PP_0075

UniProt ID: Q88RQ4
Organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Review Status: DRAFT
📝 Provide Detailed Feedback

Gene Description

PP_0075 is a multi-pass SLC26A/SulP-family membrane transporter with a cytoplasmic STAS domain. It lies next to the choline-sulfatase gene betC, but its transported substrate, coupling mechanism, and proposed role in choline-O-sulfate uptake have not been established experimentally.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0016020 membrane
IEA
GO_REF:0000120 		KEEP AS NON CORE
Summary: The multi-pass architecture strongly supports membrane localization.
Reason: Membrane location is valid topology but is ancillary to the unresolved transport activity.
Supporting Evidence:
file:PSEPK/PP_0075/PP_0075-uniprot.txt
SUBCELLULAR LOCATION: Membrane
GO:0055085 transmembrane transport
IEA
GO_REF:0000002 		ACCEPT
Summary: Accept generic transmembrane transport without assigning a specific cargo.
Reason: Nine predicted membrane-spanning segments and the SLC26A/SulP family assignment support a transport role. Neither the submitted product name nor genomic adjacency establishes choline-O-sulfate specificity.
Supporting Evidence:
file:PSEPK/PP_0075/PP_0075-uniprot.txt
InterPro; IPR001902; SLC26A/SulP_fam.
file:PSEPK/PP_0075/PP_0075-deep-research-openscientist.md
PP_0075 encodes an integral inner-membrane secondary transporter belonging to the **SulP / SLC26 anion-permease superfamily**.
GO:0022857 transmembrane transporter activity
IEA
file:PSEPK/PP_0075/PP_0075-uniprot.txt 		NEW
Summary: Add the generic molecular activity implied by the multi-pass SLC26A/SulP architecture.
Reason: The fetched GOA set contains the transport process but no molecular function. A generic transporter activity is defensible while exact cargo specificity remains unresolved.
Supporting Evidence:
file:PSEPK/PP_0075/PP_0075-uniprot.txt
InterPro; IPR001902; SLC26A/SulP_fam.

Core Functions

Multi-pass SLC26A/SulP-family transmembrane transporter of unresolved physiological cargo and coupling mechanism.

Molecular Function:
transmembrane transporter activity
Directly Involved In:
transmembrane transport
Supporting Evidence:
  • file:PSEPK/PP_0075/PP_0075-uniprot.txt
    InterPro; IPR001902; SLC26A/SulP_fam.
  • file:PSEPK/PP_0075/PP_0075-deep-research-openscientist.md
    No direct transport assay exists for PP_0075.

References

Loading supporting content…

Download this section (compressed HTML)

Suggested Questions for Experts

Q: What solute does PP_0075 transport, and does it functionally couple to the adjacent PP_0076 binding protein?

Suggested Experiments

Experiment: Measure uptake of choline-O-sulfate, sulfate, choline, and related solutes in clean PP_0075 deletion and complementation strains, then reconstitute transport in proteoliposomes.

Deep Research

OpenScientist

(PP_0075-deep-research-openscientist.md)

Loading supporting content…

Download this section (compressed HTML)

📚 Additional Documentation

Notes

(PP_0075-notes.md)

Loading supporting content…

Download this section (compressed HTML)

📄 View Raw YAML

Loading supporting content…

Download this section (compressed HTML)