| Evidence type | Organism/system | Key finding | Quantitative data (if any) | Publication (authors, year) | URL/DOI |
|---|---|---|---|---|---|
| identity | *Pseudomonas putida* KT2440 | PP_5078 is explicitly annotated as **aroB**, encoding **3-dehydroquinate synthase**; reported as upregulated during recovery from desiccation/rehydration, supporting the locus-to-function mapping for the target gene/protein (pqac-00000015) | Microarray study with triplicate analyses; numeric fold-change not given in excerpt (pqac-00000015) | López-Lara et al., 2020 | https://doi.org/10.1186/s13213-020-01596-3 |
| identity/pathway role | *Pseudomonas putida* KT2440 engineered for muconate production | The native **aroB** in *P. putida* KT2440 is identified as encoding **3-dehydroquinate synthase**; overexpression improved flux to shikimate-pathway-derived muconic acid (pqac-00000000) | Muconate production reached **33.7 g/L**, **0.18 g/L/h**, **46% molar yield** (**92% of theoretical maximum**) in engineered strains that included aroB overexpression (pqac-00000000) | Ling et al., 2022 | https://doi.org/10.1038/s41467-022-32296-y |
| mechanism | Bacterial DHQS/AroB (reviewed across taxa) | DHQS/AroB (EC 4.2.3.4) catalyzes conversion of **DAHP to 3-dehydroquinate (DHQ)**, the second step of the shikimate pathway and first carbocyclic intermediate-forming reaction; belongs to the **sugar phosphate cyclase superfamily** (pqac-00000004, pqac-00000007) | Reaction-level annotation; no organism-specific kinetic value in these excerpts (pqac-00000004, pqac-00000007) | Derrer et al., 2013; Shende et al., 2024 | https://doi.org/10.2741/4155; https://doi.org/10.1039/d3np00037k |
| mechanism/cofactors | Bacterial DHQS/AroB (reviewed across taxa) | Catalysis requires **NAD+** and a **divalent metal ion**; **Co2+** and **Zn2+** are the principal reported cofactors. Mechanism proceeds through oxidation, phosphate elimination, reduction, ring opening, and intramolecular aldol cyclization (pqac-00000004, pqac-00000005, pqac-00000009, pqac-00000010) | Mechanistic sequence of ~5 chemical steps in one active site; catalytic NAD+ use noted (pqac-00000005) | Dev et al., 2012; Derrer et al., 2013; Maeda & Dudareva, 2012 | https://doi.org/10.2174/157489312803900983; https://doi.org/10.2741/4155; https://doi.org/10.1146/annurev-arplant-042811-105439 |
| mechanism/kinetics | *Mycobacterium tuberculosis* DHQS (comparative authoritative source for conserved AroB chemistry) | AroB/DHQS performs multiple transformations in one active site; kinetics and metal dependence support the conserved bacterial mechanism for DHQS enzymes (pqac-00000006) | **KM(DAHP) = 6.3 µM**, **KM(NAD+) = 70 µM**, **kcat ≈ 0.63 s⁻¹**; activity abolished by EDTA and best restored by **Co2+** (pqac-00000006) | Nunes et al., 2020 | https://doi.org/10.3390/molecules25061259 |
| application | *Pseudomonas putida* pABA pathway engineering | Combinatorial engineering identified **aroB** expression as a **significant limiting factor / bottleneck** for para-aminobenzoic acid production in *P. putida*, making DHQS a practical intervention point in strain design (pqac-00000011, pqac-00000012) | Initial strain set produced **2–186.2 mg/L** pABA; second round reached **232.1 mg/L** (reported in related preprint/peer-reviewed continuation) (pqac-00000011, pqac-00000013) | Campos-Magaña et al., 2024; Campos-Magaña et al., 2025 | https://doi.org/10.1101/2024.06.17.599342; https://doi.org/10.1186/s13036-025-00553-5 |
| application | *Corynebacterium crenatum* metabolic engineering | Overexpression of **aroB** with **aroD/aroE** was part of a successful aromatic amino acid engineering strategy, showing broader real-world use of DHQS tuning in microbial biomanufacturing (pqac-00000014) | **6.42 g/L** L-tyrosine in shake flask; **34.6 g/L** in fed-batch; **16.9%** higher at 3:1 glucose:mannitol vs glucose alone (pqac-00000014) | Yang et al., 2024 | https://doi.org/10.1186/s12934-024-02564-1 |
| omics | *Pseudomonas putida* KT2440 under desiccation/rehydration | **aroB/PP_5078** was included among genes in an overrepresented amino-acid biosynthesis response, consistent with its role in aromatic amino acid precursor supply through the shikimate pathway (pqac-00000015) | Upregulation reported after **18 days desiccation** followed by **20 min** or **24 h** rehydration sampling; no fold-change in excerpt (pqac-00000015) | López-Lara et al., 2020 | https://doi.org/10.1186/s13213-020-01596-3 |


*Table: This table compiles key evidence verifying that Pseudomonas putida KT2440 PP_5078 is aroB encoding 3-dehydroquinate synthase, and summarizes its conserved reaction chemistry, cofactors, pathway role, omics support, and quantitative metabolic-engineering applications.*