AlgE is the predicted outer-membrane alginate export channel of Pseudomonas putida KT2440. Specific family identity and experimentally characterized Pseudomonas aeruginosa orthologs support a beta-barrel pore that provides a route for polymer secretion. A modeled KT2440 AlgEKX arrangement links it to the periplasmic AlgKβAlgX complex, but that complete assembly and native target polymer transport have not been experimentally established.
| GO Term | Evidence | Action | Reason |
|---|---|---|---|
| GO:0009279 cell outer membrane | IEA GO_REF:0000044 | ACCEPT | Summary: AlgE is an outer-membrane protein. Reason: The curated target compartment and specific channel family plus ortholog structure support localization. A predicted KT2440 AlgEKX model is not a direct localization assay. Supporting Evidence: file:PSEPK/algE/algE-deep-research-falcon.md AlgE is an **outer-membrane (OM) Ξ²-barrel porin** that forms the **outer-membrane channel for alginate export** file:PSEPK/algE/algE-uniprot.txt SUBCELLULAR LOCATION: Cell outer membrane PMID:21778407 monomeric 18-stranded Ξ²-barrel |
| GO:0042121 alginic acid biosynthetic process | IEA GO_REF:0000041 | ACCEPT | Summary: AlgE contributes the outer-membrane polymer export route. Reason: Family-supported pore function and Pa channel/loop-mutant evidence support machinery participation in alginate production; polymer synthesis is performed by Alg8, not AlgE. Supporting Evidence: file:PSEPK/algE/algE-uniprot.txt Belongs to the AlgE family. file:PSEPK/algE/algE-uniprot.txt Has non-porin-like, channel-forming properties and probably functions as an alginate permeability pore PMID:21778407 monomeric 18-stranded Ξ²-barrel |
| GO:0015159 polysaccharide transmembrane transporter activity | ISS PMID:21778407 Structural basis for alginate secretion across the bacterial... | NEW | Summary: AlgE-family identity supports inferred polysaccharide transport across the outer membrane. Reason: GO:0015159 explicitly describes transfer of a polysaccharide across a membrane and therefore includes alginate. The target curated probable alginate-pore function, specific AlgE family and Pa structure/flux/loop-mutant evidence support an ISS proposal. Neither existing row is a molecular function, and no existing or replacement MF duplicates this claim. Native KT2440 polymer-translocation measurements are not asserted. A separate alginate-channel NTR is not required to distinguish this activity from ion transport. Supporting Evidence: file:PSEPK/algE/algE-uniprot.txt Belongs to the AlgE family. file:PSEPK/algE/algE-uniprot.txt Has non-porin-like, channel-forming properties and probably functions as an alginate permeability pore PMID:21778407 monomeric 18-stranded Ξ²-barrel |
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Download this section (compressed HTML)Q: Does KT2440 AlgE form an alginate-specific outer-membrane pore with substrate selectivity comparable to characterized AlgE homologs?
Experiment: Reconstitute AlgE in proteoliposomes or planar bilayers and test alginate-dependent channel conductance and polymer passage.
Type: membrane channel assay
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