LpxC (PA4406, EnvA) is the zinc-dependent UDP-3-O-[(R)-3-hydroxyacyl]-N-acetylglucosamine deacetylase of Pseudomonas aeruginosa. It removes the acetyl group from UDP-3-O-acyl-GlcNAc, the second and committed step of lipid A (Raetz pathway) biosynthesis, and so is required for lipopolysaccharide and outer membrane synthesis. The recombinant enzyme is soluble, sensitive to EDTA and reactivated by divalent metal. LpxC is essential and a major target of antibacterial inhibitor development.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005737 cytoplasm | IBA GO_REF:0000033 | ACCEPT | Summary: IBA cytoplasm from PTN002134692, seeded by E. coli LpxC and Arabidopsis LpxC. Reason: LpxC is a soluble cytoplasmic enzyme in bacteria, consistent with its expression as a soluble protein; the mitochondrial location of the plant seeds does not apply here. Supporting Evidence: PMID:9068651 this construct yielded active protein to approximately 18% of the soluble fraction |
| GO:0009245 lipid A biosynthetic process | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN002134692, seeded by E. coli LpxC (P0A725) and this gene. Reason: LpxC catalyses the committed step of lipid A biosynthesis; the IBD stands on E. coli LpxC. Supporting Evidence: file:PSEAE/lpxC/lpxC-uniprot.txt the committed step in lipid A biosynthesis |
| GO:0009245 lipid A biosynthetic process | IDA PMID:9068651 Cloning, expression, and purification of UDP-3-O-acyl-GlcNAc... | ACCEPT | Summary: PseudoCAP IDA seed of PTN002134692. Reason: The paper assayed and purified the deacetylase that performs the committed step of lipid A biosynthesis; seed verdict SOUND. Supporting Evidence: file:PSEAE/lpxC/lpxC-uniprot.txt the committed step in lipid A biosynthesis PMID:9068651 this construct yielded active protein to approximately 18% of the soluble fraction |
| GO:0009245 lipid A biosynthetic process | IEA GO_REF:0000120 | ACCEPT | Summary: UniRule mapping. Reason: As for the IBA row. Supporting Evidence: file:PSEAE/lpxC/lpxC-uniprot.txt the committed step in lipid A biosynthesis |
| GO:0103117 UDP-3-O-acyl-N-acetylglucosamine deacetylase activity | EXP PMID:9068651 Cloning, expression, and purification of UDP-3-O-acyl-GlcNAc... | ACCEPT | Summary: UniProt EXP from the purified recombinant enzyme. Reason: Core activity. Supporting Evidence: file:PSEAE/lpxC/lpxC-uniprot.txt the committed step in lipid A biosynthesis PMID:9068651 this construct yielded active protein to approximately 18% of the soluble fraction |
| GO:0103117 UDP-3-O-acyl-N-acetylglucosamine deacetylase activity | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN002134692 (E. coli, Aquifex, Arabidopsis and this gene). Reason: Core activity, conserved in the family. Supporting Evidence: file:PSEAE/lpxC/lpxC-uniprot.txt the committed step in lipid A biosynthesis |
| GO:0103117 UDP-3-O-acyl-N-acetylglucosamine deacetylase activity | IEA GO_REF:0000120 | ACCEPT | Summary: UniRule mapping. Reason: Core activity. Supporting Evidence: file:PSEAE/lpxC/lpxC-uniprot.txt the committed step in lipid A biosynthesis |
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