PutP (PA0783) is the sodium/proline symporter of Pseudomonas aeruginosa, a multi-pass inner-membrane transporter of the solute:sodium symporter family (TC 2.A.21) that couples L-proline uptake to the inward sodium gradient. It is 77-84% identical to the characterised PutP proteins of Escherichia coli and other pseudomonads. putP forms an operon with putA, which encodes the bifunctional proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase, and the operon is induced by proline through the AraC-family activator PruR. A putP mutant grows poorly on proline as sole carbon and nitrogen source, so PutP is the major proline uptake system for proline catabolism.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0005298 proline:sodium symporter activity | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN001371338, seeded by E. coli PutP (P07117) and Salmonella PutP (P10502), both with direct sodium/proline symport data. Reason: PutP of PAO1 is 77% identical to E. coli PutP and sits in the same subfamily (NCBIfam TIGR02121 Na_Pro_sym). Sodium-coupled proline symport is the core activity. Supporting Evidence: PMID:12270821 PutP PAO is highly homologous to the PutP proteins of P. fluorescens (84% identity), P. putida (80% identity), and E. coli (77% identity). file:PSEAE/putP/putP-uniprot.txt Catalyzes the sodium-dependent uptake of extracellular L- proline. |
| GO:0005298 proline:sodium symporter activity | IEA GO_REF:0000120 | ACCEPT | Summary: Combined IEA (UniRule/InterPro Na/Pro symporter). Reason: Consistent with the IBA row; core activity. |
| GO:0005886 plasma membrane | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN005155964, seeded by E. coli PutP, PanF and ActP (IDA). Reason: Multi-pass inner-membrane symporter; plasma membrane is correct. |
| GO:0005886 plasma membrane | IEA GO_REF:0000120 | ACCEPT | Summary: UniRule subcellular location, cell inner membrane. Reason: Consistent with the IBA row. |
| GO:0006814 sodium ion transport | IEA GO_REF:0000002 | MODIFY | Summary: InterPro2GO from the Na/Pro symporter signature. Reason: Correct but general. As a sodium-coupled symporter PutP moves Na+ into the cell with proline, so the specific term is sodium ion import across plasma membrane. Proposed replacements: sodium ion import across plasma membrane |
| GO:0015193 L-proline transmembrane transporter activity | IBA GO_REF:0000033 | ACCEPT | Summary: IBA from PTN001371338, seeded by B. subtilis PutP (P94392, CACAO IMP) and this gene's own PseudoCAP IMP (expected self-appearance). Reason: Correct; L-proline is the transported substrate. Supporting Evidence: PMID:12270821 Thus, PA0781 appears to be irrelevant to proline utilization and PutP PAO is the major proline transporter for proline utilization in strain PAO1, as it is in enteric bacteria |
| GO:0015193 L-proline transmembrane transporter activity | IMP PMID:12270821 Divergent structure and regulatory mechanism of proline cata... | ACCEPT | Summary: PseudoCAP seed row for PTN001371338 and PTN005155964. Full text: the putP knockout grows poorly on proline as sole carbon and nitrogen source; no uptake assay. Seed verdict SOUND. Reason: A growth phenotype rather than a transport assay, but a specific one, and the authors' conclusion agrees with the 77% identity to E. coli PutP. Supporting Evidence: PMID:12270821 Thus, PA0781 appears to be irrelevant to proline utilization and PutP PAO is the major proline transporter for proline utilization in strain PAO1, as it is in enteric bacteria |
| GO:0015824 proline transport | IBA GO_REF:0000033 | MODIFY | Summary: IBA from PTN001371338, seeded by E. coli and Salmonella PutP. Reason: Correct but general. PutP imports L-proline across the inner membrane; GO:1904271 L-proline import across plasma membrane is_a proline transport. Propagation Review Root cause: NO FAILURE CORE Failure modes: GRANULARITY MISMATCH Sources checked: PANTHER:PTN001371338 · PTN001371338 SUPPORTS TRANSFER PutP subfamily node; the transfer is sound and proline transport is a fair node-level term. The gene-level term is the import descendant. UniProtKB:P07117 · E. coli PutP SUPPORTS TRANSFER Experimental proline transport and Na+/proline symport. UniProtKB:P10502 · Salmonella PutP SUPPORTS TRANSFER Experimental proline transport. Proposed replacements: L-proline import across plasma membrane Supporting Evidence: file:PSEAE/putP/putP-uniprot.txt Catalyzes the sodium-dependent uptake of extracellular L- proline. |
| GO:0015824 proline transport | IEA GO_REF:0000120 | MODIFY | Summary: Combined IEA proline transport. Reason: Correct but general; same refinement as the IBA row. Proposed replacements: L-proline import across plasma membrane |
| GO:0016020 membrane | IEA GO_REF:0000002 | TOO GENERAL | Summary: InterPro2GO membrane. Reason: Entailed by the accepted GO:0005886 plasma membrane (is_a GO:0016020). |
| GO:0022857 transmembrane transporter activity | IEA GO_REF:0000002 | TOO GENERAL | Summary: InterPro2GO from the sodium:solute symporter family signature. Reason: Entailed by the accepted GO:0005298 and GO:0015193 (both is_a GO:0022857). |
| GO:0031402 sodium ion binding | IEA GO_REF:0000120 | KEEP AS NON CORE | Summary: UniRule sodium ion binding. Reason: Sodium-coupled symporters bind Na+ as co-substrate, so the claim is true, but it is part of the symport mechanism rather than a separate function. |
| GO:0035524 proline transmembrane transport | IEA GO_REF:0000108 | TOO GENERAL | Summary: Inter-ontology inference from GO:0015193. Reason: Entailed by GO:1904271 L-proline import across plasma membrane, asserted in this review. |
| GO:0055085 transmembrane transport | IBA GO_REF:0000033 | TOO GENERAL | Summary: IBA from PTN005155964, seeded by E. coli PanF and ActP. Reason: Sound at this broad SSS-family node, but entailed by GO:1904271 asserted in this review. |
| GO:0055085 transmembrane transport | IEA GO_REF:0000002 | TOO GENERAL | Summary: InterPro2GO transmembrane transport. Reason: Entailed by GO:1904271 asserted in this review. |
| GO:1905039 carboxylic acid transmembrane transport | IEA GO_REF:0000108 | TOO GENERAL | Summary: Inter-ontology inference from GO:0005298. Reason: Entailed by GO:1904271 asserted in this review. |
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