ID   Q88IC8_PSEPK            Unreviewed;       650 AA.
AC   Q88IC8;
DT   01-JUN-2003, integrated into UniProtKB/TrEMBL.
DT   01-JUN-2003, sequence version 1.
DT   28-JAN-2026, entry version 110.
DE   SubName: Full=Acetoacetyl-coenzyme A synthetase {ECO:0000313|EMBL:AAN68679.1};
DE            EC=6.2.1.16 {ECO:0000313|EMBL:AAN68679.1};
GN   Name=aacs {ECO:0000313|EMBL:AAN68679.1};
GN   OrderedLocusNames=PP_3071 {ECO:0000313|EMBL:AAN68679.1};
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Pseudomonadales; Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488 {ECO:0000313|EMBL:AAN68679.1, ECO:0000313|Proteomes:UP000000556};
RN   [1] {ECO:0000313|EMBL:AAN68679.1, ECO:0000313|Proteomes:UP000000556}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440
RC   {ECO:0000313|Proteomes:UP000000556};
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L., Beanan M., DeBoy R.T., Daugherty S., Kolonay J., Madupu R.,
RA   Nelson W., White O., Peterson J., Khouri H., Hance I., Chris Lee P.,
RA   Holtzapple E., Scanlan D., Tran K., Moazzez A., Utterback T., Rizzo M.,
RA   Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D.,
RA   Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N.,
RA   Dusterhoft A., Tummler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
RN   [2] {ECO:0000313|EMBL:AAN68679.1, ECO:0000313|Proteomes:UP000000556}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440
RC   {ECO:0000313|Proteomes:UP000000556};
RX   PubMed=26913973; DOI=10.1111/1462-2920.13230;
RA   Belda E., van Heck R.G., Lopez-Sanchez M.J., Cruveiller S., Barbe V.,
RA   Fraser C., Klenk H.P., Petersen J., Morgat A., Nikel P.I., Vallenet D.,
RA   Rouy Z., Sekowska A., Martins Dos Santos V.A., de Lorenzo V., Danchin A.,
RA   Medigue C.;
RT   "The revisited genome of Pseudomonas putida KT2440 enlightens its value as
RT   a robust metabolic chassis.";
RL   Environ. Microbiol. 18:3403-3424(2016).
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000256|ARBA:ARBA00006432}.
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DR   EMBL; AE015451; AAN68679.1; -; Genomic_DNA.
DR   RefSeq; WP_010953970.1; NC_002947.4.
DR   AlphaFoldDB; Q88IC8; -.
DR   STRING; 160488.PP_3071; -.
DR   PaxDb; 160488-PP_3071; -.
DR   KEGG; ppu:PP_3071; -.
DR   PATRIC; fig|160488.4.peg.3243; -.
DR   eggNOG; COG0365; Bacteria.
DR   HOGENOM; CLU_000022_3_3_6; -.
DR   OrthoDB; 9803968at2; -.
DR   PhylomeDB; Q88IC8; -.
DR   BioCyc; PPUT160488:G1G01-3264-MONOMER; -.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0030729; F:acetoacetate-CoA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR   CDD; cd05943; AACS; 1.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR   InterPro; IPR005914; Acac_CoA_synth.
DR   InterPro; IPR032387; ACAS_N.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_synth/lig_dom.
DR   InterPro; IPR042099; ANL_N_sf.
DR   NCBIfam; TIGR01217; ac_ac_CoA_syn; 1.
DR   NCBIfam; NF002937; PRK03584.1; 1.
DR   PANTHER; PTHR42921; ACETOACETYL-COA SYNTHETASE; 1.
DR   PANTHER; PTHR42921:SF1; ACETOACETYL-COA SYNTHETASE; 1.
DR   Pfam; PF16177; ACAS_N; 1.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF13193; AMP-binding_C; 1.
DR   SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:AAN68679.1};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000556}.
FT   DOMAIN          36..91
FT                   /note="Acetyl-coenzyme A synthetase N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF16177"
FT   DOMAIN          95..469
FT                   /note="AMP-dependent synthetase/ligase"
FT                   /evidence="ECO:0000259|Pfam:PF00501"
FT   DOMAIN          542..612
FT                   /note="AMP-binding enzyme C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF13193"
SQ   SEQUENCE   650 AA;  72303 MW;  D7EF184A106211D3 CRC64;
     MNDVLWRPST TQIEASRMDA FRRRVNLRYN LQLNDYQALH GWSIEQRPAF WQTLAEYFQV
     HWHTPPSQVL SEGPQMPDAQ WFANATLNFA EHLLRRRDDR PAVVAVREDG QREVFTHAEL
     AAQVAGLQTA FKAVGIVPGD RVAAIMPNTW QTLVAMLACT SLGAVWSTSS PEFGVHGIID
     RFGQIEPKLL IVCAGYQYAG KAIDQVTKVN QVCAQLPGLE HLIVVPHTRK GTRADEFQAA
     NVSLWNDFFQ PGGEPRFTPL PFDHPLYILY SSGTTGVPKC IVHRAGGVLL QHLKEHGLHN
     DLKADDVLFY YTTCGWMMWN WLASGLAVGA TLVLYDGSPF HPGPERLLDL IEAEGIHAFG
     TSAKYLAALE QAGLEPANSH CLTSLRLLLS TGSPLSPHSY DYVYDKIKAD LWLVSMSGGT
     DIVSCFVLGN PTLPVRRGEI QCKGLGMAVE VWNEQGQPVV DEKGELVCTR NFPSMPLGFW
     NDPDGRRYHD AYFSQFPGVW AQGDYAEQRA SGGMVIHGRS DAVLNPGGVR IGTAEIYRQV
     EKVEQVVESV AIGQDWNGDV RVVLFVRLQD GLQLDEALRQ HIRQVIRQYT TPRHVPAVIA
     QVSDIPRTIS GKLVELAIRD VVHGRPVKNT DALANPEALE QFRDRAELKS
//