strB encodes an aminoglycoside phosphotransferase in Pseudomonas aeruginosa. UniProt/CARD identify this entry as an aminoglycoside-modifying resistance enzyme that inactivates aminoglycosides by phosphorylation. UniProt names the protein 'Streptomycin resistance protein B' (accession O84956).
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0016773 phosphotransferase activity, alcohol group as acceptor | IEA GO_REF:0000002 | MODIFY | Summary: The existing MF term is directionally correct but less informative than the family-specific AMR activity aminoglycoside phosphotransferase activity. Reason: Replace the broad or over-specific electronic term 'phosphotransferase activity, alcohol group as acceptor' with aminoglycoside phosphotransferase activity based on UniProt/CARD determinant identity and the curated ARO->GO mapping. Proposed replacements: aminoglycoside phosphotransferase activity Supporting Evidence: file:genes/PSEAI/strB/strB-uniprot.txt DR CARD; ARO:3002660; APH(6)-Id; ARO:0001004; antibiotic inactivation. |
| GO:0019748 secondary metabolic process | IEA GO_REF:0000002 | MODIFY | Summary: This is a broad process term and does not capture the AMR role of the enzyme. Reason: The more informative process is response to antibiotic; the molecular activity should be captured by aminoglycoside phosphotransferase activity. Proposed replacements: response to antibiotic Supporting Evidence: file:genes/PSEAI/strB/strB-uniprot.txt DR CARD; ARO:3002660; APH(6)-Id; ARO:0001004; antibiotic inactivation. |
| GO:0034071 aminoglycoside phosphotransferase activity | RCA file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml | NEW | Summary: NEW candidate annotation from the curated ARO->GO mapping: aminoglycoside phosphotransferase activity. Reason: The UniProt record cross-references a CARD/ARO AMR determinant, and the curated ARO->GO mapping projects the family-specific molecular function aminoglycoside phosphotransferase activity. This focused draft accepts the candidate as a curator lead for this AMR determinant. Supporting Evidence: file:projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv O84956 ARO:3002660 ARO:3000114 narrower RO:0002327 GO:0034071 aminoglycoside phosphotransferase activity file:genes/PSEAI/strB/strB-uniprot.txt DR CARD; ARO:3002660; APH(6)-Id; ARO:0001004; antibiotic inactivation. |
| GO:0046677 response to antibiotic | RCA file:projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml | NEW | Summary: NEW process annotation: strB is an AMR determinant involved in antibiotic response/resistance. Reason: CARD/ARO identity places this gene in an antimicrobial-resistance determinant family; response to antibiotic is the appropriate high-level GO biological process for the resistance role. Supporting Evidence: file:genes/PSEAI/strB/strB-uniprot.txt DR CARD; ARO:3002660; APH(6)-Id; ARO:0001004; antibiotic inactivation. |
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Download this section (compressed HTML)Q: Is the ARO-derived aminoglycoside phosphotransferase activity annotation sufficiently specific for strB, or is a narrower substrate/site-specific GO term warranted?
Experiment: Biochemically assay purified strB against representative antibiotic substrates for the inferred AMR family and measure loss of drug activity or target modification.
Type: in vitro enzyme assay
Focused AMR batch review. UniProt accession: O84956. Source organism: Pseudomonas aeruginosa.
DR CARD; ARO:3002660; APH(6)-Id; ARO:0001004; antibiotic inactivation.projects/ANTIMICROBIAL_RESISTANCE/data/candidate_new_annotations.tsv and projects/ANTIMICROBIAL_RESISTANCE/aro2go.sssom.yaml.Loading supporting content…
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