LysA (PA5277) is the diaminopimelate decarboxylase of Pseudomonas aeruginosa, a pyridoxal 5'-phosphate-dependent fold-type III decarboxylase of the Orn/Lys/Arg decarboxylase class-II family (LysA subfamily). It decarboxylates meso-2,6-diaminopimelate to L-lysine, the final step of the diaminopimelate pathway of lysine biosynthesis. The cloned gene complements lysine auxotrophs of P. aeruginosa and Escherichia coli and is expressed constitutively.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0003824 catalytic activity | IEA GO_REF:0000002 | TOO GENERAL | Summary: Root MF term from InterPro2GO. Reason: Entailed by GO:0008836 diaminopimelate decarboxylase activity, accepted below. |
| GO:0008836 diaminopimelate decarboxylase activity | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN000159906, seeded by E. coli, M. tuberculosis and M. jannaschii LysA (all with experimental DAPDC annotations) plus the PseudoCAP IMP on P19572 itself (expected, not circular). The IBD stands without this seed. Reason: Core activity of a LysA-subfamily DAPDC. Supporting Evidence: PMID:3018430 This gene complemented a lys mutation at the lys-12 locus of P. aeruginosa and a lysA defect in Escherichia coli. |
| GO:0008836 diaminopimelate decarboxylase activity | IEA GO_REF:0000120 | ACCEPT | Summary: Combined IEA (InterPro, UniRule, EC) for DAPDC activity. Reason: Agrees with the experimental and phylogenetic rows. |
| GO:0008836 diaminopimelate decarboxylase activity | IMP PMID:3018430 Heterologous expression and regulation of the lysA genes of ... | ACCEPT | Summary: PseudoCAP seed row. The abstract identifies the cloned gene as encoding DAP-decarboxylase and shows it complements lysA mutants of P. aeruginosa and E. coli. Reason: Seed verdict SOUND. Supporting Evidence: PMID:3018430 The P. aeruginosa DAP-decarboxylase was synthesized constitutively in P. aeruginosa as well as in E. coli |
| GO:0009085 L-lysine biosynthetic process | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN000159906, seeded by M. tuberculosis LysA. Reason: DAPDC performs the last step of the DAP pathway to lysine; complementation of lysine auxotrophs confirms the role. The more specific "via diaminopimelate" term (GO:0009089) is obsolete, so this is the right level. Supporting Evidence: PMID:3018430 This gene complemented a lys mutation at the lys-12 locus of P. aeruginosa and a lysA defect in Escherichia coli. |
| GO:0009085 L-lysine biosynthetic process | IEA GO_REF:0000120 | ACCEPT | Summary: Combined IEA for lysine biosynthesis. Reason: Consistent with the IBA row above. |
| GO:0030170 pyridoxal phosphate binding | IEA GO_REF:0000104 | KEEP AS NON CORE | Summary: UniRule PLP-cofactor term for a fold-type III decarboxylase. Reason: PLP is the cofactor of all LysA-family decarboxylases (UniProt FT MOD_RES N6-(pyridoxal phosphate)lysine); a cofactor-binding term is correct but not the core activity. |
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Download this section (compressed HTML)Q: Why is P. aeruginosa lysA expressed constitutively, without the LysR-type lysine regulation seen for E. coli lysA?
Experiment: Purify P. aeruginosa LysA and determine its kinetic parameters for meso-DAP, to confirm stereospecificity and compare with E. coli LysA.
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