cyaA (PA5272) encodes the class I adenylate cyclase of Pseudomonas aeruginosa, a 950-residue cytoplasmic protein with the four-part class I adenylate cyclase architecture (N-terminal catalytic domain plus second, third and fourth regulatory regions) shared with Escherichia coli CyaA. It is one of three adenylate cyclases in P. aeruginosa, alongside the membrane class III enzyme CyaB and the secreted class II toxin ExoY. CyaA converts ATP to cAMP, which binds the global regulator Vfr; CyaB provides the majority of intracellular cAMP, and CyaA makes a minor contribution whose physiological role is not yet known.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0004016 adenylate cyclase activity | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN002215143 (class I adenylate cyclase clade, PTHR38760, 7 leaf IBAs in 7 taxa). Two seeds - E. coli CyaA (P00936, IDA) and this gene (PseudoCAP IMP to PMID:12586068). Reason: Core enzymatic activity. The IBD stands on the E. coli CyaA seed alone, the prototype class I adenylate cyclase with an IDA activity annotation; PA CyaA carries the same class I catalytic domain (Pfam PF01295). Propagation Review Root cause: NO FAILURE CORE Sources checked: PANTHER:PTN002215143 · PTN002215143 SUPPORTS TRANSFER Class I adenylate cyclase clade; the node term suits every member. UniProtKB:P00936 · E. coli CyaA SUPPORTS TRANSFER IDA seed for GO:0004016 (EcoCyc, QuickGO 2026-11-05). UniProtKB:Q9HTS8 · cyaA (self; PseudoCAP IMP PMID:12586068) SUPPORTS TRANSFER Expected self-seed. The cached abstract does not separate cyaA from cyaB, so the gene-specific IMP could not be checked; the IBD does not depend on it. Supporting Evidence: file:PSEAE/cyaA/cyaA-uniprot.txt Adenylate cyclase class-I N-terminal PMID:22226839 The two other P. aeruginosa ACs, CyaA and CyaB, belong to Class I and III, respectively. |
| GO:0004016 adenylate cyclase activity | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro2GO from the class I adenylate cyclase signature IPR000274. Reason: Correct mapping; agrees with the IBA and the family placement. Supporting Evidence: file:PSEAE/cyaA/cyaA-uniprot.txt Adenylate_cyclase_1 |
| GO:0004016 adenylate cyclase activity | IMP PMID:12586068 Coordinate regulation of bacterial virulence genes by a nove... | ACCEPT | Summary: PseudoCAP seed row for PTN002215143. The abstract describes mutants unable to synthesise cAMP; it does not say which adenylate cyclase genes were deleted, and the full text (Dev Cell 2003, not in PMC) could not be read. Topal et al. 2012 state that CyaB makes most intracellular cAMP and that the role of CyaA is unknown. Reason: The function is clearly correct for the gene (class I catalytic domain, orthology to E. coli CyaA). The gene-specific genetic evidence the curator coded is likely the cyaA cyaB double mutant, which the cached abstract cannot confirm; deferred to the curator (seed verdict UNVERIFIABLE, no defect). Supporting Evidence: PMID:12586068 Transcriptome analysis of mutants lacking the ability to synthesize cAMP or the cAMP binding protein Vfr |
| GO:0006171 cAMP biosynthetic process | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro2GO from IPR000274. Reason: The enzyme catalyses the single step of cAMP synthesis from ATP. Supporting Evidence: file:PSEAE/cyaA/cyaA-uniprot.txt Adenylate_cyclase_1 |
| GO:0006171 cAMP biosynthetic process | IMP PMID:12586068 Coordinate regulation of bacterial virulence genes by a nove... | ACCEPT | Summary: PseudoCAP seed row for the GO:0006171 IBD at PTN002215143 (IBD dated 2026-06-16; the matching leaf IBA is not yet in this gene's GOA file). Same paper and same limitation as the MF row. Reason: Correct for the gene; seed verdict UNVERIFIABLE because the abstract does not name cyaA. The IBD also rests on E. coli CyaA (IMP, PMID:4310317). Supporting Evidence: PMID:12586068 Transcriptome analysis of mutants lacking the ability to synthesize cAMP or the cAMP binding protein Vfr PMID:22226839 CyaB activity indeed accounts for the majority of intracellular cAMP |
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Download this section (compressed HTML)Q: Under which growth conditions does CyaA, rather than CyaB, contribute to the cAMP pool, and is CyaA activity regulated by the PTS (EIIA-Glc) as in E. coli, given that P. aeruginosa lacks glucose PTS-mediated catabolite repression?
Experiment: Purify PA CyaA and measure ATP-to-cAMP conversion kinetics; compare cAMP levels in cyaA, cyaB and cyaA cyaB mutants across carbon sources and oxygen levels.
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