argE

Existing Annotations Review

GO Term	Evidence	Action	Reason
GO:0005737 cytoplasm	IEA GO_REF:0000044	ACCEPT	Summary: The cytoplasm is the inferred compartment of this soluble biosynthetic enzyme. Reason: This location is part of the core metabolic role. It is supported by conserved soluble-enzyme biology and ArgE-specific family assignment; the ARBA location line alone is not treated as evidence. Supporting Evidence: file:PSEPK/argE/argE-uniprot.txt DR NCBIfam; TIGR01892; AcOrn-deacetyl; 1.
GO:0006526 L-arginine biosynthetic process	IEA GO_REF:0000120	ACCEPT	Summary: The enzyme contributes catalytic chemistry to L-arginine biosynthesis. Reason: The enzyme catalyzes a chemical step of the acetylated-ornithine route to arginine, supported by curated reaction and family classification. The native insertion mutant remains prototrophic (PMID31451546), compatible with metabolic redundancy; this does not demonstrate inactivity or identify its compensator. Supporting Evidence: file:PSEPK/argE/argE-uniprot.txt DR NCBIfam; TIGR01892; AcOrn-deacetyl; 1. PMID:31451546 It should be noted that these three mutants do not display arginine auxotrophy
GO:0008777 acetylornithine deacetylase activity	IEA GO_REF:0000120	ACCEPT	Summary: This is the substrate-specific molecular function predicted for ArgE. Reason: EC 3.5.1.16, HAMAP MF_01108, NCBIfam TIGR01892, and the ArgE M20A subfamily agree. This assignment uses curated catalytic/family evidence; it is not a direct purified-enzyme measurement in KT2440. Supporting Evidence: file:PSEPK/argE/argE-uniprot.txt DR NCBIfam; TIGR01892; AcOrn-deacetyl; 1.
GO:0016787 hydrolase activity	IEA GO_REF:0000120	MODIFY	Summary: Refine the true broad annotation to the supported substrate-specific function. Reason: The broad term is biologically compatible with the enzyme; the supported specific activity/process already present in this review is more informative. Breadth alone does not make a claim over-annotated. Proposed replacements: acetylornithine deacetylase activity Supporting Evidence: file:PSEPK/argE/argE-uniprot.txt DR NCBIfam; TIGR01892; AcOrn-deacetyl; 1.
GO:0019213 deacetylase activity	IEA GO_REF:0000002	MODIFY	Summary: Refine the true broad annotation to the supported substrate-specific function. Reason: The broad term is biologically compatible with the enzyme; the supported specific activity/process already present in this review is more informative. Breadth alone does not make a claim over-annotated. Proposed replacements: acetylornithine deacetylase activity Supporting Evidence: file:PSEPK/argE/argE-uniprot.txt DR NCBIfam; TIGR01892; AcOrn-deacetyl; 1.