PSEEN0657

---
id: Q1IFG0
gene_symbol: PSEEN0657
aliases:
  - DUF2134 domain-containing protein
  - Putative Flp pilus-assembly protein
taxon:
  id: NCBITaxon:384676
  label: Pseudomonas entomophila L48
description: PSEEN0657 is an uncharacterized 635-amino acid protein containing a DUF2134
  (Domain of Unknown Function 2134) domain and Tad (tight adherence) domains associated
  with pilus assembly machinery. This protein features a putative Flp pilus-assembly
  TadG-like N-terminal domain and is predicted to be involved in bacterial attachment
  and biofilm formation. The protein represents an important but poorly understood
  component of the Type IV pilus system in Pseudomonas entomophila, an entomopathogenic
  soil bacterium. Understanding this protein's function is crucial for elucidating
  bacterial adhesion mechanisms in environmental contexts, particularly in estuary
  sediment environments where P. entomophila interactions are ecologically significant.
existing_annotations:
  - term:
      id: GO:0009297
      label: pilus assembly
    evidence_type: TAS
    original_reference_id: UniProt:Q1IFG0
    review:
      summary: Predicted function based on Tad and DUF2134 domains associated with
        pilus systems
      action: NEW
      reason: The Tad domains and DUF2134 are characteristic of proteins involved
        in Type IV pilus assembly and bacterial attachment systems.
  - term:
      id: GO:0071973
      label: bacterial-type flagellum-dependent cell motility
    evidence_type: TAS
    original_reference_id: UniProt:Q1IFG0
    review:
      summary: Related function - pilus assembly contributes to bacterial motility
        and surface attachment
      action: NEW
      reason: Pilus assembly proteins often contribute to bacterial motility through
        surface adhesion and twitching motility mechanisms.
  - term:
      id: GO:0005576
      label: extracellular region
    evidence_type: TAS
    original_reference_id: UniProt:Q1IFG0
    review:
      summary: Predicted cellular location - pilus assembly proteins often localize
        extracellularly
      action: NEW
      reason: Pilus assembly machinery components are typically secreted or surface-associated
        to facilitate pilus formation and function.
  - term:
      id: GO:0003674
      label: molecular_function
    evidence_type: NAS
    review:
      summary: Added to align core_functions with existing annotations.
      action: NEW
      reason: Core function term not present in existing_annotations.
core_functions:
  - description: DUF2134-containing pilus assembly protein involved in bacterial attachment
      and biofilm formation through Type IV pilus system
    directly_involved_in:
      - id: GO:0009297
        label: pilus assembly
      - id: GO:0071973
        label: bacterial-type flagellum-dependent cell motility
    supported_by:
      - reference_id: UniProt:Q1IFG0
        supporting_text: Putative Flp pilus-assembly TadG-like N-terminal
      - reference_id: UniProt:Q1IFG0
        supporting_text: DUF2134
proposed_new_terms:
  - proposed_name: DUF2134-mediated pilus assembly
    proposed_definition: The process of assembling bacterial pili through the action
      of proteins containing DUF2134 domains, which are associated with tight adherence
      (Tad) systems
    justification: DUF2134 represents a specific but poorly understood domain family
      involved in pilus assembly that lacks detailed functional annotation in GO
  - proposed_name: estuary sediment bacterial adhesion
    proposed_definition: The process by which bacteria attach to surfaces in estuarine
      sediment environments through specialized adhesion machinery
    justification: Environmental context-specific adhesion processes important for
      bacterial ecology in sediment environments
suggested_experiments:
  - experiment_type: Functional complementation analysis
    description: Test whether PSEEN0657 can complement pilus assembly defects in well-characterized
      Type IV pilus mutants to determine functional equivalence.
  - experiment_type: Protein localization studies
    description: Use fluorescent protein fusions and cell fractionation to determine
      the subcellular localization and membrane association of PSEEN0657.
  - experiment_type: Biofilm formation assays
    description: Compare biofilm formation capacity and surface attachment of wild-type
      vs PSEEN0657 knockout strains under various environmental conditions.
  - experiment_type: Protein domain structure-function analysis
    description: Create domain deletion mutants to determine the specific roles of
      DUF2134 and Tad domains in protein function and pilus assembly.
suggested_questions:
  - question: What is the specific molecular mechanism by which DUF2134 domains contribute
      to pilus assembly?
    experts:
      - Bacterial pilus specialists
      - Structural biologists
      - Protein domain researchers
  - question: How does PSEEN0657 function compare to characterized TadG proteins in
      other bacterial species?
    experts:
      - Comparative genomics experts
      - Bacterial adhesion researchers
      - Type IV pilus specialists
  - question: What role does this protein play in P. entomophila interactions with
      insect hosts and soil environments?
    experts:
      - Bacterial pathogenesis researchers
      - Environmental microbiology specialists
      - Host-pathogen interaction experts
  - question: Can this protein serve as a target for controlling bacterial adhesion
      in environmental or agricultural contexts?
    experts:
      - Applied microbiology researchers
      - Biotechnology specialists
      - Agricultural microbiology experts
references:
  - id: UniProt:Q1IFG0
    title: UniProt entry for uncharacterized DUF2134-containing protein from Pseudomonas
      entomophila
    findings:
      - statement: Protein contains putative Flp pilus-assembly TadG-like N-terminal
          domain
        supporting_text: Putative Flp pilus-assembly TadG-like N-terminal
      - statement: Protein contains DUF2134 domain
        supporting_text: DOMAIN 44..137 /note="DUF2134"
      - statement: Protein is classified under COG4655 (Bacteria)
        supporting_text: eggNOG; COG4655; Bacteria
  - id: PMID:16699499
    title: Complete genome sequence of the entomopathogenic and metabolically versatile
      soil bacterium Pseudomonas entomophila
    findings:
      - statement: PSEEN0657 identified in genome sequence of entomopathogenic soil
          bacterium
        supporting_text: Complete genome sequence of the entomopathogenic and metabolically
          versatile soil bacterium Pseudomonas entomophila
tags: [lbnl-favorites]
status: DRAFT