id: Q9HT12
gene_symbol: parB
taxon:
  id: NCBITaxon:208964
  label: Pseudomonas aeruginosa PAO1
status: COMPLETE
locus_tag: PA5562
description: >-
  P. aeruginosa PAO1 is one of NCBI's curated reference genomes, so PGAP does
  not annotate its assembly. The identical protein WP_003100240.1 is
  annotated by PGAP in other genomes (39,835 assemblies in its
  identical-protein record, 2026-10-24). As for the P. putida ortholog, it is
  named "ParB/RepB/Spo0J family partition protein" from SPARCLE architecture
  11445026 and carries only DNA binding. TIGR00180, which also matches and
  carries chromosome segregation, does not pass on its GO because it does
  not name the protein. The prediction is less precise than the
  experimentally supported sequence-specific DNA binding. The directly
  measured parS-stimulated CTPase activity of this protein (PMID:38843873)
  has no source in PGAP.
references:
- id: PMID:33270901
  title: 'RefSeq: expanding the Prokaryotic Genome Annotation Pipeline reach with protein family model curation.'
- id: PMID:15466051
  title: 'ParB of Pseudomonas aeruginosa: interactions with its partner ParA and its target parS and
    specific effects on bacterial growth.'
- id: PMID:25794281
  title: A single parS sequence from the cluster of four sites closest to oriC is necessary and sufficient
    for proper chromosome segregation in Pseudomonas aeruginosa.
- id: file:PSEAE/parB/parB-pgap-prediction-evidence/WP_003100240.1.gp
  title: RefSeq GenPept record WP_003100240.1
- id: file:PSEAE/parB/parB-pgap-prediction-evidence/pgap_models.yaml
  title: PGAP naming model and matched NCBI HMMs for parB
source_documents:
- genes/PSEAE/parB/parB-pgap-prediction-evidence/WP_003100240.1.gp
- genes/PSEAE/parB/parB-pgap-prediction-evidence/pgap_models.yaml
predictions:
- source_method: NCBI PGAP
  source_version: WP_003100240.1 record (modified 2025-01-01); GO from WP_ record
  source_reference_id: PMID:33270901
  predicted_term:
    id: GO:0003677
    label: DNA binding
  predicted_term_type: GO_MF
  review:
    assessment: LSP
    confidence_score: 2
    summary: >-
      True but less precise. Also in GOA (combined IEA), where the main review
      MODIFIES it to sequence-specific DNA binding (GO:0043565), supported by
      direct binding of purified ParB to parS and by binding to all ten
      genomic parS sites.
    supported_by:
    - reference_id: PMID:15466051
      supporting_text: Purified ParB binds specifically to DNA containing a putative parS sequence
    - reference_id: PMID:25794281
      supporting_text: It was found that ParB binds specifically to all ten parS sites, although with
        different affinities.