PmrB (PA4777) is the sensor histidine kinase of the PmrA-PmrB two-component system of Pseudomonas aeruginosa. It is an inner-membrane protein with two transmembrane helices around a periplasmic sensor region, a HAMP linker, a HisKA dimerisation/phosphoacceptor domain carrying the autophosphorylated His249 and an HATPase catalytic domain; it has no receiver or HPt domain. PmrA-PmrB responds to limiting Mg2+ and to cationic antimicrobial peptides, autoregulates the pmrAB operon and contributes to resistance to polymyxins and cationic peptides, in part through LPS modification genes. Activating pmrB mutations are a common cause of clinical colistin resistance.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0000155 phosphorelay sensor kinase activity | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro HisKA mapping. Reason: HisKA domain with autophosphorylation histidine and HATPase domain; core function by family. Supporting Evidence: file:PSEAE/pmrB/pmrB-uniprot.txt PF00512; HisKA file:PSEAE/pmrB/pmrB-uniprot.txt Phosphohistidine; by autocatalysis |
| GO:0000160 phosphorelay signal transduction system | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN001976770, seeded by E. coli PhoQ (P23837), O69729 and this gene. Reason: True by architecture and partner genetics; the IBD stands on the other seeds. Supporting Evidence: PMID:14507375 We have identified a second two-component regulatory system, PmrA-PmrB, that regulates resistance to polymyxin B and cationic antimicrobial peptides. file:PSEAE/pmrB/pmrB-uniprot.txt PF00512; HisKA |
| GO:0000160 phosphorelay signal transduction system | IDA PMID:14507375 Cationic antimicrobial peptides activate a two-component reg... | ACCEPT | Summary: PseudoCAP IDA seed of PTN001976770. The abstract reports mutant and reporter-fusion data only. Reason: The claim is true of PmrB and is accepted with the IBA and IEA rows. As a seed it is UNVERIFIABLE: the full text was not read; the evidence in the abstract is genetic (IMP-type). Recorded as a SUSPECTED evidence-code defect, as for pfeS. Supporting Evidence: PMID:14507375 We have identified a second two-component regulatory system, PmrA-PmrB, that regulates resistance to polymyxin B and cationic antimicrobial peptides. PMID:14507375 Inactivation of the pmrB sensor kinase and pmrA response regulator greatly decreased the expression of the operon encoding pmrA-pmrB |
| GO:0000160 phosphorelay signal transduction system | IEA GO_REF:0000002 | ACCEPT | Summary: InterPro mapping. Reason: As for the IBA row. Supporting Evidence: file:PSEAE/pmrB/pmrB-uniprot.txt PF00512; HisKA |
| GO:0004673 protein histidine kinase activity | IEA GO_REF:0000003 | TOO GENERAL | Summary: EC 2.7.13.3 mapping. Reason: Entailed by GO:0000155 phosphorelay sensor kinase activity, accepted here. |
| GO:0004673 protein histidine kinase activity | ISS GO_REF:0000024 | TOO GENERAL | Summary: ISS from Salmonella PmrB (P40719). Reason: Entailed by GO:0000155, accepted here. |
| GO:0005886 plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN001976770, seeded by PhoQ, P77485 and P30844. Reason: Two predicted TM helices place PmrB in the inner membrane. Supporting Evidence: file:PSEAE/pmrB/pmrB-uniprot.txt Multi-pass membrane |
| GO:0007165 signal transduction | IEA GO_REF:0000002 | TOO GENERAL | Summary: InterPro mapping. Reason: Entailed by GO:0000160 phosphorelay signal transduction system, accepted here. |
| GO:0016020 membrane | IEA GO_REF:0000120 | TOO GENERAL | Summary: Membrane mapping. Reason: Entailed by GO:0005886 plasma membrane, accepted here. |
| GO:0016772 transferase activity, transferring phosphorus-containing groups | IEA GO_REF:0000002 | TOO GENERAL | Summary: InterPro mapping. Reason: Entailed by GO:0000155. |
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Download this section (compressed HTML)Experiment: Test autophosphorylation of the purified PmrB cytoplasmic domain and phosphotransfer to PmrA, and whether cationic peptides bind the periplasmic region directly.
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