Conserved third (gamma) subunit of the periplasmic three-subunit gluconate 2-dehydrogenase. Its precise mechanism is unknown; the Tat signal and specific Gad-subunit-3 family support a role in assembly, export, or stability rather than an independent catalytic activity.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0033717 gluconate 2-dehydrogenase (acceptor) activity | IEA GO_REF:0000003 | MODIFY | Summary: The specific IPR027056 family and homologous three-subunit architecture support Gad complex membership. Reason: Replace the GOA enables relation with contributes_to because no autonomous catalytic activity is known for the gamma subunit. Proposed replacements: gluconate 2-dehydrogenase (acceptor) activity Supporting Evidence: PMID:9352901 complete structural genes for a 68-kDa dehydrogenase subunit, a 47-kDa cytochrome c subunit, and a 24-kDa subunit of unknown function |
| GO:0042597 periplasmic space | ISS | NEW | Summary: The Tat signal and homologous exported Gad subunit support periplasmic localization. Reason: This localization is part of the conserved membrane-associated Gad architecture. Supporting Evidence: file:PSEPK/PP_3384/PP_3384-uniprot.txt DR PROSITE; PS51318; TAT; 1. |
| GO:0019595 non-phosphorylated glucose catabolic process | ISS | NEW | Summary: Complex membership places this subunit in peripheral oxidative glucose catabolism. Reason: Adds the pathway process while leaving its unknown mechanism explicit. Supporting Evidence: PMID:9352901 The positive clone converted D-gluconate to 2-keto-D-gluconate (2KDG) in the culture medium. |
| GO:0019521 D-gluconate metabolic process | ISS | NEW | Summary: D-gluconate is the direct substrate oxidized by the Gad complex to which this gamma subunit contributes. Reason: This complements the broader non-phosphorylated glucose-catabolism term with the immediate substrate process. Supporting Evidence: PMID:9352901 The positive clone converted D-gluconate to 2-keto-D-gluconate (2KDG) in the culture medium. |
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Download this section (compressed HTML)The requested OpenScientist run finished without leaving a final gene report, so this
manual note records the evidence used for curation. Q88HH4 belongs to the specific
Gad-subunit-3 family IPR027056 and has a Tat signal. The homologous experimentally
cloned complex contains a third "24-kDa subunit of unknown function" PMID:9352901.
The protein is therefore curated as a noncatalytic or mechanistically unresolved
complex contributor, not as an independently active gluconate dehydrogenase.
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