PP_4230

Gene Description

PP_4230 encodes a MobA-like NTP-transferase-domain protein. KEGG and local sequence similarity favor a MocA-like assignment, but the broad PANTHER family does not distinguish nucleotide specificity and no Q88F68 enzyme assay establishes CTP-dependent activity. Its physiological reaction and pathway role therefore remain unresolved.

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0016779 nucleotidyltransferase activity	IEA GO_REF:0000117	ACCEPT	Summary: The broad nucleotidyltransferase prediction is plausible, but the physiological reaction and nucleotide specificity are unresolved. Reason: KEGG K07141 and modest similarity to E. coli MocA favor a MocA-like interpretation, but PTHR43777:SF1 is not MocA-specific and no target assay establishes CTP-dependent cytidylyltransferase activity for Q88F68. Supporting Evidence: file:PSEPK/moaA/moaA-bioinformatics/mob_like_kegg_orthology.tsv PP_4230 Q88F68 ppu:PP_4230 ko:K07141 file:PSEPK/moaA/moaA-bioinformatics/RESULTS.md - PP_2483 is more similar to the E. coli MobA control (39.29%) than to the E. coli MocA control (28.49%), whereas PP_4230 is more similar to MocA (39.62%) than to MobA (28.74%). PP_2483 and PP_4230 are 48.13% identical to one another. - The generated KEGG link table maps both PP_2483 and PP_4230 to K07141. It maps the reviewed E. coli controls to distinct MobA (K03752) and MocA (K07141) orthologies, respectively. file:PSEPK/PP_4230/PP_4230-deep-research-openscientist.md 1. No PP_4230-specific experimental study exists. The entire annotation is inferential. There is no published enzyme assay, knockout, structure, or complementation experiment for PP_4230 itself.

Core Functions

MobA-like nucleotidyltransferase-family protein whose physiological nucleotide substrate and pathway role remain unresolved.

Supporting Evidence:

file:PSEPK/moaA/moaA-bioinformatics/RESULTS.md
- PP_2483 is more similar to the E. coli MobA control (39.29%) than to the E. coli MocA control (28.49%), whereas PP_4230 is more similar to MocA (39.62%) than to MobA (28.74%). PP_2483 and PP_4230 are 48.13% identical to one another. - The generated KEGG link table maps both PP_2483 and PP_4230 to K07141. It maps the reviewed E. coli controls to distinct MobA (K03752) and MocA (K07141) orthologies, respectively.

References

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Suggested Experiments

Experiment: Compare CTP- and GTP-dependent nucleotide transfer from purified Q88F68 to Mo-MPT and test how PP_4230 deletion affects the neighboring molybdoenzyme system.

Type: substrate-specificity assay and targeted genetics

Gene Description

Existing Annotations Review

Core Functions

MobA-like nucleotidyltransferase-family protein whose physiological nucleotide substrate and pathway role remain unresolved.

References

Suggested Questions for Experts

Suggested Experiments

Deep Research

OpenScientist

📚 Additional Documentation

Notes

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