PP_2638

UniProt ID: Q88JL1
Organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Review Status: DRAFT
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Gene Description

PP_2638 encodes a large BcsC-like cellulose synthase operon C protein assigned to the outer membrane and required for maximal bacterial cellulose synthesis.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0019867 outer membrane
IEA
GO_REF:0000002 		ACCEPT
Summary: Outer membrane is the appropriate location for the BcsC-like PP_2638 cellulose synthase operon C protein.
Reason: GOA assigns the Gram-negative outer membrane term and UniProt describes PP_2638 as a cellulose synthase operon C protein, consistent with an export/support component.
Supporting Evidence:
file:PSEPK/PP_2638/PP_2638-goa.tsv
GO:0019867 outer membrane
file:PSEPK/PP_2638/PP_2638-uniprot.txt
SubName: Full=Cellulose synthase operon C protein
PMID:31604608
pEtN-cellulose is secreted through the Ξ²-barrel porin BcsC
GO:0030244 cellulose biosynthetic process
IEA
GO_REF:0000002 		ACCEPT
Summary: Cellulose biosynthetic process is the appropriate pathway annotation for PP_2638.
Reason: UniProt states that PP_2638 is required for maximal bacterial cellulose synthesis and places it in bacterial cellulose biosynthesis.
Supporting Evidence:
file:PSEPK/PP_2638/PP_2638-uniprot.txt
FUNCTION: Required for maximal bacterial cellulose synthesis.
file:PSEPK/PP_2638/PP_2638-uniprot.txt
PATHWAY: Glycan metabolism; bacterial cellulose biosynthesis.
file:PSEPK/PP_2638/PP_2638-goa.tsv
GO:0030244 cellulose biosynthetic process

Core Functions

Outer-membrane cellulose synthase operon C export/support role required for maximal bacterial cellulose synthesis.

Directly Involved In:
cellulose biosynthetic process
Cellular Locations:
outer membrane
Supporting Evidence:
  • file:PSEPK/PP_2638/PP_2638-uniprot.txt
    FUNCTION: Required for maximal bacterial cellulose synthesis.
  • file:PSEPK/PP_2638/PP_2638-uniprot.txt
    PATHWAY: Glycan metabolism; bacterial cellulose biosynthesis.
  • file:PSEPK/PP_2638/PP_2638-goa.tsv
    GO:0019867 outer membrane
  • PMID:31604608
    pEtN-cellulose is secreted through the Ξ²-barrel porin BcsC
  • file:PSEPK/PP_2638/PP_2638-deep-research-openscientist.md
    PP_2638 is BcsC, the outer-membrane cellulose secretion porin.

References

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Suggested Questions for Experts

Q: What is the exact molecular role of PP_2638 in KT2440 cellulose export, pore formation, or synthase-complex assembly?

Q: Should PP_2638 be curated under a broader BcsC/export-support term if GO has or needs a more specific molecular-function representation?

Suggested Experiments

Experiment: Compare cellulose production, cell-surface cellulose retention, and extracellular cellulose export in wild-type and PP_2638 deletion strains.

Type: targeted genetics and cellulose localization assay

Experiment: Determine PP_2638 localization and complex association with BcsA/BcsB using fractionation and co-immunoprecipitation or proximity tagging.

Type: localization and protein-complex assay

Deep Research

OpenScientist

(PP_2638-deep-research-openscientist.md)

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