argE

UniProt ID: Q88CJ5
Organism: Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)
Review Status: DRAFT
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Gene Description

ArgE (PP_5186) is a predicted M20A-family acetylornithine deacetylase (EC 3.5.1.16) that hydrolyzes N-acetyl-L-ornithine to L-ornithine and acetate in the linear acetylated-ornithine route to L-arginine. Direct KT2440 enzymology is unavailable, and its condition-specific contribution relative to the cyclic ArgJ route is unresolved.

Existing Annotations Review

GO Term Evidence Action Reason
GO:0005737 cytoplasm
IEA
GO_REF:0000044 		KEEP AS NON CORE
Summary: ArgE is predicted to be a soluble cytoplasmic enzyme.
Reason: The location is plausible and electronically supported but is secondary to the reaction.
GO:0006526 L-arginine biosynthetic process
IEA
GO_REF:0000120 		ACCEPT
Summary: ArgE provides a hydrolytic route from N-acetyl-L-ornithine to ornithine during L-arginine synthesis.
Reason: The argE gene assignment, EC 3.5.1.16, and ArgE-specific HAMAP/NCBIfam families agree. A KT2440 argE mutant remains arginine prototrophic, consistent with compensation by the alternative ArgJ route rather than evidence against this pathway role.
GO:0008777 acetylornithine deacetylase activity
IEA
GO_REF:0000120 		ACCEPT
Summary: This is the substrate-specific molecular function predicted for ArgE.
Reason: EC 3.5.1.16, HAMAP MF_01108, NCBIfam TIGR01892, and the ArgE M20A subfamily agree.
Supporting Evidence:
file:PSEPK/argE/argE-uniprot.txt
Full=Acetylornithine deacetylase
file:PSEPK/argE/argE-deep-research-openscientist.md
N-acetyl-L-ornithine + H₂O → L-ornithine + acetate
GO:0016787 hydrolase activity
IEA
GO_REF:0000120 		MARK AS OVER ANNOTATED
Summary: This root-level hydrolase term is redundant with acetylornithine deacetylase activity.
Reason: GO:0008777 captures the substrate-specific reaction.
GO:0019213 deacetylase activity
IEA
GO_REF:0000002 		MARK AS OVER ANNOTATED
Summary: This generic deacetylase term is redundant with acetylornithine deacetylase activity.
Reason: GO:0008777 captures the biological substrate and reaction.

Core Functions

Hydrolyzes N-acetyl-L-ornithine to release L-ornithine in the linear route to L-arginine.

Molecular Function:
acetylornithine deacetylase activity
Directly Involved In:
L-arginine biosynthetic process
Supporting Evidence:
  • file:PSEPK/argE/argE-uniprot.txt
    Full=Acetylornithine deacetylase
  • file:PSEPK/argE/argE-deep-research-openscientist.md
    N-acetyl-L-ornithine + H₂O → L-ornithine + acetate

References

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Suggested Questions for Experts

Q: Under which growth conditions does ArgE-mediated hydrolysis contribute measurably relative to ArgJ-mediated acetyl recycling in KT2440?

Suggested experts: microbial arginine metabolism

Deep Research

OpenScientist

(argE-deep-research-openscientist.md)

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