DgcP (PA5487) is a soluble GGEEF-domain diguanylate cyclase conserved across Pseudomonas species that synthesises cyclic di-GMP from two GTP. By similarity to the PA14 protein it is recruited to the cell poles by the inner-membrane protein FimV, where it raises local c-di-GMP. DgcP promotes biofilm formation and exopolysaccharide production and represses motility; its loss reduces virulence in a mouse acute lung injury model, and overexpression shifts the cell from type III towards type VI secretion.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005886 plasma membrane | IBA GO_REF:0000033 | KEEP AS NON CORE | Summary: IBA from PTN002895351, seeded by E. coli transmembrane DGCs. DgcP has no TM segment, so the seeds' reason for a membrane location does not apply; but by similarity to PA14 DgcP it is recruited to the poles by the inner-membrane protein FimV, a peripheral membrane location. Reason: Plausible for DgcP as a peripheral, FimV-dependent location (by similarity only), and peripheral to the enzymatic function. The node-level GO_CENTRAL defect for the PM IBD is recorded once, on wspR. Supporting Evidence: file:PSEAE/dgcP/dgcP-uniprot.txt Localizes at the cell poles through interaction with FimV where it increases the local pools of c-di-GMP |
| GO:0005886 plasma membrane | IEA GO_REF:0000044 | KEEP AS NON CORE | Summary: Mapped from the UniProt location "Cell inner membrane" (by similarity to PA14 DgcP). Reason: Same claim as the IBA and ISS rows; peripheral polar location. Supporting Evidence: file:PSEAE/dgcP/dgcP-uniprot.txt Localizes at the cell poles through interaction with FimV where it increases the local pools of c-di-GMP |
| GO:0005886 plasma membrane | ISS GO_REF:0000024 | KEEP AS NON CORE | Summary: ISS from PA14 DgcP (A0A0H2ZJS2), which localizes to the poles via FimV. Reason: The PA14 and PAO1 proteins are orthologs within one species; a peripheral inner-membrane location is reasonable. Supporting Evidence: file:PSEAE/dgcP/dgcP-uniprot.txt Localizes at the cell poles through interaction with FimV where it increases the local pools of c-di-GMP |
| GO:0043709 cell adhesion involved in single-species biofilm formation | IBA GO_REF:0000033 | MARK AS OVER ANNOTATED | Summary: IBA from PTN002895351 with E. coli DgcZ as sole seed (itself IMP, acts_upstream_of_or_within). DgcP promotes biofilm formation through c-di-GMP; no attachment-specific data exist for it. Reason: A DGC regulates adhesion rather than performing it. The node-level GO_CENTRAL defect is recorded once, on wspR. Propagation Review Root cause: TERM SCOPING PROBLEM Failure modes: ROLE CONFLATION Sources checked: UniProtKB:P31129 · E. coli DgcZ (sole seed) SOURCE WEAK OR INFERRED Seed annotation is IMP with qualifier acts_upstream_of_or_within (PMID:19460094). Supporting Evidence: PMID:25809128 deletion of the dgcP gene revealed that DgcP negatively regulates motility and positively controls biofilm formation in both the olive tree pathogen P. savastanoi pv. savastanoi and the human opportunistic pathogen Pseudomonas aeruginosa |
| GO:0052621 diguanylate cyclase activity | EXP PMID:25809128 Diguanylate cyclase DgcP is involved in plant and human Pseu... | ACCEPT | Summary: UniProt experimental row from the DgcP characterisation paper (abstract only). Reason: Term correct; the curated UniProt function statement cites this paper and the activity is independently shown for PA5487 in PMID:16477007. Supporting Evidence: file:PSEAE/dgcP/dgcP-uniprot.txt Catalyzes the synthesis of cyclic-di-GMP (c-di-GMP) via the condensation of 2 GTP molecules |
| GO:0052621 diguanylate cyclase activity | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from the PTN002895344 IBD (19 seeds, including DgcP itself, which is expected). The IBD stands on many independent seeds. Reason: Core activity. Supporting Evidence: file:PSEAE/dgcP/dgcP-notes.md PA5487 GGEEF +(109) |
| GO:0052621 diguanylate cyclase activity | IDA PMID:16477007 Analysis of Pseudomonas aeruginosa diguanylate cyclases and ... | ACCEPT | Summary: PseudoCAP seed row. Cells overexpressing PA5487 accumulated 109 pmol c-di-GMP per mg wet cell weight (Table 1; full text read on PMC). Seed verdict SOUND. Reason: Direct measurement of c-di-GMP produced by DgcP in vivo. Supporting Evidence: file:PSEAE/dgcP/dgcP-notes.md PA5487 GGEEF +(109) |
| GO:0052621 diguanylate cyclase activity | IEA GO_REF:0000003 | ACCEPT | Summary: EC 2.7.7.65 mapping. Reason: Consistent with the experimental rows. Supporting Evidence: file:PSEAE/dgcP/dgcP-notes.md PA5487 GGEEF +(109) |
| GO:1902201 negative regulation of bacterial-type flagellum-dependent cell motility | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN002895351; gene-level support from the hypermotile dgcP deletion. Reason: DgcP negatively regulates motility in P. aeruginosa and P. savastanoi. Supporting Evidence: PMID:25809128 deletion of the dgcP gene revealed that DgcP negatively regulates motility and positively controls biofilm formation in both the olive tree pathogen P. savastanoi pv. savastanoi and the human opportunistic pathogen Pseudomonas aeruginosa |
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