aacs

UniProt ID: Q88IC8
Organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Review Status: DRAFT
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Gene Description

Aacs is a predicted acetoacetate-CoA ligase that activates acetoacetate to acetoacetyl-CoA using ATP and coenzyme A. In KT2440 it is encoded near hbdH and bhbP, supporting a bacterial D-3-hydroxybutyrate utilization route that may use direct ATP-dependent acetoacetate activation; the physiological route and flux contribution of Q88IC8 have not been demonstrated directly.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0006629 lipid metabolic process
IEA
GO_REF:0000002 		MARK AS OVER ANNOTATED
Summary: This broad InterPro-derived process overstates what is known for the KT2440 protein.
Reason: The mapping imports a broad lipid-process context from InterPro. The predicted ligase chemistry does not by itself establish that Q88IC8 participates in lipid metabolism in KT2440; evidence from mammalian AACS is not transferable to this bacterial candidate.
GO:0030729 acetoacetate-CoA ligase activity
IEA
GO_REF:0000120 		ACCEPT
Summary: This is the defining EC- and family-supported Aacs reaction.
Reason: UniProt assigns EC 6.2.1.16 and both InterPro IPR005914 and PTHR42921:SF1 identify acetoacetyl-CoA synthetase.
Supporting Evidence:
file:PSEPK/aacs/aacs-uniprot.txt
Acetoacetyl-coenzyme A synthetase

Core Functions

Predicted to activate acetoacetate to acetoacetyl-CoA using ATP and coenzyme A.

Molecular Function:
acetoacetate-CoA ligase activity
Supporting Evidence:
  • file:PSEPK/aacs/aacs-uniprot.txt
    Acetoacetyl-coenzyme A synthetase
  • PMID:7140777
    The enzyme absolutely required ATP, CoA, a monovalent cation (K+, Rb+, Cs+ or NH+4) and a divalent cation (Mg2+, Mn2+, Ca2+ or Ni2+) for the activation of acetoacetate, yielding acetoacetyl-CoA, AMP and pyrophosphate in equimolar amounts.

References

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Suggested Questions for Experts

Q: Is Aacs the principal acetoacetate-activation route during KT2440 growth on D-3-hydroxybutyrate?

Suggested Experiments

Experiment: Compare acetoacetate-CoA ligase activity, acetoacetyl-CoA abundance, and growth on D-3-hydroxybutyrate in wild type, aacs deletion, and complemented KT2440 strains.

Type: targeted metabolomics and carbon-source growth assay

📚 Additional Documentation

Notes

(aacs-notes.md)

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