target's own complementation evidence. Proposed replacements: peptidoglycan transmembrane transporter activity Supporting Evidence: PMID:20713660 The cloned ampG gene is able to complement both the P. aeruginosa and the E. coli ampG mutants |
| GO:0022857 transmembrane transporter activity | IMP PMID:20713660 ampG gene of Pseudomonas aeruginosa and its role in Ξ²-lactam... | MODIFY | Summary: PseudoCAP seed row for PTN008542531. The abstract reports that cloned PA4393 complements P. aeruginosa and E. coli ampG mutants. Seed verdict SOUND for the generic transporter term. Reason: The claim is sound; complementation of E. coli ampG supports the specific muropeptide (peptidoglycan) transporter term. Proposed replacements: peptidoglycan transmembrane transporter activity Supporting Evidence: PMID:20713660 The cloned ampG gene is able to complement both the P. aeruginosa and the E. coli ampG mutants |
| GO:0033252 regulation of beta-lactamase activity | IMP PMID:20713660 ampG gene of Pseudomonas aeruginosa and its role in Ξ²-lactam... | REMOVE | Summary: PseudoCAP row. ampG mutants fail to induce beta-lactamase; in the companion study the ampC promoter is no longer induced. Reason: AmpG imports the muropeptide signal that leads to ampC transcription; it does not modulate beta-lactamase activity. The effect is on AmpC expression, upstream and indirect. Supporting Evidence: PMID:21192796 Benzyl-penicillin dependent induction of PampC-lacZ was lost in PAOampG or PAOampP (Figure 6). |
| GO:0035348 acetyl-CoA transmembrane transport | IEA GO_REF:0000002 | REMOVE | Summary: InterPro2GO from IPR024371. Reason: Same over-propagated animal substrate as the GO:0008521 row; AmpG is a muropeptide permease. |
| GO:0046677 response to antibiotic | IMP PMID:20713660 ampG gene of Pseudomonas aeruginosa and its role in Ξ²-lactam... | KEEP AS NON CORE | Summary: PseudoCAP row; ampG inactivation sharply lowers intrinsic beta-lactam resistance. Reason: True in the sense that AmpG is needed for beta-lactam-induced AmpC expression, but a downstream consequence of its transport role. Supporting Evidence: PMID:20713660 inactivation of ampG drastically repressed the intrinsic Ξ²-lactam resistance |
Loading supporting contentβ¦
Download this section (compressed HTML)Loading supporting contentβ¦
Download this section (compressed HTML)Loading supporting contentβ¦
Download this section (compressed HTML)