PP_2483 encodes a MobA-like NTP-transferase-domain protein. KEGG assigns it to MocA orthology K07141, but the broad PANTHER family does not distinguish MobA from MocA-like activities and local pairwise comparisons place Q88K10 closer to Escherichia coli MobA than MocA. Its physiological substrate, nucleotide specificity, and pathway role therefore remain unresolved.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0016779 nucleotidyltransferase activity | IEA GO_REF:0000117 | ACCEPT | Summary: The broad nucleotidyltransferase prediction is plausible, but the physiological reaction and nucleotide specificity are unresolved. Reason: The MobA-like domain and KEGG K07141 assignment support an NTP-transferase family placement, but PTHR43777:SF1 is not MocA-specific and the saved sequence comparison places Q88K10 closer to E. coli MobA than MocA. Neither CTP-dependent MocA activity nor GTP-dependent MobA activity has been demonstrated for Q88K10. Supporting Evidence: file:PSEPK/moaA/moaA-bioinformatics/mob_like_kegg_orthology.tsv PP_2483 Q88K10 ppu:PP_2483 ko:K07141 file:PSEPK/moaA/moaA-bioinformatics/RESULTS.md - PP_2483 is more similar to the E. coli MobA control (39.29%) than to the E. coli MocA control (28.49%), whereas PP_4230 is more similar to MocA (39.62%) than to MobA (28.74%). PP_2483 and PP_4230 are 48.13% identical to one another. - The generated KEGG link table maps both PP_2483 and PP_4230 to K07141. It maps the reviewed E. coli controls to distinct MobA (K03752) and MocA (K07141) orthologies, respectively. file:PSEPK/PP_2483/PP_2483-deep-research-openscientist.md **No direct experimental data** exist for PP_2483; the annotation is inferred from orthology, conserved domains, genomic context, and characterized homologs. Q88K10 is an unreviewed TrEMBL entry. |
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Download this section (compressed HTML)Q: Is PP_2483 CTP- or GTP-specific in vitro, and does it act on the molybdenum cofactor used by the neighboring molybdoenzyme system?
Experiment: Compare CTP- and GTP-dependent nucleotide transfer from purified Q88K10 to Mo-MPT and test how PP_2483 deletion affects the neighboring molybdoenzyme system.
Type: substrate-specificity assay and targeted genetics
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